Báo cáo khoa học: Plasmodium falciparum merozoite surface protein 1 Glycosylation and localization to low-density, detergent-resistant membranes in the parasitized erythrocyte pdf

... 375 Plasmodium falciparum merozoite surface protein 1 Glycosylation and localization to low-density, detergent-resistant membranes in the parasitized erythrocyte Daniel C. Hoessli 1 , Monique Poincelet 1 , ... configuration[22]. The remaining 35% of incorporated [3H]GlcN that isresistant to the Jack Bean hexosaminidase could be eitherGPI-linked, N-linked to asparagines or linked to the surface of the protein in ... serines and threonines, using specific antibodies to map the biosynthe-tically labelled modifications to the N and C terminus of the MSP -1 protein [16 ]. The presence of O-GlcNAc on both the C- and...

... were: L2,K 11, Q13, C14, P15 and S18, as shown in Fig. 1. According to these results, the interaction was located mainly at the C-terminus of peptide 55 01. Fine erythrocyte- bindingspecificity ... MSP -1 protein; peptide analogues; nuclearmagnetic resonance; vaccine candidate.Multiple receptor–ligand type interactions are involved in the host-cell invasion process of the Plasmodium falciparum malaria ... doi :10 .10 46/j .14 32 -10 33.2003.03780.x(Bachem, Torrance CA, USA). The resin was deprotonatedby adding 5% diisopropylethylamine (Merck) in methylenechloride before introducing the first amino...

... Ren 1, *, Ailan He 1 , Xiang Hu 1 , Wenfeng Zhang 1 ,Liping Yang 1 , Mingjun Liu 1 , Hong Li 1 , Jianlin Zhou 1 , Shuanglin Xiang 1 and Jian Zhang 1, 2 1 Key Laboratory of Protein Biochemistry and ... the interaction between Sp1 and AP-2 affects formation of the Sp1–DNA complex [23];(c) competing model – the AP-2 and Sp1 binding sitesoverlap, and the AP-2 binding competes with the SP1binding ... regulate KCTD10 R. Liu et al. 11 16 FEBS Journal 276 (2009) 11 14 11 24 ª 2009 Hunan Normal University. Journal compilation ª 2009 FEBSBinding of Sp1 and AP-2a to the correspondingsites in the promoter...

... HPPreveals the involvement of the flexible loop in substratebinding. Protein Sci 8, 10 23 10 31. 42 Focia PJ, Craig SP III & Eakin AE (19 98) Approaching the transition state in the crystal ... 97, 7727–77 31. 36 Wang Z, Mottonen J & Goldsmith EJ (19 96) Kineti-cally controlled folding of the serpin plasminogen acti-vator inhibitor 1. Biochemistry 35, 16 443 16 448.37 Cunningham EL, ... 10 mm and a heating rate of 1 °CÆmin )1 . The guanidinium chloride denaturation meas-urements were carried out at a protein concentration of 10 lm with a path length of 1 mm. Protein unfolding...

... BchI, in a previousstudy [26]. We analysed the ability of these BchI proteins,with the exchanges D207N, R289K and L 111 F, to bindtothe70kDaBchDproteinwithanN-terminalHis-tag.His-tagged proteins ... (20 01) Interplay between anAAA module and an integrin I domain may regulate the functionof magnesium chelatase. J. Mol. Biol. 311 , 11 1 12 2. 11 . Vale, R.D. (2000) AAA proteins: Lords of the ring. ... cytoskeletalregulation, protein folding and proteolysis [11 13 ], and nowalso in porphyrin metallation [10 ]. The N-terminal halfof the BchD subunit is homologous to BchI, while the C-terminal...

... oAthalianaBPativumBSoleraceaBNtabacumBA.thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcusAthalianaBPativumBSoleraceaBNtabacumBA.thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcusAthalianaBPativumBSoleraceaBNtabacumBA.thalianaAPsativumASoleraceaAChlamySynechocystisSynechococcus 11 9 11 9 11 9 11 9 11 9 12 0 11 9 12 1 11 8 11 9 15 9 15 9 15 9 15 9 15 7 15 8 15 7 16 0 15 8 15 9 19 9 19 9 19 9 19 9 19 7 19 8 19 7200 19 8 19 9VI I T A PAK GAD I P T Y VMG V NEQDYGHDVAN ... corresponding to the cofactor-binding domain, orRossman fold (residues 1 14 7 and 313 –334 in spinachGAPDH (accession code in PDB:1JN0), the other being the catalytic domain (residues 14 8– 313 ). The ... method to identify residuespotentially involved in protein p rotein interactions [13 ].This method indicates that among other candidates,residues Lys128 and Arg197 may be involved in protein protein...

... rder to construct an interface be-tween the learnt policies for COMMUNICATO R and the in- car baseline system.5.2 Mapping between COMMUNICATOR and the In- car DomainsThere are 2 main problems to ... locations in the town, and the dialogue system willbe used to specify and refine the user queries, as wellas to present information to the user. See the exampledialogue in table 1. We now describe the ... examples of their use. We then discuss the important features of the system in respect to the dialogue phenomena that they support. 1. 1 A System Exhibiting Reinforcement Learning The central...

... (lgÆlL )1 ) n 5th 6th 7th 8thEGFP 1. 5–2.0 22 – – 18 45.0 13 – – 12 1 TcMT1 1. 5 6 – – 3 3TcMT2 1. 7 10 – – 4 6TcMT3 1. 5–2.0 15 – 12 2 – Pupal arrest (5th), 1 5.0 19 11 42 – Prepupal arrest (5th), 1; ... expressedspecifically in CA until the beginning of the final larvalinstar [9]. In contrast, the BmJHAMT transcript isundetectable in CA at the beginning of the spinningstage, but is detected at low levels in the ... background. The putative SAM-binding motif(motif I) is boxed. The positions of the introns are indicated by red lines, and the numbers above indicate the positions of the introns asdescribed in the...

... &Broaddus, V.C. (20 01) Tumor necrosis factor-related apoptosis-inducing ligand and chemotherapy cooperate to induce apoptosis in mesothelioma cell lines. Am.J.Respir.CellMol.Biol.25, 11 1 11 8.2730 L. ... promotes the activation of caspases, such as procaspase-9 and caspase-3,by binding to the inhibitor of apoptosis proteins and thusdisrupts linkage of the caspase–inhibitor of apoptosisproteins ... weremaintained in RPMI -16 40 containing 10 % heat-inactivatedbovine serum, 1 mML-glutamine, 10 0 UÆml )1 penicillin and 10 0 lgÆml )1 streptomycin (Life Technologies, Inc. GrandIsland, NY, USA) at 37 °C...

... 2959SbPGP220 1 23456SbPIN105 10 15 20SbPIN205 10 15 20SbPIN400. 01 0.020.030.040.050.060.07SbPIN500 .1 0.20.30.4SbPIN60 10 0020003000400050006000SbPIN70 1 234567SbPIN800.5 1 1.522.5SbPIN905 10 15 20SbPIN10050 10 0 15 0200SbLAX105 10 15 20253035SbLAX202468 10 12 SbLAX300.050 .1 0 .15 0.2SbLAX400.5 1 1.522.53SbLAX5020406080 10 0SbPGP100.5 1 1.522.5SbPGP200. 01 0.020.030.040.05SbPGP300 .1 0.20.30.40.50.6SbPGP400.20.40.60.8 1 SbPGP500. 01 0.020.030.040.05SbPGP805 10 15 202530SbPGP900.020.040.060.080 .1 SbPGP1000.050 .1 0 .15 0.20.250.3SbPGP 11 020406080 10 0 12 0SbPGP120 1 2345SbPGP1300.020.040.060.080 .1 0 .12 0 .14 SbPGP140 1 234SbPGP1500.050 .1 0 .15 0.2SbPGP1602468 10 12 SbPGP1700.5 1 1.522.53SbPGP1800.20.40.60.8 1 1.2SbPGP1902468 10 12 14 SbPGP2000 .1 0.20.30.4SbPGP 21 00.5 1 1.522.533.5SbPGP2300.0050. 01 0. 015 0.020.0250.03SbPGP2400.050 .1 0 .15 0.20.250.3SbPIN 11 0 10 20304050RNA ... levelLeafRoot05 10 15 0200250SbPIN 1 SbPIN2SbPIN3SbPIN4SbPIN5SbPIN6SbPIN7SbPIN8SbPIN9SbPIN10SbPIN 11 05 10 15 20SbLAX1SbLAX2SbLAX3SbLAX4SbLAX505 10 15 2025SbPGP1SbPGP2SbPGP3SbPGP4SbPGP5SbPGP6SbPGP7SbPGP8SbPGP9SbPGP10SbPGP1 1 SbPGP 12 SbPGP13SbPGP14SbPGP 15 SbPGP16SbPGP17SbPGP18SbPGP19SbPGP20SbPGP 21 SbPGP22SbPGP23SbPGP24IAA ... the downloaded proteome annota-tions. Any protein containing a Mem_trans domain(PF03547 .11 ) was chosen, and after removing the PIN-likeproteins, the remaining proteins were considered SbPINproteins....