... phospholipid and membrane binding of theother members of the PEBP family. Consequently, thebinding characteristics of PEBP proteins, includingthose of the binding of ICto phospholipid membranes,are ... cytoplasmic inhibitor of vacuolar CPY in the yeast Saccharomyces cerevisiae[1–3]. Recent biochemical and mutational studies of IC[4–8] and the crystal structure of the complex of ICwith CPY (IC–CPY) ... membranes.ResultsMembrane-binding properties of ICIn an attempt to detect and characterize the membranebinding of IC, a member of the PEBP family, we firstperformed a liposome-binding assay of this inhibitorfor...