Báo cáo khoa học: Kinetic mechanisms of glycine oxidase fromBacillus subtilis docx

... binding properties of glycine oxidase profoundly distinguish it from D -amino acid oxidase. In the present study we investigated the kinetic properties of B. subtilis glycine oxidase using three ... understanding of the evolution and the mode of functioning of this class of enzymes. The main goal of this project was to elucidate the structure-function relationships...

Ngày tải lên: 08/03/2014, 08:20

... of ingested pathogen Release of digested material by exocytosis Fig. 1. Detailed structure of a macrophage showing a typical process of phagocytosis. Phagosome Lysosome Inhibition of fusion of ... candidates for the treatment of the disease. A variety of mechanisms have been suggested to con- tribute towards the survival of Mycobacterium within macrophages. These mechanis...

Ngày tải lên: 16/02/2014, 15:20

... (i.e. deprotonation of C2a-OH of HEThDP and proton- ation of FAD at N5 and N1). The tight and rigid binding of both cofactors excludes a direct carbanion mechanism with covalent linkage between C2a of the HEThDP ... will be kinetically stabilized when k red 2 » k red 1 . Initial Fig. 5. Stereo drawing of the active site of pyruvate oxidase from L. plantarum (Protein Data Bank...

Ngày tải lên: 18/02/2014, 08:20

... hydrolysis of o-NTFNAC was recorded at 430 nm (e = 3954 m )1 Æcm )1 ) for 5 min. Acetyl esters Kinetic analysis of the BuChE-catalysed hydrolysis of o-NPA was followed by monitoring the rate of appearance of ... active site concentration of wild-type BuChE was 0.16 ± 0.01 lm, and that of the D70G mutant was 0.196 ± 0.02 lm. Effects of ligands on steady-state kinetics The eff...

Ngày tải lên: 18/02/2014, 17:20

... constant of the 5¢ exon mimic, h is the fraction of 5¢ exon mimic bound to the ribozyme, and [ribozyme] u is the concentration of unbound ribozyme in the reaction. Measurement of rate constant of substrate-cleavage ... property of nuclear group I introns. RNA 9, 1464–1475. 6 Herschlag D & Cech TR (1990) Catalysis of RNA cleavage by the Tetrahymena thermophila ribozyme. 1. K...

Ngày tải lên: 18/02/2014, 18:20

... spectrum of the triply phosphorylated SRSRpT 212 PpS 214 LPpT 217 PPTR peptide of Tau. The cis conformation for the Pro219 is below the limit of detection. Upon addition of a catalytic amount of Pin1, ... molecular mechanisms of the enzyme action. Most importantly, we want to critically review the evidence that Pin1 would or would not act as a prolyl cis ⁄ trans isomerase. Molec...

Ngày tải lên: 07/03/2014, 05:20

... development of new chemotherapeutics against amoebiasis. For the further development of antiamoebic agents based on TFM, elucidation of the underlying reaction mechanisms of MGLs and the interaction of ... Samarawickrema NA, Brown DM, Upcroft JA, Tham- mapalerd N & Upcroft P (1997) Involvement of super- oxide dismutase and pyruvate:ferredoxin oxidoreductase in mechanisms...

Ngày tải lên: 07/03/2014, 05:20

... activation kinetics, i.e. the time dependence of the development of phenoloxidase activity after the addition of SDS, were studied (Fig. 1). After the addition of SDS, the catecholoxi- dase activity of ... amongst arthropod phenoloxidases, but has not been reported previously simply because of a lack of studies. The inhibition of activated hemocyanin by typical inhibi- tors o...

Ngày tải lên: 07/03/2014, 05:20

... stability of IN during the time-course of the reaction and the influence of protein aggregation are discussed. Results General features of the 3¢-end processing kinetics The kinetics of 3¢-end ... using image quant 4.1 software. Analysis of kinetic data and determination of apparent K m and k cat values (K m ¢ and k cat ¢) For 3¢-processing kinetic study, the amount of 19-m...

Ngày tải lên: 16/03/2014, 13:20

... chemicals were local products of analytical grade. In kinetic studies of the autocatalytic conversion of trypsinogen into trypsin, aliquots of the incubation mixture of trypsinogen and trypsin were ... detailed kinetic analysis of autocatalytic reaction modulated by a reversible inhibitor is represented. On the basis of the kinetic equation, a novel procedure is developed...

Ngày tải lên: 23/03/2014, 13:20