... overexpression approach of RpCAbr and RpCAtr, we cannot know the functionaleffect of changes of some key amino-acids.Origin and number of nonvertebrate CAsAlthough the bootstrap values of the deep branches ... 0.5 lL of BSA (10 mgÆmL)1),1 lL of total RNA (1.24 lgÆlL)1), 2.5 lL of dNTP (4 mmtotal), 1.5 lL of Random Primer 9 (Ozyme, St-Quentin-en-Yvelines, France) (100 ngÆlL)1), 3 lL of diethylpyro-carbonate. ... branchial plumecDNA with an open reading frame of 726 nucleotides and 5¢- and 3 ¢ UTR sequences of 171 and 442 nucleo-tides, respectively. Positions of the primers on the com-plete cDNA are given...