Báo cáo khoa học: Assignment of the [4Fe-4S] clusters of Ech hydrogenase from Methanosarcina barkeri to individual subunits via the characterization of site-directed mutants pdf

... 4741–4753 ª 2005 FEBS 4753 Assignment of the [4Fe-4S] clusters of Ech hydrogenase from Methanosarcina barkeri to individual subunits via the characterization of site-directed mutants Lucia Forzi 1 ,Ju ¨ rgen ... directly below EchF. In general the purity of the enzyme from these mutants was lower than the enzyme isolated from the EchF2...

Ngày tải lên: 07/03/2014, 21:20

... of the CA atom of Ala82 ⁄ 82¢.P1¢-Nle showed three conformations for the side chain and had closer contacts with the CB atom of Ala82 in PR V82A than observed for Val in the PR. The CE atom of ... and the side chain of Lys45¢. Furthermore, P3 is Arg in CA-p2 and Thr in p2-NC. As a result, the carbonyl oxygen of P3 Arg interacted with the amide of Asp29 instead o...

Ngày tải lên: 30/03/2014, 20:20

... terminal in the sinus gland, as a result of late and progressive isomerization of the Phe3 of the CHH during the migration of the secretion vesicles along the axonal tract [39,41]. The immunohistochemical ... unambiguously the presence of the d-Trp in VIH II (not shown). Characterization of cell types in relation to CHH and VIH isomers To study the distri...

Ngày tải lên: 18/02/2014, 11:20

... understanding of the enzymes involved in the detoxification of phytoalexins, includ- ing their substrate specificity as well as the molecular mechanisms of detoxification, is crucial. To this end, we ... described above for the BH assay, except that the temperature ranged from 5 to 56 °C. The activation energy (E a ) was determined from the slope ()E a ⁄ R) of Arrhe...

Ngày tải lên: 18/02/2014, 14:20

... was observed in the cytoplasm of the cells. The staining appears to be specific of the probe sequence because the staining is clear with the complementary sequence to RpCAbr, but not with the sense ... mRNA in both the epidermal cytoplasm of the branchial filaments and in the cytoplasm of the trophosomal bacteriocytes. We could only detect RpCAbr mRNA in the...

Ngày tải lên: 18/02/2014, 16:20

... hydrodynamic radius of 1.98 nm for the TTF-1 HD under the condi- tions of this study, i.e. very close to the mean radius of the NMR structure of the molecule (1.94 nm) [4]. The conclusions inferred ... inertia. In the context of RSDM, the detailed analysis of the three spectral densities J(0), J(x N ) and J(0.87x H ) allowed us to obtain a rather complete des...

Ngày tải lên: 18/02/2014, 16:20

... binding isotherm was fitted to the data, expressed in terms of the heat change per mole of ligand against the ligand to macromolecule ratio. From the binding isotherm values for the reaction stoichiometry, ... The Authors Journal compilation ª 2006 FEBS 4251 from the 3D structures [12,14]. Therefore, the differ- ences in the far UV CD must be due to the contri...

Ngày tải lên: 19/02/2014, 06:20

... 23S.5DG b pre-RNA, and the binding of a minimum of n Mg 2+ ions leads to formation of the active complex, whereas binding of a minimum of m Mn 2+ ions forms the inactive complex. The sizes of the active ... the large P6 extension and the last three nucleotides (AU XG) of the intron. The sizes of the intron (InDG b ) and 5¢ exon (5E) are indicated. The a...

Ngày tải lên: 19/02/2014, 07:20

... FEBS 5723 endothelial growth factors (VEGF) and the family is therefore often referred to as the PDGF ⁄ VEGF family. The PDGF family of growth factors The PDGF family consists of PDGF-A, -B, ... candidates. Receptor binding of PDGFs The PDGFs bind to the protein tyrosine kinase receptors PDGF receptor-a and -b. These two recep- tor isoforms dimerize upon binding the PD...

Ngày tải lên: 19/02/2014, 07:20

... the origin of the kingdom Plantae among the phyla of the ancestral kingdom Protozo a [45]. Hence, it is reasonable to suppose a common origin of extant APXs in the ancient line of protists. The ... early i n the diversification of this clade. The remaining APXs can b e divided into two distin ct subbranches. The subbranch of cytosolic APXs currently only has mem...

Ngày tải lên: 19/02/2014, 16:20