Báo cáo khoa học: Structural and biological effects of a b2- or b3-amino acid insertion in a peptide Application to molecular recognition of substance P by the neurokinin-1 receptor ppt

... 0for each peptide. The percentage of degradation was calculated by comparing the area of the peaks of the intact peptide at t ¼ 0andt ¼ 60 min. Binding assays Binding assays were carried out at ... 943 expressed as K i for NK-1M (major site) and NK-1m (minor site), and EC 50 values for the cAMP pathway and for the inositol phosphates pathway. SP, [Ala9]SP and [P...

Ngày tải lên: 08/03/2014, 08:20

... catalyzes the transfer of a hypoxanthine or guanine base to a- d-5-phosphoribosyl 1-pyrophosphate (PRPP), pro- ducing IMP or GMP and pyrophosphate. Several of the identified mutations leading to disease ... bonded to main chain and side chain atoms of amino acids 145–149 (Fig. 1C). A second Ca 2+ ion is coordinated by the side chain of Asp201, a phosphate i...

Ngày tải lên: 15/02/2014, 01:20

... comparison, the NOS flavoprotein domain is related to a family of dual-flavin enzymes that contain FAD and FMN, and transfer NADPH-derived elec- trons to separate hemeprotein partners or to attached heme ... and a C-terminal flavopro- tein or reductase domain that binds NADPH, FAD and FMN. The two domains are separated by a CaM-binding motif. During catalysis, NAD...

Ngày tải lên: 18/02/2014, 11:20

... characteristic p- p charge transfer (CT) absorbance and NAD (P) H binding and dissocia- tion can be measured by following the formation of this CT absorbance at, for example, 555 nm while perform- ing ... (wt) and N18 9A mutant of MR with NADH. The wt trace is fit to a single exponential and the N18 9A trace to a 4-exponential function – see the main text for m...

Ngày tải lên: 18/02/2014, 11:20

... protein– flavin complexes and tailor the midpoint potentials in these proteins, as well as many details of the binding and electron transfer to protein and ligand partners, have been revealed. In addition ... subunits of Synechococ- cus elongatus (Sy) PSI, PsaC, PsaD and PsaE, and the extrinsic loop of PsaA, present a positively charged surface potential (Fig. 1A)...

Ngày tải lên: 18/02/2014, 11:20

... a nondomain-swapped dimeric form. Domain swapping is avoided in Pa SurE by a sharp turn in the segment of residues 242–245, bringing the polypeptide chain back to the same subunit. We analyzed the interactions of ... pH on the phosphatase activity of St SurE using pNPP as the substrate. (D) Effect of divalent cations on the phosphatase activity of St SurE u...

Ngày tải lên: 18/02/2014, 14:20

... with ADP and UDP, and ADP showed a rate that was > 20 times that of UDP. In order to determine the true K m for UMP and ATP, a two-substrate assay was performed at four concentrations of UMP and ... from the opportunistic pathogen Ureaplasma parvum was deter- mined and showed similar three-dimensional fold as other bacterial and archaeal UMPKs that all belong t...

Ngày tải lên: 18/02/2014, 16:20

... fiber protein. The penton contains all necessary components for viral attachment and entry into the host cell. After initial attachment via the head domain of the fiber protein, the penton base interacts ... formed by insertions between the strands of the jellyroll domain (Fig. 3). Antiparallel b-sheets made up of strands CHEF and BIDG pack against each other, forming...

Ngày tải lên: 19/02/2014, 06:20

... Gesualdo L, Grandaliano G, Maiorano E & Schena FP (2001) The role of alpha-smooth muscle actin and platelet-derived growth factor-beta receptor in the progression of renal damage in human IgA nephropathy. ... kinase receptors PDGF receptor -a and -b. These two recep- tor isoforms dimerize upon binding the PDGF dimer, leading to three possible receptor combina- t...

Ngày tải lên: 19/02/2014, 07:20

... and urea became more potent inhibitors at low temperature. While typically considered an activator of PFK activity, inorganic phosphate per- formed as an inhibitor at 5 °C. Decreasing temperature ... Assay temperature was manipulated by using the microplate thermal controller for 37 °C assays or by placing the entire microplate reader into a low temperature incuba- tor for 5 °...

Ngày tải lên: 19/02/2014, 16:20