Báo cáo khoa học: Efﬁcient inhibition of b-secretase gene expression in HEK293 cells by tRNAVal-driven and CTE-helicase associated hammerhead ribozymes doc

... Efﬁcient inhibition of b-secretase gene expression in HEK293 cells by tRNAVal-driven and CTE-helicase associated hammerhead ribozymes Barbara Nawrot1, Slawomir ... this expression system is of interest forendogenous generation of siRNA and activation of theRNAi effect in human cells [45]. Studies on an inhibition of BACE gene expression by endogenously generatedsiRNA ... ribozymes in HEK293 cells At ﬁrst we checked the activity of the ribozymes by determining the level of the target mRNA in tested cells transfected with ribozyme encoding plasmids. Two sitesof...

Tài liệu Báo cáo khoa học: Efﬁcient killing of SW480 colon carcinoma cells by a signal transducer and activator of transcription (STAT) 3 hairpin decoy oligodeoxynucleotide – interference with interferon-c-STAT1-mediated killing pdf

... beendeveloped to inhibit STAT3, including inhibition of its expression [14,15], inhibition of its dimerization [16,17] and inhibition of its binding to the DNA promotersequence using decoy oligodeoxynucleotides ... binding isincreased in cells treated with IL-6 and, to a lesserextent, in cells treated with IFN-c (Fig. 6C, lanes 1 and 2). On the other hand, binding of phospho-STAT1is detected only in cells ... Constitutive activation of JAK3 ⁄ STAT3 in colon carcinoma tumors and cell lines: inhibition of JAK3 ⁄ STAT3 signaling induces apoptosis and cell cycle arrest of colon carcinoma cells. Am JPathol...

Báo cáo khoa học: Efﬁcient synthesis of a disulﬁde-containing protein through a batch cell-free system from wheat germ pdf

... scFvsare engineered proteins that contain the heavy chain and light chain variable domains (VH and VL, respectively) of an antibody connected by a linker [7]. Each domain hasan intradomain disulﬁde ... modiﬁcation of this method. We used a biotin ligationenzyme from E. coli for biotinylation [9]. A biotin tagsequence was introduced in the middle of the originallinker of scFvLH by PCR (Fig. 3A), and ... containing 1.2 mMATP,0.25 mMGTP, 15 mMcreatine phosphate, 0.4 mMspermi-dine, 28 mMHepes/KOH (pH 7.8), 0.23 mMeach of 20 amino acids (including leucine), 1.8 mgÆmL)1creatinekinase,...

Báo cáo khoa học: "Efﬁcient Inference of CRFs for Large-Scale Natural Language Data" docx

... smallset of labels. Both of these techniques efﬁciently com-pute the marginals with a signiﬁcantly reduced runtime,resulting in faster training and decoding of CRFs.This paper presents an efﬁcient ... for Meth-ods 3 and 4. Note that  for Methods 2, 3, and 4 indi-cates an empirical count of features in training set. Allexperiments were implemented in C++ and executed in Windows 2003 with ... Quad-Core pro-cessor and 8.0 Gbyte of main memory.We ﬁrst show that our method is efﬁcient for learningCRFs (Figure 1). In all learning curves, Dense gener-ally has a higher training log-likelihood...

Tài liệu Báo cáo khoa học: Efﬁcient RNA ligation by reverse-joined hairpin ribozymes and engineering of twin ribozymes consisting of conventional and reverse-joined hairpin ribozyme units ppt

... ligation by reverse-joined hairpin ribozymes and engineering of twin ribozymes consisting of conventional and reverse-joined hairpin ribozyme unitsSergei A. Ivanov, Ste´phanie Vaule´on and Sabine ... were interested in using reverse-joined hairpin ribozymes as building blocks for the construction of twin ribozymes. In order to evaluate the structuralproperties of reverse-joined hairpin ribozymes ... of two domains joined by a single-stranded linker of eight adenosine residues and binds the 3¢-terminal part of its substrate via aduplex of 5 bp (Fig. 2A). The second part of the twinribozyme...

Báo cáo khoa học: Efﬁcient and targeted delivery of siRNA in vivo pdf

... inhibited the expression of VEGF, thereby suppressing the growth of hepatocarcinoma tumors in mice [87]. In addition,no noticeable increase in inﬂammatory cytokines,including interferon-a and ... worms, insects, plants and humans [1]. A single strand of exogenously introduceddouble-stranded small interfering RNA (siRNA; 20–30nucleotides) guides an RNA-inducing silencing proteincomplex ... and obvious effective-ness of RNAi in vitro, silencing target gene expression in vivo using smallinterfering RNA (siRNA) has been a very challenging task due tomultiscale barriers, including...

Báo cáo khoa học: "Efﬁcient Inference Through Cascades of Weighted Tree Transducers" ppt

... outgoing edges of a state of the result WSA on demand, without cal-culating all edges in the entire machine, we canmaintain a stand -in for the result structure, a ma-chine consisting at ﬁrst of ... nonterminal n in and returns another WRTG,Goutthat is different from G in in that it has moreproductions, speciﬁcally those beginning with n in that are in M (G). Algorithms using stand-insshould ... case, G is a stand -in forM(G), analogous to the stand-ins for WSAs and WSTs described in Section 2.Algorithm 3, PRODUCE, takes as input aWRTG G in = (N in , ∆, P in , n0,M, G) and a de-sired...

Báo cáo khoa học: "Efﬁcient Path Counting Transducers for Minimum Bayes-Risk Decoding of Statistical Machine Translation Lattices" pptx

... form:wn-11wn2wn:uThe n-gram lattice of order n is called En and isfound by composing E ◦Φn, projecting on the out-put, removing ǫ-arcs, determinizing, and minimis-ing. The construction of Enis fast even ... gain in Equation (5). For ex-ample, in counting paths that contain u1, ΨLnre-tains the ﬁrst occurrence of u1 and maps everyother symbol to ǫ. This ensures that in any pathcontaining ... an efﬁcient imple-mentation of linearised lattice minimumBayes-risk decoding using weighted ﬁnitestate transducers. We introduce transduc-ers to efﬁciently count lattice paths con-taining...

Báo cáo khoa học: "Efﬁcient Unsupervised Discovery of Word Categories Using Symmetric Patterns and High Frequency Words" ppt

... ‘category’ instead of ‘lexi-cal category’ for brevity1.Grouping of words into categories is useful in it-self (e.g., for the construction of thesauri), and canserve as the starting point in many ... corpus, Dmoz(Gabrilovich and Markovitch, 2005), is a web cor-pus obtained by crawling and cleaning the URLs in the Open Directory Project (dmoz.org), result-ing in 68GB containing about 8.2G words ... viewed as an in- stance of our problem where the desired categoriescontain words that are names of entities of a par-ticular kind, as done in (Freitag, 2004) using co-clustering. Many Information...

Báo cáo khoa học: "Efﬁcient solving and exploration of scope ambiguities" pot

... can be combined freely). Finally, weobtain the number of solutions for a subgraph by adding the numbers of solutions of all its splits. Thiscomputation takes linear time in the size of the chart.10everyxsomeybookystudentxreadx,yeveryxsomeybookystudentxreadx,yeveryxsomeybookystudentxreadx,yeveryxsomeybookystudentxreadx,y✓ ... solving Task 1 above (about 100.000 readings persecond on a modern PC). It achieves this by im-plementing an efﬁcient algorithm for solving dom-inance graphs (Bodirsky et al., 2004) and cachingintermediate ... enumerating them thatwe even know that (1) has trillions of readings; evenutool would take about a year to enumerate and count the readings individually.utool is implemented in C++, efﬁcient and portable,...

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