Báo cáo khoa học: Identifying remote protein homologs by network propagation ppt

... 272 (2005) 5119–5128 ª 2005 FEBS Identifying protein homologs by network propagation W. S. Noble et al. MINIREVIEW Identifying remote protein homologs by network propagation William S. Noble 1 , ... level of the hier- archy, contains protein domains that are presumed to be homologous to one another, whereas protein Identifying protein homologs by network...

Ngày tải lên: 07/03/2014, 21:20

... Identifying Topic and Focus by an Automatic Procedure Eva Haji~ov~ & Petr Sgall Institute of Formal and ... index f, and its subject belongs to the topic, which we denote by the index t; (bb) indef'mite, then the subject is (indexed by) f and the verb is t. In either case, the other complementations ... less equivalently, a flat structure in a constituency based gramm...

Ngày tải lên: 18/03/2014, 02:20

... to remove two co-purifying proteins that could be identi- fied by Edman sequencing, MS and a database search as the ribosomal protein S15 and the ferric uptake reg- ulation protein from E. coli (Swiss-Prot ... first approach, the protein was expressed in inclusion bodies followed by refolding from guanidine– HCl using conventional Ni-affinity chromatography. In a second approach, the...

Ngày tải lên: 30/03/2014, 04:20

... reactions can be modulated either by tight binding to proteins, establishing a functionally static RNA protein complex, or by transient interac- tions with proteins that dissociate from the RNA ... confor- mation is recognized by the protein, interacts with it to form a stable complex, and is thereby removed from the conformational equilibrium [23]. The second mechanistic class of pr...

Ngày tải lên: 14/02/2014, 19:20

... interaction) l MPK6 binds to PTI1-4 by pull down (View interaction) l PTI1-4 and MPK3 phosphorylate by protein kinase assay (View interaction) l OXI1 phosphorylates OXI1 by protein kinase assay (View ... HIS-OXI1 and GST-PTI1-4 proteins or HIS-PTI1-4 and GST-OXI1 proteins, the GST-tagged proteins were pulled down with glutathi- one-agarose beads. The proteins were then detected by...

Ngày tải lên: 14/02/2014, 19:20

... organization of HIV gag -protein shell in virus-like particles produced by recombinant baculovirus. Virol 198, 288–296. 9 Campbell S & Vogt VM (1995) Self-assembly in vitro of purified CA-NC proteins from ... assembly in vitro by inositol phos- phates. Proc Natl Acad Sci USA 98, 10875–10879. 12 Mayo K, Huseby D, McDermott J, Arvidson B, Finlay L & Barklis E (2003) Retrovirus capsi...

Ngày tải lên: 18/02/2014, 06:20

... Author Journal compilation ª 2009 FEBS Gag polyprotein, yields the matrix protein (MA), the capsid protein (CA), the nucleocapsid protein (NC) and p6 proteins, as well as two spacer pep- tides. Currently, ... (p50) and RNase H (p15). Conversely, cleavage of the Keywords CTD protein; HIV; NMR; organic molecules; protein peptide interactions; protein protein interactions; structure; X...

Ngày tải lên: 18/02/2014, 06:20

... concentrations of proteins were established by measuring A 280 . After purification, the puri- ties of all mouse recombinant protein C preparations were determined by SDS-PAGE followed by silver staining. Preparation ... vesicles by this method is approximately 110 ± 25 nm. Liposomes were stored at 4 °C and were used within 1 week of production. Measurement of protein membrane inter...

Ngày tải lên: 18/02/2014, 06:20

... Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 298, 1911–1912. 18 Morrison DK & Davis RJ (2003) Regulation of MAP kinase signaling modules by scaffold proteins ... controlled by both protein kinases and protein phosphatases [22–24]. Various types of stimuli activate JNK through phosphorylation by the dual-specificity kinase...

Ngày tải lên: 18/02/2014, 13:20

... PIAS, protein inhibitor of activated STAT proteins. Site Mechanism Example Extracellular space Modification of ligand-receptor affinity ) usually by binding to ligand dimers Dimeric secreted proteins ... counter-regulated (e.g. by STAT3 and Nanog). Recapitulating, the BMP-signaling pathway promotes pluripotency only indirectly by driving expression of ID genes in a Smad1-dependent manne...

Ngày tải lên: 19/02/2014, 00:20