Báo cáo khoa học: Connection of transport and sensing by UhpC, the sensor for external glucose-6-phosphate in Escherichia coli ppt

... arginine residues are important for sensing and not for transport, and that residues located in the central hydrophilic loop are critical for transport and for sensing. Keywords: Escherichia coli; ... mutations of the UhpC protein on both the transport and sensing functions of this molecule. We mutated arginine residues as these are known to be involv...

Ngày tải lên: 08/03/2014, 08:20

... [28–31]. However, the nature of the photoprotective mechanism has remained elusive. Further studies of the functions of GFP-like proteins are necessary for assessing the adaptability of coral ecosystems in ... respectively. The amounts of protein in the calib- ration solutions and the cleared tissue extracts were deter- mined following the manufacturer’s inst...

Ngày tải lên: 16/03/2014, 11:20

... S6 kinase and glycogen synthase kinase-3 activity in L6 muscle cells: evidence for the involvement of the mammalian target of rapamycin (mTOR) pathway in the L -leucine-induced up-regulation of ... of insulin is to promote the uptake, metabolism and storage of glucose in adipose tissue and skeletal muscle [1]. The hormonal regulation of these cellular proce...

Ngày tải lên: 08/03/2014, 08:20

... 1B shows the pattern seen after the development of the blot. With increase in time of incubation, the intensity of the band for the monomeric protein decreased, whereas the bands for the higher ... solid line), in the presence of 100 l M neurotoxin ( • , dotted line), in the presence of 200 l M neurotoxin ( , dotted line) and in the presence of...

Ngày tải lên: 14/02/2014, 19:20

... latter depends on (a) the structural integrity of the vascular bed of the penis; (b) the endothelium lining the cavernosal arteries and the helicine arterioles, as well as the endothelium lining the lacunar ... serve as an endocrine disrupter and in uence the endocrine milieu by reducing the levels of luetinizing hormone (LH) and testosterone resulting in...

Ngày tải lên: 18/02/2014, 06:20

... sequences at the N-terminus and C-terminus of the protein, respectively. These sequences match to the 50S ribo- somal protein, L34. Lines above and below the protein sequence indicate the amino acid ... complementary pair of fragment ions of type c¢ and z¢ Æ . Subtraction of the m ⁄ z values for the frag- ments within a given ion series that differ by a single...

Ngày tải lên: 18/02/2014, 16:20

... uptake. Eadie– Hofstee plots of initial rates of uptake of L-[1- 14 C]arabinose in 0.5% L-arabinose-grown cells of C. arabinofermentans PYCC 5603 T ,in the absence (j) and in the presence of 50 m MD-xylose ... estimated. In addition, l-arabinose ⁄ proton symport activity was evaluated by determining the rate of proton in ux associated with the transport of...

Ngày tải lên: 19/02/2014, 00:20

... using antibodies that bind to the N-terminal region, whereas the other exhibits high intensity for protein bands at the size of the nonglycosylated isoform using antibodies recognizing the C-terminal region. ... binding antibodies. The signal intensity of each of the three protein bands was quantified by densitometry. The mean values of the calculated signal inten...

Ngày tải lên: 19/02/2014, 02:20

... component of the cell–cell adhesion complex and required for the for- mation of epithelia in the embryo and the maintenance of the polarized epithelial structure in the adult [2]. As a single-span ... a nonredundant and isoform-specific role for Shc in the Erk signaling induced by the E-cadherin blockage. Decma-induced Shc tyrosine phosphorylation and...

Ngày tải lên: 19/02/2014, 05:20

... cathepsin G [3]). The two former proteins are mainly synthesized in the liver and reach the lung via the blood circulation. They are irreversible inhibitors that belong to the serpin family. Their ... of Pr3 by the oxidized inhibitors. While the above data are in overall agreement with the results obtained using synthetic substrates, they also indicate that elastin h...

Ngày tải lên: 19/02/2014, 07:20