Báo cáo khoa học: Exo-mode of action of cellobiohydrolase Cel48C from Paenibacillus sp BP-23 A unique type of cellulase among Bacillales ppt
Báo cáo khoa học: The twin-arginine translocation (Tat) systems from Bacillus subtilis display a conserved mode of complex organization and similar substrate recognition requirements doc
... (CCAAACCACGTTTACTCACCTCAGCAGCCA ATACCG) for KR mutation; RKDmsAF (GCTGCTGAG GTGAGTCGCAAAGGTTTGGTAAAAACG) and RKD- msAR (CGTTTTTACCAAACCTTTGCGACTCACCTC AGCAGC) for RK mutation; and KRtoKKDmsAF (GCTGAGGTGAGTAAAAAGGGTTTGGTAAAAACG ACAGCG) ... KRtoKKDmsAF (GCTGAGGTGAGTAAAAAGGGTTTGGTAAAAACG ACAGCG) and KRtoKKDmsAR (CGCTGTCGTTT TTACCAAACCCTTTTTACTCACCTCAGC) for KK mutation. SDS/PAGE and western blot...
Ngày tải lên: 16/03/2014, 04:20
Báo cáo khoa học: Structural and functional roles for b-strand 7 in the a-crystallin domain of p26, a polydisperse small heat shock protein from Artemia franciscana pdf
... 5¢-CACGTACAAAGAGAACGTCGACGACG-3¢ 5¢-CGTCGTCGACGTTCTCTTTGTACGTG-3¢ R11 4A 5¢-GAGAATTTCGAGCACGATACAGACTCCC-3¢ 5¢-GGGAGTCTGTATCGTGCTCGAAATTCTC-3¢ Y116D 5¢-CGACGACGAGACAGACTCCCAGAACATGTC-3¢ 5¢-GACATGTTCTGGGAGTCTGTCTCGTCGTCG-3¢ Y. Sun et al. ... as sense and antisense, respectively, for each mutation. p26 mutation Primer R110G 5¢-GGACACGTACAAGGAGAATTTCGACGACG-3¢ 5¢-CGTCGTCGAAATTCTCCTTGTACGTGTCC-3...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: "You Can’t Beat Frequency (Unless You Use Linguistic Knowledge) – A Qualitative Evaluation of Association Measures for Collocation and Term Extraction" pot
... Chapter of the Association for Com- putational Linguistics, pages 188–195. Toulouse, France, July 9-11, 2001. San Francisco, CA: Mor- gan Kaufmann. Katerina T. Frantzi, Sophia Ananiadou, and Hideki Mima. ... University Language & Information Engineering (JULIE) Lab D-07743 Jena, Germany {wermter|hahn}@coling-uni-jena.de Abstract In the past years, a number of lexical association mea...
Ngày tải lên: 31/03/2014, 01:20
Báo cáo khoa học: Exo-mode of action of cellobiohydrolase Cel48C from Paenibacillus sp. BP-23 A unique type of cellulase among Bacillales ppt
... Exo-mode of action of cellobiohydrolase Cel48C from Paenibacillus sp. BP-23 A unique type of cellulase among Bacillales Marta M. Sa ´ nchez, F. I. Javier Pastor and Pilar Diaz Department of ... expres- sion and purification. The encoded enzyme, a cellulase of 1091 amino acids with a deduced molecular mass of 118 kDa and a pI of 4.85, displayed...
Ngày tải lên: 08/03/2014, 02:21
Tài liệu Báo cáo khoa học: Parvalbumin deficiency in fast-twitch muscles leads to increased ‘slow-twitch type’ mitochondria, but does not affect the expression of fiber specific proteins doc
... GGG AAG CTC ACT GG GTG AGG GAG ATG CTC AGT GTT GG 429 RT-PCR CaN AGTAACAATTTTCAGTGCTCCAAAC AATATACGGTTCATGGCAATACTGT 205 RT-PCR CaMKII CTACCCCGGCGCTGGAGTCAAC TCAGATGTTTTGCCACAAAGAGGTGCCTCCT 530 ... calculated from the gene bank databases. For quantitative RT-PCR analyses, images of CaN, CaMKII and GAPDH amplicons from the same sample were acquired with a charge-coupled device (CCD) ca...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: N-Methyl-L-amino acid dehydrogenase from Pseudomonas putida A novel member of an unusual NAD(P)-dependent oxidoreductase superfamily ppt
... were of analytical grade from Nacalai Tesque (Kyoto, Japan) and Wako Pure Chemical Industries (Osaka, Japan). Culture and screening of bacteria Bacterial strains were cultivated for 15–20 h at ... mm each primer (5¢-GGAAT TCCATATGTCCGCACCTTCCACCAGCACCG-3¢ and 5¢-GGGAAGCTTTCAGCCAAGCAGCTCTTTCAGG-3¢), 2.5 U LA Taq DNA polymerase, and 115 ng genomic DNA from P. putida ATCC12633: preincuba...
Ngày tải lên: 19/02/2014, 16:20
Báo cáo khoa học: Arabidopsis thaliana CYP77A4 is the first cytochrome P450 able to catalyze the epoxidation of free fatty acids in plants potx
... protein (512 amino acids) has a calcu- lated mass of 58 134 Da and a pI of 8.71. Enzymatic characterization of CYP7 7A4 was carried out employ- ing microsomes from the yeast strain WAT11 trans- formed ... physi- ological role of CYP7 7A4 . This lack of data could explain the small amount of information available today concerning the ability of plant enzymes to gener- ate e...
Ngày tải lên: 07/03/2014, 03:20
Báo cáo khoa học: Two L-amino acid oxidase isoenzymes from Russell’s viper (Daboia russelli russelli) venom with different mechanisms of inhibition by substrate analogs pdf
... a ligand to enter and anchor at the respective functional sites of LAAO that may facilitate the design of suicidal inhibitors. Abbreviations LAAO, L-amino acid oxidase; NAT, N-acetyltryptophan; ... buffer with a linear flow rate of 12 mLÆh )1 , and 1.5 mL fractions were collected. The LAAO activity of each fraction was assayed using the coupled assay system, and the pH of each fr...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Isoquinoline-1,3,4-trione and its derivatives attenuate b-amyloid-induced apoptosis of neuronal cells pdf
... MO, USA). Caspase peptide substrates Ac-DEVD-pNA, Ac-DEVD-AMC, N-acetyl- Val-Asp-Val-Ala-Asp-p-nitroanilide (Ac-VDVAD-pNA), N-acetyl-Val-Glu-Ala-Asp-p-nitroanilide (Ac-VEAD-pNA), and N-acetyl-Leu-Glu-His-Asp-p-nitroanilide ... 5 Table 2. Selectivity of isoquinoline-1,3,4-trione and derivatives on caspases [IC 50 (lM)]. Data from compounds 1, 2, 3 and 7 is from [30]. Caspase 2 Caspase-3...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Hemitoxin, the first potassium channel toxin from the venom of the Iranian scorpion Hemiscorpius lepturus ppt
... and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402. 48 Sali A & Blundell T (1993) Comparative protein model- ing by satisfaction of spatial restraints. ... code Iv5 6A [25]) was from He. spinnifer; OcKTx1–5 were from O. carinatus [23]; IsTx was from Op. madagascarensis [24]; anuroctoxin was from A. phaiodactylus [26]; and...
Ngày tải lên: 16/03/2014, 06:20