Báo cáo khoa học: Signaling events mediating activation of brain ethanolamine plasmalogen hydrolysis by ceramide pot

... E. Latorre et al.(Eur. J. Biochem. 270) Ó FEBS 2003 Signaling events mediating activation of brain ethanolamine plasmalogen hydrolysis by ceramide Eduardo Latorre 1 , M. Pilar Collado 1 , Inmaculada ... that activation of hydrolysis of Etn phospholipids by PLA 2 results from the covalent modification of the enzyme [38]. In an attempt to establish the potential...

Ngày tải lên: 08/03/2014, 08:20

... binding results in activation of brain SR. J Biol Chem 277, 27782–27792. 10 Neidle A & Dunlop DS (2002) Allosteric regulation of mouse brain SR. Neurochem Res 27, 1719–1724. Activation of brain SR by GRIP, ... of a two-fold molar excess of the PDZ6 domain of GRIP under different assay condi- tions. Data are representative of three independent experiments. F. Baum...

Ngày tải lên: 30/03/2014, 03:20

... proform localizes to the surfaces of the gonad, intestine and hypodermis corresponding to the basement membranes [17]. To determine the sites of activation of MIG-17 and tissue distribution of ... including both the proform and the activated form, was immunoblotted (right lane). Single asterisk, proform of MIG- 17–GFP; double asterisk, activated form of MIG-17–GFP; arrow, profor...

Ngày tải lên: 30/03/2014, 04:20

... exported from the softmax pro software (Molecular Devices) and analyzed by using the software batchki (BioKin Ltd, Pullman, WA, USA). P. Kuzmic et al. Inhibition of lethal factor protease by aminoglycosides FEBS ... existence of multiple enzyme–inhibitor binding sites or the involvement of multiple structural binding modes at the same site. Quantitative analysis of the ionic stren...

Ngày tải lên: 19/02/2014, 06:20

... l M of OsDmc1 in absence of ATP; (2) with 1.25 lM of OsDmc1 in presence of 2.0 m M of AMP-PNP; (3) with 1.25 lM of OsDmc1 in presence of 2.0 mM ATP-c-S; (4) with 1.25 lM of OsDmc1 in presence of ... exchange events where ATP hydrolysis seems to critically decide the rates of the reac- tion system. These studies open up new facets of a plant recombinase func- tion in...

Ngày tải lên: 19/02/2014, 07:20

... exchange rate of RhoA and results in inhibition of lymphocyte and lymphoma cell motility, induction of transcriptional activation by serum response factor and amplification of RhoA transforming potential ... lL of transfection mix (1 lL of SiPort, 75 ng of pRL-TK, 1.25 lg of the luciferase reporter plasmid, and 1 lg of the PRDM1a expression plasmid or control pCDNA3.1 per...

Ngày tải lên: 05/03/2014, 23:20

... mobility of this molecule [84] also sug- gests a high content of extended structure. Figure 2A compares the results of PONDRÒ analysis by two pre- dictors of intrinsic disorder, firstly, a predictor of ... (as esti- mated by a method suggested in [130]). The binding of p27 Kip1 to Cdk2 leading to the inhibition of kinase activity is much slower and is accompanied by the fol...

Ngày tải lên: 07/03/2014, 21:20

... study, detailed molecular events in the pressure-induced cold inactivation of CPY are described. Action of reducing agent which reveals site(s) of cold inactivation The action of reducing agent was ... HCl concentration of 1 M or above. Effect of SDS upon cold inactivation of CPY under high pressure CPY was incubated at )10 °C for 30 min under 400 MPa in the presence of vari...

Ngày tải lên: 08/03/2014, 10:20

... resistant to unfolding and thereby minimize the risk of degradation by the 26S proteasome [3,25]. Given the current lack of knowledge regarding both the stability and folding of the 5 2 -knotted ubiquitin hydrolases, ... and indicative of the population of the hyperfluorescent intermediates decaying as the population of the native state species increased. The folding pathway of...

Ngày tải lên: 16/03/2014, 01:20

... (maximum deviation of 9.3 A ˚ for the Ca atoms of Gly89) and loop L9 (maximum deviation of 4.7 A ˚ at Gly123). The conformational change of loop L9 is evidently caused by the presence of the sodium ... Poland 3 Department of Protein Engineering, Faculty of Biotechnology, University of Wroclaw, Poland 4 Institute of Plant Genetics, Polish Academy of Sciences, Poznan, Pola...

Ngày tải lên: 23/03/2014, 06:20