Báo cáo khoa học: Molecular characterization of H2O2-forming NADH oxidases from Archaeoglobus fulgidus potx

... amino-acid sequence of the artemin monomer consists of 230 residues with a calculated molecular mass of 25 976 Da. The ferritin cDNA (accession number AY062897) of 725 bp consists of an ORF of 516 bp, ... Canada; 2 Department of Molecular Cell Biology, Sylvius Laboratory, Leiden, the Netherlands; 3 Section of Molecular and Cellular Biology, University of California, Da...

Ngày tải lên: 23/03/2014, 20:22

... (A) Scheme of the three-hybrid strategy used in the screen. (B) Number of colonies identified in each step of the screen. (C) Distribution of the 63 distinct sequences in relation of their position ... 3¢ UTR. In recent years, and as a result of great efforts aiming to understand the mechanism of RNA control in animals, the function of a diverse number of RNA-binding proteins...

Ngày tải lên: 18/02/2014, 06:20

... Munn 2,3 and Hongyuan Yang 1 1 Department of Biochemistry, Faculty of Medicine, National University of Singapore, Republic of Singapore 2 Institute of Molecular and Cell Biology, A*STAR Biomedical ... anti-Osh6p serum from cell extracts of wildtype cells, which was absent from extracts of OSH6 knockout cells. On the other hand, the amount of Osh6p protein overexpro- duce...

Ngày tải lên: 07/03/2014, 21:20

... that the three NADH oxidases belong to different phylogenetic clusters. NoxA-1 falls within a group of typical NADH oxidases of  49 kDa, including the well-studied NADH oxidases from Enterococcus ... 1). The physiological role of the putative NADH oxidases in A. fulgidus is still enigmatic. Common NADH oxidases of mesophilic origin are assumed to protect the c...

Ngày tải lên: 08/03/2014, 02:21

... predicted molecular mass of 83 kDa. A comparison of this transglutaminase with other members of the gene family revealed an overall sequence identity of 33–41%. A special sequence feature of this ... of a c-carbonyl of histone H2B by esterification, followed by a nucleophilic attack by an e-amino group of Lys residue of histone H4 [38], the fact that the cross-link is forme...

Ngày tải lên: 08/03/2014, 10:20

... a molecular mass of 53 kDa and a pI of 7.5 (Fig. 2A), corresponding to the predicted size of the putative pro- tein calculated from the HlSCP1 amino acid sequence. To detect the localization of ... We report the molecular characterization and possible function of a serine carboxypeptidase (HlSCP1) identified in the midgut of the hard tick Haem- aphysalis longicornis. HlSCP1...

Ngày tải lên: 16/03/2014, 10:20

... using IMAGEQUANT software (Amersham Biosciences). Standards consisted of 5 lL aliquots of serial dilutions of the reaction mixtures, with the moles of phosphate defined using the ATP c oncentration. Division of ... transfer of the c-phosphate from ATP to the 5¢-hydroxyl of Ado, generating AMP and ADP, is one of several enzymes responsible for maintaining steady-state Ado leve...

Ngày tải lên: 16/03/2014, 18:20

... square fit. The heat of dilution was subtracted from the raw data of titration of Rv3619c with Rv3620c. (B) Far-UV CD spectra of Rv3619c, Rv3620c, and the 1 : 1 complex. CD spectra of 5 l M Rv3619c ... is the recipient of research fellowships from the Council of Scientific and Industrial Research (CSIR), New Delhi, India. S. Srivastava is the recipient of project assistantsh...

Ngày tải lên: 22/03/2014, 16:21

... feature of the amino acid sequences at the C-terminus, which are longer than and different from that of MIHs. To obtain the 5¢ region of this cDNA, a set of specific primers was designed from the ... The identity of RT-PCR products was confirmed by DNA sequencing. Fig. 3. Alignment of Pem-GIH with GIH and MIH from other species of crustacean. The deduced amino acid sequence...

Ngày tải lên: 23/03/2014, 07:20

... at a molecular ratio of 1 : 1000, and further tested for the Met-degrading activity on the basis of the detection of a-keto acid after the addi- tion of 2 mm Met (Fig. 3). Approximately 85% of EhMGL2 ... Kobayashi S, Ikegami F, Noji M, Saito K & Takeuchi T (1998) Molecular cloning and characterization of the genes encoding two isoforms of cysteine synthase in the enter...

Ngày tải lên: 07/03/2014, 05:20