Báo cáo khoa học: Investigation of the kinetics and order of tyrosine phosphorylation in the T-cell receptor f chain by the protein tyrosine kinase Lck potx

Tài liệu Báo cáo khoa học: Complete subunit sequences, structure and evolution of the 6 · 6-mer hemocyanin from the common house centipede, Scutigera coleoptrata pptx

... results in >95% pure protein (Fig. 1).Antibodies raised against the hemocyanin fraction showstaining of the two 75 kDa bands in Western blot, but donot recognize any other proteins of the hemolymph.Although ... lost during the evolution of the Spirostreptidae (Fig. 5). The reason for different hemo-cyanin function and thus structure of Scutigera and Spirostreptus may be found in their distinct life styles. ... allow the assignment of the HcA clone to the major sequence of the upper hemocyanin band in the SDS/PAGE gel (Fig. 1).Two nonmatching amino acids at positions 3 (Glu instead of Cys) and 5 (Ala instead...

Báo cáo khoa học: Mycobacterium tuberculosis ClpC1 Characterization and role of the N-terminal domain in its function ppt

... it is of interest to understand the mode of action of theseproteins in M. tuberculosis. In this study, we functionally characterized the ClpC1 protein of M. tuberculosis, and investigated the role ... [3]. Increased expression of heat shockproteins is triggered by a range of stress conditions, and is also induced in both the host and pathogenduring the process of infection [4].Heat shock protein, ... analyze whether M. tuberculosis ClpC1 preventsformation of protein aggregates, the effect of ClpC1on the heat-induced denaturation of luciferase wasFig. 1. Amino acid sequence of M. tuberculosis...

Báo cáo khoa học: Photosynthetic acclimation: State transitions and adjustment of photosystem stoichiometry – functional relationships between short-term and long-term light quality acclimation in plants ppt

... describes the relation of the total leaf area of a plant (one side) to the covered ground area. This can range from 4 to 16for different types of forests, and leaf area index values of 1–5 can be found ... downstream of primary photosynthesis,leading to a re-orientation in the metabolic network of plants by affecting, for example, carbohydate metabo-lism and the synthesis of nucleotides and amino acids[13] ... stoichiome-try adjustment involves changes in the expression of photosystem genes and in the accumulation of Chl a and Chl b [29]. Despite the differences in the timescales of action, the LTR-like state...

Báo cáo khoa học: "Dynamic programming for parsing and estimation of stochastic uniﬁcation-based grammars∗" pot

... is, Ω(y) is the set of parses of a string y and F( y) is the set of features appearing in the parses of y. In the gram-mars of interest here Ω(y) and hence also F( y) areﬁnite.Maxwell and Kaplan’s ... as the value thatmaximises a product of functions or else as the sum of a product of functions, each of which dependson a small subset of the variables X. These are the kinds of quantities for ... that formost strings y, many of the features in F( y) occur in many of the parses Ω(y). This is often the case in natural language, since the same substructure canappear as a component of many...

Báo cáo khoa học: SHP-1 dephosphorylates 3BP2 and potentially downregulates 3BP2-mediated T cell antigen receptor signaling ppt

... initially identiﬁed asan Abl SH3 domain-binding protein of unknown func-tion [25]. Recently, 3BP2 has been shown to interactwith the Syk and ZAP70 proteins of the Syk family of tyrosine kinases. ... activation [35]. In RBL-2H3 mast cells, phosphorylation of Tyr448 of 3BP2creates a binding site for the SH2 domain of Lyn, aSrc family protein tyrosine kinase, and interaction of Lyn with 3BP2 ... resulting in the recruitment and activation of the ZAP70 and Syk PTKs [2,3]. Theseactivated PTKs further induce the tyrosine phosphory-lation of multiple intracellular proteins, including the adapter...

Báo cáo khoa học: Investigation of the kinetics and order of tyrosine phosphorylation in the T-cell receptor f chain by the protein tyrosine kinase Lck potx

... Investigation of the kinetics and order of tyrosine phosphorylation in the T-cell receptor f chain by the protein tyrosine kinase Lck Hazel R. Housden1, Paul J. S. Skipp1, Matthew P. ... restriction to Lck accessing certain tyrosine residues of the protein chain. Our studies have determined the efﬁciency of phosphory-lation of each of the six f ITAM tyrosines by PTK Lck, from which ... phosphorylation by Lck, conﬁrmed tyrosine 1N to be the ﬁrst phosphorylated.Differences arising in the order of phosphorylation of the subsequent tyrosines in the whole cTCRf chain in vitro,relative to the...

Tài liệu Báo cáo khoa học: Investigation and prediction of the severity of p53 mutants using parameters from structural calculations pptx

... This is indicated by the averageactivity of mutants in the central domain of 26% forWAF1 and 34% for MDM2, 71% for NOXA and 61%for p53R2, and around 45% for the other four promot-ers. For some ... additional binding site needed for the WAF1promoter.Prediction of the severity of mutants in otherproteinsAll parameters used for the predictions of p53 couldbe used for any protein with known ... residue 283 is involved in DNA binding. However, residues 272 and 276 are closeto DNA binding. The other four residues are furtheraway, but at the same side of the protein, indicating apossible...

Báo cáo khoa học: Investigation of the contribution of histidine 119 to the conduction of protons through human Nox2 pptx

... to introduce the mutations of His ﬁ Leu for the three histidine residues of interest within the thirdtransmembrane domain of Nox2. Each of the three possiblecombinations of pairs of histidine ... particle and release of its genome into the cell [18,23]. Therefore, the in ux of protons through the M2 protein is important in the life cycle of the virus. The M2 protein has a histidineresidue, ... over come by the addition of arachidonate acid. The site of interaction with and the mechanism by which arachidonate results in the activation of both the NADPH oxidase and the gating of the protonchannel...

Báo cáo khoa học: Investigation of the substrate speciﬁcity of a b-glycosidase from Spodoptera frugiperda using site-directed mutagenesis and bioenergetics analysis pdf

... exclusively from the l ength increase of theseinteractions. This also indicates that the length decrease of the residue 39 side -chain affects /4e and /4a interactionsequally.Replacement of Q39 with ... ratio, the mutation series E ﬁ D ﬁ S may be useful in changing the preference for glucosides and galactosides of other family1 b-glycosidases. The preference for fucosides was also affected by the replacement ... ) indicate that thisinteraction i s not affected by the exchange of a chargedparticipant ( carboxyl group of the E side -chain) for anuncharged participant (amide g roup of the Q side -chain) .Otherwise,...

Báo cáo khoa học: Investigation of the interaction between the atypical agonist c[YpwFG] and MOR docx

... surface of the bind-ing site, and this polarization effect can be obtained by using the QM description of the ligand. Looking at the electronic structure of the ligands in their boundedconformations, ... site, by using the so-called ‘blind docking’approach, a technique introduced for the detection of possible binding sites and modes of binding of peptideligands by searching the entire surface of ... one (the one showing the best docking score).As the lack of ﬂexibility in the protein may in uence the binding modes of the ligands, and the afﬁnities and orientations may vary signiﬁcantly from...

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