Báo cáo khoa học: Studies on structure–function relationships of indolepyruvate decarboxylase from Enterobacter cloacae, a key enzyme of the indole acetic acid pathway ppt
... acid.
Indolepyruvate decarboxylase, the key enzyme in the
biosynthetic pathway of indole- 3 -acetic acid, catalyses the
formation of indole- 3-acetaldehyde and carbon dioxide
from indole- 3-pyruvic acid. The enzyme ... concentration and
independent of the concentration of the auxiliary enzyme,
confirming that the coupled assay monitors the true rat...
... proteins. The relative cross-reactivity of poly-
clonal antibodies was used to probe the conforma-
tional variations at the tertiary level among WhiB
proteins. Polyclonal antibodies against all WhiB ... studied as described in Garg
et al. [13], whereas the data of WhiB3 and WhiB4 were taken from Alam & Agrawal [14] and Alam et al. [15] respectively.
Md. S. Alam et al. Molecu...
... (two each) from the
arginine-rich epitope (217RRRRKR222) of the third intra-
cellular loop were exchanged (D
2
R1: 217AARRKR222,
D
2
R2: 217AAAAKR222, D
2
R3: 217AAAAAA222), as well
as one variant ... influence the parameters, and the fractional
contribution of the additional lifetime was close to
zero. Figure 5 shows the typical time-dependent donor
decays for the D
1
–CFP...
... induce a conforma-
tional transition that modifies the intra-subunit interaction
between the regulatory and the catalytic domains. This
interaction is followed by the formation of a catalytically
activated ... surface plasmon resonance
analysis, and the rate of phosphorylation of Ser16 by cAMP-
dependent protein kinase. Comparison of the inhibitory
properties of the...
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... Under aqueous conditions, deviations
from the allowed regions of the Ramachandran plot
are greater than observed under the other two
conditions, suggesting the incomplete formation of
an a- helical ... high
relaxation with 5-DSA have relatively lower relaxation
because of the presence of Fe
3+
. The apparent fast
relaxation of V7¢ in the presence of both paramagne...
... structure
of the human C-domain in solution, and present data
on its dynamics. On the basis of the structural data,
we have performed a mutational analysis of the
C-domain and investigated the impact of ... motions of the minidomain backbone on the
picosecond–nanosecond time scale are rather small,
as shown by the large values of the order parameter
S
2
,...
... in Table 1 contains a
feature type, feature values, and an experimental
result that will be explained later. Each feature
consists of a type and a value. The features are
basically some attributes ... discuss the contribution of each
feature set and the relationship between
the number of training data and the ac-
curacy.
1
Introduction
Dependency structure analy...
...
conjoining of TR's with the same
Actor
Ex.: An operational amplifier act-
ivates a passive network to form an
active analogue. An operational
amplifier performs mathematical op-
erations. ... (cf. Pi[ha, 1980),
there is the partitive participant (a glass
of water) and the free modifications of
appurtenance (a leg of the table], of gen-
eral relationship (n...
... electrostatic properties at the root of the activa-
tion mechanism, may represent a case of divergent
evolution. Indeed, the HPT b-chain appears to have
evolved from a common ancestor by mutation of only
two ... 1) spanning the
entire length of both a- and b-chains. The availability
of a template that spans the entire length of both
HPT variants and provides...