Báo cáo khoa học: Light-harvesting complex II protein CP29 binds to photosystem I of Chlamydomonas reinhardtii under State 2 conditions doc

... 4797–4806 ª 20 05 FEBS Light-harvesting complex II protein CP29 binds to photosystem I of Chlamydomonas reinhardtii under State 2 conditions Joanna Kargul1, Maria V. Turkina 2 , Jon Nield1, Sam ... immobilized metal afﬁnity chromatography; LHCII, light-harvesting complex II; PSI, photosystem I; PSII, photosystem II; S1 and S2, State 1 and State 2. FEBS Journal 27 2 (20 05) 4797–4806 ª 20 05 FEBS 4797Although ... themobile light-harvesting complex II (LHCII) antenna [2 5]. The phosphorylated LHCII is proposed to transfer physically from PSII to PSI to balance energydistribution between, and optimize the...

Báo cáo khoa học: Tyrosine nitration in the human leucocyte antigen-Gbinding domain of the Ig-like transcript 2 protein ppt

... glucocorticoid receptor, wherenitration leads to an increase in binding capacity [25 ].Identiﬁcation of nitration site in the extracellulardomain of ILT2In the extracellular domain of ILT2, there ... binding domain of ILT2could undergo nitration, which implies conformationalchanges.ILT2 nitration increases HLA-G binding To determine whether treatment with NO modiﬁes theinteraction of ILT2 ... the inhibition of NKL cytotoxicity againstFig. 2. (A) Amino acid sequence coverage and sites of nitration of SIN-1-treated rhILT2-Fc, obtained by LC-MS ⁄ MS analysis. Protein wasnitrated with...

Tài liệu Báo cáo khoa học: Transient DNA ⁄ RNA-protein interactions docx

... association–dis-sociation–reassociation or via direct transfer (kdt). For the sake of clarity, some of the species participating in the individual equilibriaare omitted.Fig. 1. Structure of ... combinato-rial regulation of transcription [18].Electrostatics plays a driving role in transient pro-teinÆnucleic acid interactions, as well as in selectingand stabilizing the speciﬁc ones. Indeed, ... how a nonhistone protein recognizes and binds to the nucleosome(Fig. 4). It was found that arginines of the switchbackloop of RCC1 interact with an acidic patch on thehistone H2A–H2B dimer, whereas...

Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf

... Uekita T, Gotoh I, Kinoshita T, Itoh Y, Sato H,Shiomi T, Okada Y & Seiki M (20 04) Membrane-type 1matrix metalloproteinase cytoplasmic tail-binding protein- 1 is a new member of the Cupin ... T, Itoh Y, Yana I, Ohno H & Seiki M (20 01)Cytoplasmic tail-dependent internalization of membrane-type 1 matrix metalloproteinase is importantfor its invasion-promoting activity. J Cell Biol ... Selection of bbHT1080 cells expressing EGFP-furin by FACS.Fig. S5. Furin expression impairs activation of pro-MMP2.Fig. S6. Fusion of GM130 coiled-coil region to MT1-MMP ICD increased its binding...

Tài liệu Báo cáo khoa học: The yeast ubiquitin ligase Rsp5 downregulates the alpha subunit of nascent polypeptide-associated complex Egd2 under stress conditions docx

... ubiquitin ligasessuch as CHIP, San1 and Ubr1 are involved in ubiquiti-nation of denatured proteins under stress conditions [ 32 34]. In addition, identiﬁcation of ubiquitinatedforms of mis-folded ... RING-type ubiquitinligase, under physiological conditions such as glucosedepletion [24 ]. In addition, Egd2 does not associatewith a ubiquitin molecule, although it contains aubiquitin-associated ... model protein, suggesting that Egd2 has a chaperone-like activity [44].Ubiquitination of Egd2 by Rsp5 under stress conditions H. Hiraishi et al. 529 2 FEBS Journal 27 6 (20 09) 528 7– 529 7 ª 20 09...

Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx

... alsoeliminated in cells incubated with 20 lm H89, aninhibitor of PKA but also of other kinases [28 ], for 25 min prior to treatment with 10 lm serotonin(Fig. 3B). The serotonin-induced ERK1 ⁄ 2 ... (Fig. 4A). Inter-estingly, neither inhibiting phosphorylation of Ser916by mutating the amino acid to alanine, nor poten-tially mimicking it by mutation to aspartic acid orglutamic acid, inﬂuenced ... phosphory-lation was concomitantly reduced, as expected based onthe results of our previous publication [24 ]. Cotrans-fection of HEK293 cells with the PKA inhibitor protein kinase inhibitor, in addition...

Tài liệu Báo cáo khoa học: Long-distance interactions between enhancers and promoters The case of the Abd-B domain of the Drosophila bithorax complex pdf

... targeted to the proximal promoter, in Type II, it is restricted to the distal promoter (Fig. 4, bot-tom), and ﬁnally, in Type III, the boundary retains itsenhancer-blocking activity and no targeting ... the iab regions to the Abd-B pro-moter. This assumption prompted a detailed analysis of the tmr, which led to the identiﬁcation of a set of different cis-acting elements within it [19] (see Fig. ... transgenic studies. Uponincorporation into the transcriptionally inactive precur-sor cells of the germ line, a protein complex may beformed irreversibly on the transgenic DNA, this DNA- protein complex...

Tài liệu Báo cáo khoa học: The diacylglycerol and protein kinase C pathways are not involved in insulin signalling in primary rat hepatocytes doc

... selective PKC inhibitor bis-indolylmaleimide I, which predominantly inhibits conven-tional and novel isoforms, i. e. the a-, d-ande-isoforms(Fig. 6). Owing to its isoform speciﬁcity, the inhibitorcompletely ... insulin signal(Fig. 6). Selective inhibition of PKCd by the inhibitorrottlerin (5–10 lM) was also without effect on insulinsignalling (data not shown).Finally, we tried to inhibit insulin signalling ... immunoprecipitated enzyme, which agreeswith the inability of insulin to translocate PKCf.Inhibitor studiesStimulation of glycogen synthesis by insulin could not beinhibited by the relatively selective...

Báo cáo khoa học: The SWI⁄SNF protein BAF60b is ubiquitinated through a signalling process involving Rac GTPase and the RING ﬁnger protein Unkempt doc

... attached to otherubiquitins in a highly processive reaction to form apolyubiquitin chain [ 12, 13]. The speciﬁcity of protein ubiquitination is conferred by E3 ligases, which havethe ability to bind ... FEBSRING ﬁnger proteins devoid of intrinsic ubiquitinligase activity have been found to form oligomericcomplexes, especially with other RING ﬁnger proteins,resulting in active E3 ligases [19 22 ].We ... e1487.15 Pickart CM (20 04) Back to the future with ubiquitin.Cell 116, 181–190.16 Kirkin V & Dikic I (20 07) Role of ubiquitin- andUbl-binding proteins in cell signaling. Curr Opin CellBiol...

Báo cáo khoa học: Tetracysteine-tagged prion protein allows discrimination between the native and converted forms pptx

... [14].Rigid spacing between both arsenic atoms in FlAsHenables it to bind with high afﬁnity and speciﬁcity to theTC motif introduced into a variety of different proteins[15]. The binding of FlAsH ... labeling of recombinant proteins in live cells. Anal Chem 73, 29 20– 29 28.33 Martin BR, Giepmans BN, Adams SR & Tsien RY (20 05) Mammalian cell-based optimization of thebiarsenical-binding ... ectopic expression of mutant prion proteins. J Biol Chem 28 2, 187 02 18710.38 Vorberg I, Groschup MH, Pfaff E & Priola SA (20 03)Multiple amino acid residues within the rabbit prionprotein...

Xem thêm