Báo cáo khoa học: In situ proton NMR analysis of a-alkynoate biotransformations ppt
Báo cáo khoa học: In situ proton NMR analysis of a-alkynoate biotransformations ppt
... In situ
proton NMR analysis of a-alkynoate biotransformations
From ‘invisible’ substrates to detectable metabolites
Lothar Brecker
1,
*, Julia Petschnigg
1
, Nicole Depine
´
1
, Hansjo¨ ... and analysis of the fermentation time
courses [11–15]. While, except in propynoate, the triple
bond in a-alkynoates do not carry a proton, the substrates
are ÔinvisibleÕ in
1
H -...
Báo cáo khoa học: Computer-assisted mass spectrometric analysis of naturally occurring and artificially introduced cross-links in proteins and protein complexes potx
... rigid protein breaks up into MALDI-MS detectable frag-
ments, leaving the disulfide bonds intact. We also show the mapping of a
number of chemically introduced cross-links in the NK1 domain of hepato-
cyte ... major tool in the
structural analysis of proteins and protein complexes
and in large scale analysis of the function of genes
(proteomics) [1].
For mass spectrometri...
Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf
... of a JC virus pentanucleo-
tide repeat element binding protein: cellular nucleic acid
binding protein. Virus Res 58, 73–82.
6 Flink IL & Morkin E (1995) Alternatively processed
isoforms of ... November
2006)
doi:10.1111/j.1742-4658.2006.05596.x
The zinc-finger cellular nucleic acid binding protein (CNBP) is a strikingly
conserved single-stranded nucleic acid binding protein essential f...
Báo cáo khoa học: Identification and expression analysis of an IL-18 homologue and its alternatively spliced form in rainbow trout (Oncorhynchus mykiss) doc
... c.secombes@abdn.ac.uk
Abbreviations: IL, interleukin; IL-1Ra, interleukin 1 receptor
antagonist; ICE: interleukin 1b converting enzyme; IL)18 BP,
interleukin 18 binding protein; IFN, interferon; c-IP10, interferon
gamma induced ... Cloning and expression of interleukin-18
binding protein. FEBS Lett. 445, 338–342.
32. Novick, D., Kim, S.H., Fantuzzi, G., Reznikov, L.L., Dinarello,
C.A. &...
Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx
... Ab
oligomerization [22–25]. Since a functioning zinc-bind-
ing domain may be present in the Ab sequence of C99,
we hypothesized that zinc may affect the oligomeriza-
tion state of C101-3FLAG. Therefore, ... APP
intracellular domain (AICD or e-CTF) by cleaving 9–7
amino acids from c 40 and 42 sites at the e site [1–3].
Several lines of evidence support the pathogenic role
of c-cleava...
Báo cáo khoa học: Genomic structure and expression analysis of the RNase j family ortholog gene in the insect Ceratitis capitata pptx
... manifested by the presence
of multiple ARE-binding proteins, including stabilizing
proteins [29,30] or proteins that promote degradation
of mRNAs that contain AREs [31]. Recent findings
reveal that the ... organization of the ORF
region of the RNase j family genes is shown in
Fig. 7A. In all organisms examined, the region coding
for RNase j is interrupted by two introns, with the
e...
Báo cáo khoa học: Experimental and steady-state analysis of the GAL regulatory system in Kluyveromyces lactis docx
... that of the other species whilst
retaining the parameters of the former. The in silico
re-engineering study indicated that the leaky behavior
in response to glucose inhibition was also obtained in
S. ... requirement of Gal4p for the expression of genes
with two binding sites is a result of the strong cooper-
ativity during the binding of the transcriptional activa-
tor to th...
Báo cáo khoa học: In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase potx
... display),
increasing the likelihood of correct folding in a redu-
cing environment while minimizing the risk of proteo-
lytic degradation. The association of plasmid and
fusion protein can be maintained ... mechanism of the a subunit of tryp-
tophan synthase: an amino-terminal autonomous
folding unit controls several rate-limiting steps in the
folding of a single domain protei...
Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx
... cytokines; interleukin; innate immunity; fish;
expression analysis.
Cytokines play a significant role in initiating and regulating
the in ammatory process, which is an important defense
system in innate ... immunity. Cytokines are subdivided into
families such as interleukins (ILs), lymphokines, growth
factors, interferons (IFNs) and chemokines. IL-10, initially
known as cytokine synthes...
Tài liệu Báo cáo khoa học: A differential scanning calorimetry study of tetracycline repressor pptx
... results in inducer
binding in the protein cavities [33]. These studies show that
substitution of protein residues engaged in hydrogen
bonding with [Tc–Mg]
+
results in reduced binding of the
inducer ... rate of 1 KÆmin
)1
(Figs 1 and 2).
Therefore, under these experimental conditions, binding of
Tc causes an increase in the T
max
of the protein of
% 10.0 °C. The ligand b...