... were mixed and digested with XbaIandXmaI, purified by phenol/ chloroform extraction and ligated with T4-DNA ligase (Roche, Denmark). E. coli XL-1 cells were transformed by electroporation with the ... IF and the 3¢-untranslated region. This sequence corresponds to a sequence in GenBank accession no. X76562 and encodes a protein of 399 amino acid residues starting with STQTQSS…...
Ngày tải lên: 21/02/2014, 00:20
... snippets with tags and features for training a conditional random field model. At run- time, the model is used to extracting translation candidates for a given term. Preliminary experiments and ... tokens labeled as B and I. Finally, we compute the frequency of all the candidates identified in all snippets, and output the one with the highest frequency. 4 Experiments and...
Ngày tải lên: 19/02/2014, 19:20
Tài liệu Báo cáo khoa học: "WebCAGe – A Web-Harvested Corpus Annotated with GermaNet Senses" docx
... completely manually and is therefore limited to the amount of data that can reasonably be annotated by hand. Leacock et al. (1998), Agirre and Lopez de La- calle (2004), and Mihalcea and Moldovan (1999) propose ... as the gold standard for the sense inven- tory is fully in line with standard practice for English where the Princeton WordNet (Fellbaum, 1998) is typically taken as the...
Ngày tải lên: 22/02/2014, 03:20
Tài liệu Báo cáo khoa học: Human Cdc45 is a proliferation-associated antigen pdf
... normal tissue specimens and tumour entities and compare the Cdc45 signals with the classical proliferation markers PCNA and Ki-67, but also with the novel markers Mcm proteins and geminin. Hopefully, ... [ 35 S]-pulse-chase labelling and IP with Cdc45-specific antibody was performed as described in Experimental procedures. Briefly, HeLa S3 cells were labelled with [ 35 S]-meth...
Ngày tải lên: 19/02/2014, 00:20
Tài liệu Báo cáo khoa học: Human enhancer of rudimentary is a molecular partner of PDIP46/SKAR, a protein interacting with DNA polymerase d and S6K1 and regulating cell growth docx
... pelleted and washed four times with 1 mL of binding buffer and twice with 1 mL of NaCl ⁄ P i and boiled in sample loading buffer. Protein samples were resolved on a 15% SDS ⁄ polyacrylamide gel and ... spectrometry A protein band was excised from a gel and analyzed by tandem mass spectrometry at Laboratory of Mass Spectr- ometry, Institute of Biochemistry and Biophysics PAS, W...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khoa học: "Unsupervized Word Segmentation: the case for Mandarin Chinese" doc
... also be considered to deal with unambiguous cases of numbers and dates in Chinese script. From h → (x 0 n ) and h → (x 0 n−1 ) on the one hand, and from h ← (x 0 n ) and h ← (x 1 n ) we estimate ... Branching Entropy. We improve on (Jin and Tanaka-Ishii, 2006) by adding normalization and viterbi- decoding. This enable us to remove most of the thresholds and parameters from the...
Ngày tải lên: 19/02/2014, 19:20
Tài liệu Báo cáo khoa học: Allosteric modulation of myristate and Mn(III)heme binding to human serum albumin Optical and NMR spectroscopy characterization pptx
... transport of lipophilic compounds and drugs and of medium and long chain fatty acids; among them, myristic acid is a stereotypic ligand to investigate fatty acid binding and transport properties of ... point toward the interface between domains I and III and are stabilized by salt bridges with His146 and Lys190 residues [6,8]. HSA undergoes pH- and allosteric effector-depend...
Ngày tải lên: 20/02/2014, 02:22
Tài liệu Báo cáo khoa học: "Unsupervised Discourse Segmentation of Documents with Inherently Parallel Structure" pdf
... our model is as follows: • Draw the document-level topic proportions β (doc) ∼ GEM(α (doc) ). • Choose the document-level language model φ (doc) i ∼ Dir(γ (doc) ) for i ∈ {1, 2, . . .}. • Draw ... and i ∈ {1, 2, . . .}. • For each part k and each sentence n: – Draw type t (k) n ∼ Unif (Doc, P art). – If (t (k) n = Doc) ; draw topic z (k) n ∼ β (doc) ; gen- erate words x (k) n ∼...
Ngày tải lên: 20/02/2014, 04:20
Tài liệu Báo cáo khoa học: "Joint Word Segmentation and POS Tagging using a Single Perceptron" docx
... Daum ´ e III and Marcu, 2005; Finkel et al., 2006) and for specific problems such as language modeling and utterance classifica- tion (Saraclar and Roark, 2005) and labeling and chunking (Shimizu and Haas, ... tag t with word w 2 tag bigram t 1 t 2 3 tag trigram t 1 t 2 t 3 4 tag t followed by word w 5 word w followed by tag t 6 word w with tag t and previous character c 7 w...
Ngày tải lên: 20/02/2014, 09:20
Tài liệu Báo cáo khoa học: "Chinese Word Segmentation without Using Lexicon and Hand-crafted Training Data" pdf
... statistical and rule-based, exploited two kinds of important resources, i.e., lexicon and hand-crafted linguistic resources(manually segmented and tagged corpus, knowledge for unknown words, and linguistic ... segmenting Chinese texts without making use of any lexicon and hand-crafted linguistic resource. The statistical data required by the algorithm, that is, mutual in...
Ngày tải lên: 20/02/2014, 18:20