Báo cáo khoa học: a-Fetoprotein positively regulates cytochrome c-mediated caspase activation and apoptosome complex formation docx

... AFP stimulates formation of the Apaf-1 apoptosome complex, enhances recruitment and activation of procaspase 3 in the complex, and stimulates release of active caspase 3 and 9 from the apoptosome. ... the complexes. Fig. 7. AFP positively regulates formation of Apaf-1 apoptosome in cell-free extracts and promotes caspase activation and release of caspase 3...

Ngày tải lên: 08/03/2014, 08:20

... in decreased proMMP-2 activation and reduced MT1-MMP expression A decrease in the total amount of MMP-2 (72, 64 and 62 kDa bands), as well as in the active MMP-2 forms (64 and 62 kDa bands), was observed ... expression pattern and activation mechanisms. The best-described activation mechanism for MMP-2 is the two-step cell-surface activation in which proMMP-2 is activated in a...

Ngày tải lên: 18/02/2014, 11:20

... hypoxia (0.5% oxygen) and deferoxamine (100 lmolÆL )1 ) clearly induced P4ha1 (four- and sevenfold) and P4ha2 mRNA (seven- and fourfold) and to a lesser extent also PDI mRNA (two- and 1.5-fold), whilst ... chelation and cobalt with those reported by Tanaka and coworkers for cultured astrocytes [15]. Our findings thus on the one hand fully support these previous findings and on...

Ngày tải lên: 20/02/2014, 02:21

... l M Ca 2+ (A) and pH 5.0 (B and C). Autoactivation experiments were carried out asin Fig. 9A (A) and 10(B and C). Samples (2 l M total zymogen in 100 lL) were trichloroacetic acid-precipitated and analyzed ... trypsinogen Properties of autocatalytic activation and degradation and implications in pancreatic diseases Zolta ´ n Kukor, Miklo ´ sTo ´ th* and Miklo ´ s Sahin-To ´...

Ngày tải lên: 17/03/2014, 03:20

... the literature. R. Vieira and M. Poesio use hand-crafted heuris- tics, encoding syntactic information. For exam- ple, the noun phrase “the inequities of the current land-ownership system” is classified ... discourse and the hearer givenness.The resulting taxonomy is summarised below: brand new NPs introduce entities which are both discourse and hearer new (“a bus”), sub- class of them, b...

Ngày tải lên: 31/03/2014, 03:20

... (0.2 lm and 20 lm; Roche Diagnostics), pan caspase inhibitor Z-VAD-FMK (50 lm; Peptide Institute), caspase- 1 and -4 inhibitor Z-YVAD-FMK (50 lm; Bachem, Du ¨ ben- dorf, Switzerland), caspase- 2 ... tract was cleaved by caspase- 3 in vitro and in the human brain tissues [27], and that cleavage at the caspase- 6 site in huntingtin was essential for the HD-related behavioural and...

Ngày tải lên: 15/02/2014, 01:20

... of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, USA Actin dynamics, the polymerization and depolymeriza- tion of actin filaments and formation of ordered ... of breakage and destruction of filaments upon exposure to SDS and potassium iodide (KI) [30], and the decreased tryptic susceptibility of the 60–69 loop in subdomain 2 [31]. Phos...

Ngày tải lên: 19/02/2014, 07:20

... genes CytOX5a and CytOX5b, coding for the two isofoms Va and Vb, parallels that of genes CYC1 and CYC7, which encode iso-1 and iso-2 of yeast cytochrome c, respectively. CytOX 5a and CYC1 are ... resolved as two major complexes, which comigrated with Apo-ferritin and b-amylase. Based on the rates of migration, the slow migrating complex may be a dimmer and the faster migrati...

Ngày tải lên: 20/02/2014, 23:20

... Allochromatium vinosum cytochrome c¢; Ccm, cytochrome c maturation; Dsb, disulfide bond formation; EC b 562 , Escherichia coli cytochrome b 562 ; PHCP, Hydrogenophilus thermoluteolus cytochrome c¢;PHc 552 , ... cyto- chrome c¢ (PHCP) and H. thermoluteolus cytochrome c 552 (PH c 552 ) genes are indicated as c¢ and c 552 , respectively. The arrow and arrowhead indicate the posit...

Ngày tải lên: 06/03/2014, 00:20

... inactivation of Btk and Tec genes in mice cause a severe phenotype resembling XLA. Tec is involved in the regula- tion of both B and T lymphocytes, fine-tuning of TCR ⁄ BCR signaling, and also activation ... T-cell line Jurkat and in two liver cell lines, HepG2 and Huh7 (Fig. 5C, left part, and data not shown). The proteasome and NF-jB inhibitors repress transcription from the...

Ngày tải lên: 07/03/2014, 00:20