Báo cáo khoa học: Mathematical modelling of the urea cycle A numerical investigation into substrate channelling docx

... light on the quantitative aspects of this phenomenon in the urea cycle and other pathways. Keywords: arginase; mathematical modeling; metabolite channeling; urea cycle. There has been considerable ... are not always intuitive. The construction of detailed, and necessarily complex, mathematical models serves as a ÔtoolÕ to facilitate analysis of channelling in bioch...

Ngày tải lên: 08/03/2014, 08:20

... procedures Cloning and expression of the Nogo -A fragments The Nogo -A cDNA (designated KIAA 0886) was obtained from the Kazusa DNA Research Institute (Kazusa- Kamatari, K isarazu, Chiba, Japan). A DNA fragment encoding ... Schematic representation of the doma in organization o f the hum an Nogo -A protein. (A) T he do main o rganizat ion of human Nogo -A s howing the...

Ngày tải lên: 16/03/2014, 16:20

... P. & Beaudet, A. L. (1999) Hepatocyte gene therapy in a large animal: a neonatal bovine model of citrullinemia. Proc. Natl Acad. Sci. USA 30, 3981–3986. 177. Patejunas, G., Bradley, A. , Beaudet, ... 1899 REVIEW ARTICLE Argininosuccinate synthetase from the urea cycle to the citrulline–NO cycle Annie Husson, Carole Brasse-Lagnel, Alain Fairand, Sylvie Renouf and Alain La...

Ngày tải lên: 31/03/2014, 01:20

... assessment of the glyoxalase pathway in Leishmania infantum as a therapeutic target by modelling and computer simulation Marta Sousa Silva 1 , Anto ´ nio E. N. Ferreira 1 , Ana Maria Toma ´ s 2,3 , Carlos ... glyoxalase pathway as a potential therapeutic target by revealing the importance of critical parameters of this pathway in Leishma- nia infantum. A sensitivity analysis...

Ngày tải lên: 23/03/2014, 13:20

... bound to the metal, in the company of a water oxygen, from the apical positions. The manga- nese was only 0.06 A ˚ out of the equatorial plane (Table 3). The angles around the metal cofactor sug- gested ... hyper-thermophilic crenarchaeon, Aeropyrum pernix K1. DNA Res 6, 83–101. 11 Nakamura T, Yamamoto T, Inoue T, Matsumura H, Kobayashi A, Hagihara Y, Uegaki K, Ataka M,...

Ngày tải lên: 14/02/2014, 22:20

... cannot, however, explain the remarkably broad wings of the signal. The broad lineshape and the enhanced relaxa- tion properties of the signal at 77 K indicate that the Y Æ is coupled to a paramagnetic ... nrdF + gene was sequenced by a primer walking approach. For DNA analysis, dnastar software (DNAS- TAR Inc., Madison, WI, USA) and clone manager 5.0 (Scientific & Educa...

Ngày tải lên: 15/02/2014, 01:20

... (AK; EC 2.7.2.4) is an enzyme that catalyzes the first committed step, the phosphorylation of the c-carboxyl group of aspartate, of the biosynthetic pathway of the aspartic acid group amino acids ... differential scanning calorimetry; EcAKIII, aspartate kinase III from Escherichia coli; MAD, multiwavelength anomalous diffraction; MjAK, aspartate kinase from Methanococcus jann...

Ngày tải lên: 18/02/2014, 08:20

... Journal compilation ª 2007 FEBS pCX4pur vectors. We thank Toshiko Maeta and Taka- shi Nakamura for their technical assistance. Drs Kazu- hito Naka (Kanazawa University) and Yasuo Ariumi (Okayama ... the inhibitory effect of NS3- 4A on the Cardif-mediated signaling pathway and the protease activity of NS3- 4A remains unclear. Furthermore, we have no evidence that all NS3-4As derive...

Ngày tải lên: 18/02/2014, 16:20

... by the addition of excess unlabeled 5¢ exon product as the chase. (B) A plot of the disappearance of substrate in a normal reaction (no chase, closed circles) and reap- pearance of the substrate ... 1) [14,17,21]. Dependence of substrate cleavage on pH It has been reported that the rate of the substrate- cleavage step in Tetrahymena [22–25], Anabaena [14] a...

Ngày tải lên: 18/02/2014, 18:20

... on surface analysis, we propose a rigid domain association for the N-terminal SEA domain with the back site of the proteinase domain. Abbreviations HAI, hepatocyte growth factor activator inhibitor; ... regions of these proteinases medi- ate interaction of the two b-barrels, formation of the catalytic machinery and structures required for binding of the main chain of...

Ngày tải lên: 19/02/2014, 00:20