Báo cáo khoa học: by nucleotide affinity cleavage: a distinct nucleotide specificity of the C-terminal ATP-binding site potx

... Comparative analysis of the ATP-binding sites of Hsp90 by nucleotide affinity cleavage: a distinct nucleotide specificity of the C-terminal ATP-binding site Csaba So } ti 1 ,A ´ kos Vermes 1 , ... not otherwise indicated, and after an additional incubation of 15 min at 37 °C affinity cleavage was induced by the addi- tion of 0.5 m M FeCl 3 and 30 m M...

Ngày tải lên: 08/03/2014, 02:21

... ON357, 5¢-TGAAACATCACCAACTAAATC TCCAA-3¢; ON358, 5¢-GACGACTCCATTGTTATAGG AAAAGAGT-3¢; ON359, 5 ¢-GCCGCTGGAGAAACAG CAT-3¢; ON360, 5¢-GCCACCCATTCGTCACAATC-3¢; and ON361, 5¢-ATGCAGAAGGGGATCCGG-3¢. Direct ... in the supernatants were quanti- fied by the BC assay quantification kit (Uptima) using BSA as the standard. Equal amounts of protein were prepared for separation by SDS ⁄ PAGE. Im...

Ngày tải lên: 19/02/2014, 18:20

... 90 and 180 kDa, and the appearance of one band at 40 kDa. These results indicate that most of the protein tyrosine phosphorylations take place down- stream of PI3-kinases, Src family kinases and ... were washed with the reaction buffer. Attached platelets were quantified by c-counting of the wells (Cobra TM II auto-gammaÒ;Packard BioScience Company). Alternatively, platelet a...

Ngày tải lên: 08/03/2014, 02:21

... min )1 at 37 °C from the initial rate. The time course of the reactivation of glycerol-inacti- vated holoenzyme by the reactivase in the presence of ATP and Mg 2+ at a molar ratio of the reactivase ... (AdePeCbl) (an inactive coenzyme analog containing the adenine ring in the upper axial ligand; a model of intact coen- zyme, AdoCbl) in the presence of ATP a...

Ngày tải lên: 15/02/2014, 01:20

... 5¢-TCT ATC TCT CGA TGG ATA CAG A- 3¢; reverse, 5¢-AGG ACA GTA GAA TTA GGT CAC T-3¢). Knockdown of Hsp10 5a and Hsp105b The double-stranded RNA targeting Hsp105 (Dharmacon; 5¢-GCA AAU CAC UCA UGC AAA ... Stephanou A, Isenberg DA, Nakajima K & Latchman DS (1999) Signal transducer and activator of transcrip- tion-1 and heat shock factor-1 interact and activate the transcription o...

Ngày tải lên: 18/02/2014, 06:20

... studied as described in Garg et al. [13], whereas the data of WhiB3 and WhiB4 were taken from Alam & Agrawal [14] and Alam et al. [15] respectively. Md. S. Alam et al. Molecular properties of ... polyclonal anti- bodies raised against each of the WhiB proteins. The absorbance obtained in the positive control (where the antibody used was raised against that particular antig...

Ngày tải lên: 18/02/2014, 12:20

... exclusion, whereas phosphorylated EIIA activates adenylate cyclase (CyaA) and leads to an increase in the intra- cellular cyclic AMP (cAMP) level [1]. Mathematical models of catabolite repression ... assuming a steady state of the intra- cellular metabolites. An approach that combines flux balance analysis (FBA) with an ordinary differential equation (o.d.e.) model of the slow ti...

Ngày tải lên: 18/02/2014, 13:20

... coordinated by the carboxyl oxygen atoms of D8, D9, by the carboxamide oxygen of N92, by the hydroxyl oxygen of S39 and by the oxygen atoms of two water molecules, and has an approximate octahedral ... S, Khachatr- yan A, Vyas S, Arrowsmith CH, Clarke S, Edwards A, Joachimiak A et al. (2001) Structure of Thermotoga maritima stationary phase survival protein SurE...

Ngày tải lên: 18/02/2014, 14:20

... elongation factor 2 (EF-2), 5¢-GACATCACCAAGGGTGTGCA-3¢ (forward) and 5¢-TTCAGCACACTGGCATAGAGGC-3¢ (reverse); GAPDH,5¢-CCGAGCCACATCGCTCAGAC-3¢ (forward) and 5¢-GTTGAGGTCAATGAAGGGGTC-3¢ (reverse). The ... and functional properties of RNase and participates in degradation of IL-1b mRNA Danuta Mizgalska 1 , Paulina We˛ grzyn 1 , Krzysztof Murzyn 2 , Aneta Kasza 1 , Aleksander Koj 1 , Jacek...

Ngày tải lên: 18/02/2014, 14:20

... properties and intracellular localization. DAP-kinases (DAPk), Ca 2+ ⁄ calmodulin-regulated and Ser ⁄Thr kinases, activate signaling pathways that lead to cell death through membrane blebbing and autophagic ... discussed by Aaron Ciechanover (Haifa, Israel), who argued that the ubiquitin proteolytic system covers the pathway for elucidating the basic mechanisms that are pivotal for dr...

Ngày tải lên: 19/02/2014, 02:20