Báo cáo khoa học: Uptake of bilirubin into HepG2 cells assayed by thermal lens spectroscopy Function of bilitranslocase doc

... Passamonti et al. Bilirubin uptake into HepG2 cells FEBS Journal 272 (2005) 5522–5535 ª 2005 FEBS 5535 Uptake of bilirubin into HepG2 cells assayed by thermal lens spectroscopy Function of bilitranslocase Sabina ... of bilitranslocase controls the per- meability of HepG2 cells to bilirubin. Results and Discussion Expression of bilitransloca...

Ngày tải lên: 07/03/2014, 21:20

... net movement of anandamide into the cells is coupled to the activity of intracellular FAAH [23,24]. FAAH is responsible for the catabolism of anandamide and it contributes to anandamide uptake by making ... space and the interior of the cell. Therefore, in the presence of various inhibitors of FAAH (e.g. phenylmethylsulfonyl fluoride), the uptake is limited by the shift...

Ngày tải lên: 08/03/2014, 08:20

... autophosphorylation activity, loss -of- function (kinase-inactive) forms of the MAPKs were produced as GST-lofMPK3 and GST- lofMPK6. However, when lofMPK3 and lofMPK6 proteins were tested for phosphorylation ... increasing amounts of PTI1-4 enhanced the autophosphorylation activity of OXI1 (Fig. 4B) as well as the transphosphorylation of MBP. Simply by incubating the two proteins...

Ngày tải lên: 14/02/2014, 19:20

... Hospital, Boston, MA, USA 3 Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Japan 4 Laboratory of Biodefense and Regulation, ... were increased in TG mice by the HF diet. mRNA expression of adipogenic genes in WAT of TG mice We measured the amounts of PGD 2 in WAT after 6 weeks of the...

Ngày tải lên: 16/02/2014, 09:20

... activity of factor VIIa by replace- ment of residues 305, 314, 337 and 374: evidence of two unique mutational mechanisms of activity enhancement. Biochem J 379, 497–503. 21 Eigenbrot C, Kirchhofer ... overall functional defect of G372A- FVIIa was, if anything, smaller in the presence of TF, which suggests that the allosteric effect of TF, influenc- ing burial of the N-terminu...

Ngày tải lên: 18/02/2014, 08:20

... these, bombyxins, a family of peptides produced by the brain of the silkmoth Bombyx mori, were the first to be dem- onstrated to have structural homology to vertebrate insulins [7,8]. Bombyxin-II, one of ... structure of 8K-BLP. (A) MALDI-TOF MS analysis of the purified peptide. (B) The N-terminal sequence of 8K-BLP as determined by sequence analysis. X, unidentified residues. (C...

Ngày tải lên: 18/02/2014, 13:20

... ERE2, ERE3 and ERE4 of the HOXC13 promoter. As seen in Fig. 5A, no significant binding of actin was observed in any of the EREs, irrespective of the absence and presence of E2. Binding of ERa and ERb was ... of binding of MLL1 was observed in ERE3, no significant E2-dependent binding of any of the MLLs was observed in ERE3 and ERE4 (Fig. 5A, lanes 5–8). Significant amounts o...

Ngày tải lên: 18/02/2014, 14:20

... on l-dopa as cofactor to eliminate the characteristic lag period of tyrosinases [8,20,21]. Figure 3 shows the rate of TH activity as a function of the concentration of l-dopa cofactor added to ... accumulated by the sponta- neous disproportion of dopaquinone can be titrated at different periods of time by addition of sodium perio- date. According to the high preference of...

Ngày tải lên: 19/02/2014, 07:20

... Murakami 2 and Kenji Aoki 2 1 Division of Science of Biological Resources, Graduate School of Science and Technology, 2 Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, ... acid was synthesized by the methods of He and Spain [5]. 2-Hydroxymuconic 6-semi- aldehyde was prepared by incubating catechol with resting cells of a mutant, strain Y-2,...

Ngày tải lên: 19/02/2014, 16:20

... sign) of a cycle is the product of the values (the signs) of its steps. The sign o f the magnitude of a Ôminus combined autoinfluence path of order pÕ equal those of the arithmetic product of its Ó ... absolute magnitude of a combined autoinfluence path is the product of all the rates divided by the product of all the concentrations of its species. Therewith the c oeffici...

Ngày tải lên: 19/02/2014, 16:20