Báo cáo khoa học: In situ proton NMR analysis of a-alkynoate biotransformations ppt

... In situ proton NMR analysis of a-alkynoate biotransformations From ‘invisible’ substrates to detectable metabolites Lothar Brecker 1, *, Julia Petschnigg 1 , Nicole Depine ´ 1 , Hansjo¨ ... and analysis of the fermentation time courses [11–15]. While, except in propynoate, the triple bond in a-alkynoates do not carry a proton, the substrates are ÔinvisibleÕ in 1 H -...

Ngày tải lên: 08/03/2014, 08:20

... rigid protein breaks up into MALDI-MS detectable frag- ments, leaving the disulfide bonds intact. We also show the mapping of a number of chemically introduced cross-links in the NK1 domain of hepato- cyte ... major tool in the structural analysis of proteins and protein complexes and in large scale analysis of the function of genes (proteomics) [1]. For mass spectrometri...

Ngày tải lên: 07/03/2014, 12:20

... of a JC virus pentanucleo- tide repeat element binding protein: cellular nucleic acid binding protein. Virus Res 58, 73–82. 6 Flink IL & Morkin E (1995) Alternatively processed isoforms of ... November 2006) doi:10.1111/j.1742-4658.2006.05596.x The zinc-finger cellular nucleic acid binding protein (CNBP) is a strikingly conserved single-stranded nucleic acid binding protein essential f...

Ngày tải lên: 16/03/2014, 12:20

... c.secombes@abdn.ac.uk Abbreviations: IL, interleukin; IL-1Ra, interleukin 1 receptor antagonist; ICE: interleukin 1b converting enzyme; IL)18 BP, interleukin 18 binding protein; IFN, interferon; c-IP10, interferon gamma induced ... Cloning and expression of interleukin-18 binding protein. FEBS Lett. 445, 338–342. 32. Novick, D., Kim, S.H., Fantuzzi, G., Reznikov, L.L., Dinarello, C.A. &...

Ngày tải lên: 16/03/2014, 16:20

... Ab oligomerization [22–25]. Since a functioning zinc-bind- ing domain may be present in the Ab sequence of C99, we hypothesized that zinc may affect the oligomeriza- tion state of C101-3FLAG. Therefore, ... APP intracellular domain (AICD or e-CTF) by cleaving 9–7 amino acids from c 40 and 42 sites at the e site [1–3]. Several lines of evidence support the pathogenic role of c-cleava...

Ngày tải lên: 16/03/2014, 23:20

... manifested by the presence of multiple ARE-binding proteins, including stabilizing proteins [29,30] or proteins that promote degradation of mRNAs that contain AREs [31]. Recent findings reveal that the ... organization of the ORF region of the RNase j family genes is shown in Fig. 7A. In all organisms examined, the region coding for RNase j is interrupted by two introns, with the e...

Ngày tải lên: 23/03/2014, 06:20

... that of the other species whilst retaining the parameters of the former. The in silico re-engineering study indicated that the leaky behavior in response to glucose inhibition was also obtained in S. ... requirement of Gal4p for the expression of genes with two binding sites is a result of the strong cooper- ativity during the binding of the transcriptional activa- tor to th...

Ngày tải lên: 29/03/2014, 09:20

... display), increasing the likelihood of correct folding in a redu- cing environment while minimizing the risk of proteo- lytic degradation. The association of plasmid and fusion protein can be maintained ... mechanism of the a subunit of tryp- tophan synthase: an amino-terminal autonomous folding unit controls several rate-limiting steps in the folding of a single domain protei...

Ngày tải lên: 30/03/2014, 20:20

... cytokines; interleukin; innate immunity; fish; expression analysis. Cytokines play a significant role in initiating and regulating the in ammatory process, which is an important defense system in innate ... immunity. Cytokines are subdivided into families such as interleukins (ILs), lymphokines, growth factors, interferons (IFNs) and chemokines. IL-10, initially known as cytokine synthes...

Ngày tải lên: 20/02/2014, 02:21

... results in inducer binding in the protein cavities [33]. These studies show that substitution of protein residues engaged in hydrogen bonding with [Tc–Mg] + results in reduced binding of the inducer ... rate of 1 KÆmin )1 (Figs 1 and 2). Therefore, under these experimental conditions, binding of Tc causes an increase in the T max of the protein of % 10.0 °C. The ligand b...

Ngày tải lên: 20/02/2014, 02:21