Báo cáo khoa học: Local changes in the catalytic site of mammalian histidine decarboxylase can affect its global conformation and stability pptx

... coding for the subunits of CytOX, changes in the microenvironment of the cell may induce alterations in the catalytic efficiency of mammalian enzyme. Selective loss of CytOX subunits I/II and IV might ... [4,5]. The remaining 10 subunits of the mammalian enzyme, namely, IV, Vb, VIa, VIb, VIc, VIIa, VIIb and VIII are encoded by the nuclear genome, synthes...

Ngày tải lên: 20/02/2014, 23:20

... productive binding to the substrate via two elements in ClpA, one in the N-domain and the other in the pore of the ClpA hexamer. In the case of short unstructured peptides or unfolded proteins such ... debate regarding the role of the ClpB N-domain (which shares a common fold with the N-domain of ClpA) in substrate selection [15–17]. One difficulty in un...

Ngày tải lên: 07/03/2014, 05:20

... continue to discuss the solution of these first two types, since it will be contained in that of the main problem, the distinction of nominative from accusative in the feminine and neuter singular ... identical to the dative plural. 3. The nominative form is identical to the ac- cusative in the feminine singular of der and welcher, in the neuter si...

Ngày tải lên: 07/03/2014, 18:20

... construct in which the N-terminus of the OTR is replaced with the corresponding sequence of the V 1a R [10]. The role of the N-terminus has been addressed recently by alanine-scanning mutagenesis of the N-terminus ... arginyl at this locus is a constraining residue which contributes to maintaining the conformational switch of the V 1a R in the off-state. The...

Ngày tải lên: 07/03/2014, 21:20

... blocked. The presence of 20E, however, favors the formation of the AaEcR⁄ AaUSP heterodimer [13], indicating that the regulation of the activity of these proteins occurs through protein–pro- tein interactions, ... reorganization of the follicular epithelium in the resulting adults [40]. DmE75A and DmE75B have been implicated in defining the transition stages 8...

Ngày tải lên: 18/02/2014, 13:20

... this overall value into its component kinetic steps. The findings of this study are therefore of interest to the protein design field in general, but also inform upon how to fast track the design of peptides ... interpretation in distinguish- ing interacting from noninteracting proteins. One Fig. 1. Schematics of library designs. The helical wheel diagram looks down the...

Ngày tải lên: 18/02/2014, 13:20

... pyridine ring and of c-N-methyl- aminobutyrate (CH 3 -4-aminobutyrate) from the pyrrolidine ring of the molecule. Surprisingly, two alternative pathways for the final steps in the catabolism of ... expression of these genes depends on the presence of nicotine in the growth medium and we have determined the enzyme activities of the proteins. Our results demon...

Ngày tải lên: 19/02/2014, 07:20

... shown in Fig. 2, the domains obtained can delineate the variations of the cumulative GC profile and the density distribution of CpG islands more and more accurately with decreasing t 0 . On the other ... the first point occurring during the course of segmentation, and l and r denote that the point occurs in the left and right subsequence, respect- ively....

Ngày tải lên: 19/02/2014, 07:20

... pilocarpine [4,5]. This model can be used to investigate the develop- ment of neuropathology in SE [6]. Despite numerous studies clearly indicating the importance of enzyme activity in the epileptic ... 1307 inefficiency of the electron-carrying components of the mitochondrial transport chain, monoamine degradation, xanthine oxidase reaction, and metabolism of arach...

Ngày tải lên: 19/02/2014, 16:20

... productive binding of steroid substrates: in the first, a (small) sub population of enzyme binds directly the steroid in the reactive conformation, with the remainder of the enzyme binding the unreactive conformation. ... unreactive conformation. Rescue of the unreactive conformation involves release of steroid and re-binding in the reactive conformation...

Ngày tải lên: 20/02/2014, 02:21