... long-chain saturatedfatty acids (SFAs) led to increased expression of proin-flammatory cytokines, inhibition of insulin signaling,induction of endoplasmic reticulum (ER) stress, andpromotion of ... elevations in theexpression of activating transcription factor 4 (ATF4)and of CCAAT⁄ enhancer-binding protein homologousprotein (CHOP) proteins downstream of eIF2a wereobserved (Fig. 4A), indicating ... Hoechst 33342(30 min, 37 °C). Annexin V–fluorescein isothiocyanate(FITC) (BD Pharmigen, San Diego, CA, USA) binding andPI staining were performed according to [44]. Followingincubation with 1.0...