Báo cáo khoa học: Efficient inhibition of b-secretase gene expression in HEK293 cells by tRNAVal-driven and CTE-helicase associated hammerhead ribozymes doc

... Efficient inhibition of b-secretase gene expression in HEK293 cells by tRNA Val -driven and CTE-helicase associated hammerhead ribozymes Barbara Nawrot 1 , Slawomir ... this expression system is of interest for endogenous generation of siRNA and activation of the RNAi effect in human cells [45]. Studies on an inhibition of BACE gene expression...

Ngày tải lên: 08/03/2014, 08:20

... been developed to inhibit STAT3, including inhibition of its expression [14,15], inhibition of its dimerization [16,17] and inhibition of its binding to the DNA promoter sequence using decoy oligodeoxynucleotides ... binding is increased in cells treated with IL-6 and, to a lesser extent, in cells treated with IFN-c (Fig. 6C, lanes 1 and 2). On the other hand, bindi...

Ngày tải lên: 18/02/2014, 08:20

... scFvs are engineered proteins that contain the heavy chain and light chain variable domains (V H and V L , respectively) of an antibody connected by a linker [7]. Each domain has an intradomain disulfide ... modification of this method. We used a biotin ligation enzyme from E. coli for biotinylation [9]. A biotin tag sequence was introduced in the middle of the original linker of...

Ngày tải lên: 16/03/2014, 23:20

... small set of labels. Both of these techniques efficiently com- pute the marginals with a significantly reduced runtime, resulting in faster training and decoding of CRFs. This paper presents an efficient ... for Meth- ods 3 and 4. Note that  for Methods 2, 3, and 4 indi- cates an empirical count of features in training set. All experiments were implemented in C++ and exe...

Ngày tải lên: 23/03/2014, 17:20

... ligation by reverse-joined hairpin ribozymes and engineering of twin ribozymes consisting of conventional and reverse-joined hairpin ribozyme units Sergei A. Ivanov, Ste ´ phanie Vaule ´ on and Sabine ... were interested in using reverse-joined hairpin ribozymes as building blocks for the construction of twin ribozymes. In order to evaluate the structural properties...

Ngày tải lên: 20/02/2014, 01:20

... inhibited the expression of VEGF, thereby suppressing the growth of hepatocarcinoma tumors in mice [87]. In addition, no noticeable increase in inflammatory cytokines, including interferon-a and ... worms, insects, plants and humans [1]. A single strand of exogenously introduced double-stranded small interfering RNA (siRNA; 20–30 nucleotides) guides an RNA-inducing silencing...

Ngày tải lên: 06/03/2014, 01:23

... outgoing edges of a state of the result WSA on demand, without cal- culating all edges in the entire machine, we can maintain a stand -in for the result structure, a ma- chine consisting at first of ... nonterminal n in and returns another WRTG, G out that is different from G in in that it has more productions, specifically those beginning with n in that are in M (G)  . Algo...

Ngày tải lên: 17/03/2014, 00:20

... form: w n-1 1 w n 2 w n :u The n-gram lattice of order n is called E n and is found by composing E ◦Φ n , projecting on the out- put, removing ǫ-arcs, determinizing, and minimis- ing. The construction of E n is fast even ... gain in Equation (5). For ex- ample, in counting paths that contain u 1 , Ψ L n re- tains the first occurrence of u 1 and maps every other symbol to ǫ. T...

Ngày tải lên: 23/03/2014, 16:20

... ‘category’ instead of ‘lexi- cal category’ for brevity 1 . Grouping of words into categories is useful in it- self (e.g., for the construction of thesauri), and can serve as the starting point in many ... corpus, Dmoz (Gabrilovich and Markovitch, 2005), is a web cor- pus obtained by crawling and cleaning the URLs in the Open Directory Project (dmoz.org), result- ing in 6...

Ngày tải lên: 23/03/2014, 18:20

... can be combined freely). Finally, we obtain the number of solutions for a subgraph by adding the numbers of solutions of all its splits. This computation takes linear time in the size of the chart. 10 every x some y book y student x read x,y every x some y book y student x read x,y every x some y book y student x read x,y every x some y book y student x read x,y ✓ ... solving Task...

Ngày tải lên: 31/03/2014, 03:20