Báo cáo khoa học: Reaction of human UMP-CMP kinase with natural and analog substrates docx
Tài liệu Báo cáo khoa học: "Demonstration of the UAM CorpusTool for text and image annotation" docx
... Abstract This paper introduced the main features of the UAM CorpusTool, software for human and semi-automatic annotation of text and images. The demonstration will show how to set up ... e.g., MMAX-2 (Müller and Strube 2006), GATE (Cunningham et al 2002), WordFreak (Morton and LaCivita 2003) and Knowtator (Ogren 2006). With the exception of the last of these...
Ngày tải lên: 20/02/2014, 09:20
Tài liệu Báo cáo khoa học: Structure of RNase Sa2 complexes with mononucleotides – new aspects of catalytic reaction and substrate recognition pptx
... complex, O2¢ and O3¢ form hydrogen bonds with OE1 of Glu56 and NE2 of His86, respectively. Arg67 and Arg71 interact with the oxygens of the phosphate group, and site- directed mutagenesis studies ... Preparation command of glide. Interactions of probe atoms with proteins were calculated with the Receptor Grid Generation command of V. Bauerova ´ -Hlinkova ´ et al. S...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt
... containing 4 pmol of each primer, 10 lLof2· SYBR Premix Ex Taq, and 2 of 20 lL single-strand cDNA reactions. To determine the absolute copy number of the target transcripts in indi- vidual cDNA reaction ... exon 7 of fragment C represents a dele- tion of 39 bp. The thick lines indicate intron 6 of 75 bp in fragments B and C, 3¢ portion of 35 bp in intron 5 in fragments C...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khoa học: Unfolding of human proinsulin Intermediates and possible role of its C-peptide in folding/unfolding pptx
... HPI, and to further learn the molecular mechanism of the initiation of HPI folding. Acknowledgements We thank Profs M. A. Weiss and Q X. Hua for providing human proinsulin and helpful ... sequences and in vitro refolding pathway of PIP and HPI. (A) Amino acid sequences of PIP and HPI. Amino acids are shown in the one-letter code. The numbering of the residues in HPI a...
Ngày tải lên: 19/02/2014, 12:20
Báo cáo khoa học: ` Inhibition of human ether a go-go potassium channels by 2+ Ca ⁄calmodulin binding to the cytosolic N- and C-termini potx
... the presence of Ca 2+ with a K D of 327 ± 112 nm; binding of BD-N and BD-C2 were not altered with labeled with Cy5 instead of TMR. In FCS measurements fluorescently labeled peptides of both, the ... in steps of 3) and C-terminal (amino-acids 480–962 in steps of 3 and amino-acids 645–895 in steps of 2) cytosolic domains of hEAG1. Binding of BODIPY FL-labeled hCaM...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Interaction of human stefin B in the prefibrillar oligomeric form with membranes Correlation with cellular toxicity doc
... initiating a cascade of detrimental events for the cell. Interaction of granular aggregates and globular oligo- mers of an amyloidogenic protein, human stefin B, with model lipid mem- branes and monolayers ... channel hypothesis of AD and neurodegeneration in general, is not incompatible with other key elements of toxicity, as, for example, the deregulation of Ca 2+ hom...
Ngày tải lên: 07/03/2014, 17:20
Tài liệu Báo cáo khoa học: Basis of recognition between the NarJ chaperone and the N-terminus of the NarG subunit from Escherichia coli nitrate reductase pdf
... biphasic, with the best fit obtained with a two binding site model, comprising a first site with binding stoichiome- try (n) of 0.3 ± 0.2 and a binding constant (K d )of 3.4 ± 4 · 10 )9 m and a second with ... parameters of the interaction (on rate constant k on and off rate constant k off ) between NarJ and the NarG(1–15) peptide. Taking into account the existence of tw...
Ngày tải lên: 16/02/2014, 14:20
Tài liệu Báo cáo khoa học: Roles of heat shock factors in gametogenesis and development pptx
... over-expres- sion of Hsp60 and Hsp70 and exhibit abnormal mor- phology in correlation with increased proliferation, premature differentiation and elongation, inhibition of denucleation and of loss of organelles ... mechanisms for the lack of HSF2. In line with this hypothesis, double disruption of Hsf1 and Hsf2 is associated with sterility and complete arrest of...
Ngày tải lên: 18/02/2014, 04:20
Tài liệu Báo cáo khoa học: Effect of siRNA terminal mismatches on TRBP and Dicer binding and silencing efficacy pdf
... target, loading of the appropriate guide strand into the RISC is critical. Improved understand- ing of the interactions of siRNAs with TRBP and Dicer will enable improved design of siRNA therapeutics. In ... more sta- ble end of the siRNA duplex and directs binding of Dicer-2 to the other, less stable, end, and hence the guide strand is selected through interaction of it...
Ngày tải lên: 18/02/2014, 06:20
Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
... roles in develop- ment and in health and disease [1]. Their properties have been studied by a combination of in vitro analy- ses of human primary ECs and in vivo analyses of genetically modified ... concentra- tion of G418 (Invitrogen) of 400 lgÆmL )1 . G418-resistant colonies were picked and expanded for PCR genotyping and the formation of embryoid bodies. One ESC clon...
Ngày tải lên: 18/02/2014, 17:20