Báo cáo khoa học: Purification of a plant nucleotide pyrophosphatase as a protein that interferes with nitrate reductase and glutamine synthetase assays pdf

... Purification of a plant nucleotide pyrophosphatase as a protein that interferes with nitrate reductase and glutamine synthetase assays Greg B. G. Moorhead*, Sarah E. M. Meek, Pauline Douglas*, ... glutamine synthetase (GS) and nitrate reductase (NR) was highly purified from cauli- flower (Brassica oleracea var. botrytis) extracts. The final preparatio...

Ngày tải lên: 08/03/2014, 08:20

... crosspeaks were assigned and inte- grated, with concomitant cycles of structure calcula- tions for evaluation of distance and angle constraint violations as well as assignments of additional peaks based ... China Sea. Sephadex G-25 was purchased from Amersham Biosciences (Uppsala, Sweden), a ZORBAX 300SB-C18 semipreparative column was from Agilent Tech- nologies (Santa Clara...

Ngày tải lên: 18/02/2014, 17:20

... ATCCTCACGAACAAGCAG 5RACR GATCGCGATGCAGGCCTT FLF1 GGACGACTACAGCGTCTTCAGTAGA FLR1 TCCAAACAGTCAGTTTCTTAACCGT Table 1. Purification of cellulase from abalone Haliotis discus hannai. Oneunitofcellulasewasdefinedastheamountofenzymethatliberates reducing ... et al. [29] using bovine serum albumin fraction V as a standard protein. cDNA cloning Construction of the cDNA library and cloning...

Ngày tải lên: 20/02/2014, 23:20

... sialidasetypeX,protease enzymes and molecular mass standards were purchased from Sigma. Preparation of crab sera Freshwater field crabs, Paratelphusa jacquemontii were collected from the local ... were washed in NaCl/Tris five times and used for the hemagglu- tination assay. Preparation of asialo-erythrocytes. The procedure fol- lowed for preparing asialo-erythrocytes was that of Me...

Ngày tải lên: 21/02/2014, 00:20

... subunit of nitrate reductase from various organisms, such as NarI of nitrate reductase of Escherichia coli [26]. AF501 also has low sequence similarity to the b-type cytochrome (HdrE) of Hdr. A topological ... polypeptide with an apparent molecular mass of 53 kDa appears as a double band in unboiled samples (lanes A1 and B1). Table 1. N-Terminal sequences of t...

Ngày tải lên: 21/02/2014, 03:20

... 216 73TTREEQPAAEAAASTAGGSQ ACC ACC AGG GAA GAA CAA CCG GCG GCA GAG GCC GCG GCG TCC ACC GCT GGC GGT AGC CAA 276 93QEGYGSTQLPSDEPLNGLND CAA GAA GGA TAT GGC AGC ACC CAA CTT CCA TCG GAC GAG CCA TTG AAC GGG ... GGA GGC TAT GGC GGA GAG GGA TCA GCA GCC GCC CAG GAG GTC CAG ACC GCC GCC 156 53QEAVEGAEQVASESASLTTP CAG GAG GCG GTA GAG GGC GCC GAG CAG GTA GCG TCC GAG TCA GCC TCC CTC ACA ACA CCA 216 73TTRE...

Ngày tải lên: 07/03/2014, 12:20

... compo- nents of each reaction mixture, after their chromatographic separation and purification, were submitted to 1 HNMR (nuclear magnetic resonance) and FABMS (fast atom bombardment mass spectrum) analyses ... confirmed that it consists actually of a unique enzymatic protein. Molecular mass and pI determination of F 4¢-OMT. The molecular mass of the pure enzyme was calcula...

Ngày tải lên: 08/03/2014, 08:20

... pGEX–EWS; EAD forward d(CGG AAT TCA TGG CGT CCA CGG ATT ACA G) and EAD reverse d(CGC TCG AGT CAT CCG GAA AAT CCT CCA GAC T), for pGEX–EAD; RGG1 forward d(CGG AAT TCC CAG GAG AGA ACC GGA GCA T) and RGG1 ... compilation ª 2011 FEBS Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein Kentaro Takahama 1, *, Katsuhito Kino 2, *, Shigeki Arai 3 ,...

Ngày tải lên: 15/02/2014, 01:20

... AAG CCG ATG ACA CCA ATT (asd sense) This study asd2 GCA GGT TCA TAG TGC ATG (asd antisense) This study Kan RP GGT GCG ACA ATC TAT CGA (kanamycin sense) [19] Kan FP CTC ATC GAG CAT CAA ATG (kanamycin ... mean value was calculated. Bactericidal assay with normal human serum Complement-sufficient normal human serum was prepared and pooled from eight healthy adult donors. A bactericidal assa...

Ngày tải lên: 18/02/2014, 14:20

... region was decreased and a new band was detected at 64 kDa, but only after treatment with heparitinase II (Fig. 5A) . Although the same band was obtained after digestion Fig. 3. 45 Ca overlay analysis ... found to have a molecular mass of 64 kDa, and to contain two tandem repeats and a Glu-rich region. The structure of the protein and that of its DNA are similar to t...

Ngày tải lên: 18/02/2014, 17:20