Báo cáo khoa học: Molecular characterization of Osh6p, an oxysterol binding protein homolog in the yeast Saccharomyces cerevisiae pot

... ORD, OSBP related domain; ORP, oxysterol binding protein related protein; OSBP, oxysterol binding protein; OSH, yeast gene encoding oxysterol binding protein; Osh6p, yeast OSBP homolog; PA, phosphatidic ... of Osh6p, an oxysterol binding protein homolog in the yeast Saccharomyces cerevisiae Penghua Wang 1 , Wei Duan 1 , Alan L. Munn 2,3 and Hong...

Ngày tải lên: 07/03/2014, 21:20

... therefore predict that various PUF of A. thalina may be involved in many different processes in the plant. RNA -binding capacity of the APUM proteins pfam analysis of all putative APUM proteins ... RNA -binding proteins has been elucidated [1–4]. Despite this, translational control through the binding of RNA -binding proteins to 3¢ UTR tran- scripts has been poorly desc...

Ngày tải lên: 18/02/2014, 06:20

... and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein- dye binding. Anal. Biochem. 72, 248–254. 19. Weiss, R. (1970) The solubility of ... remained in the Nox assay. The decrease in NADH in the first assay was related to the amount of H 2 O 2 found in the second assay. Protein was determined acco...

Ngày tải lên: 08/03/2014, 02:21

... a component of the signal-transducing G protein complex [7]. The cDNA of G ha , involved in the transmission of adrenergic stimuli, is identical to that of tissue TG of human endothelial cells ... : 26 of the original volume using Centricon-10 (Amicon). Proteins were determined by the modified method of alkaline copper (Lowry) protein assay [23] using BSA as the...

Ngày tải lên: 08/03/2014, 10:20

... disease-causing agents in humans and animals by injecting their saliva, which contains anticoagulants and other bioactive com- ponents as well as pathogens, into the blood pool dur- ing feeding [16]. Suppression of ... anti- HlSCP1 serum. An immunoreactive band was detected in the fraction containing membranes and membrane organelles (Fig. 3A). To determine the endogenous form...

Ngày tải lên: 16/03/2014, 10:20

... over the other. For instance in the spleen, the short isoform is the predominant AK species , whereas in the t estis and kidney, the long isoform is more abundant. Most brain regions, with the exception ... than the cerebellum and various components of the forebrain. Furthermore, one or the o ther AK isoform predominates in most tissues, including the brain, wher...

Ngày tải lên: 16/03/2014, 18:20

... single-site binding model for determination of thermodynamic parame- ters. The parameters used in fitting were the stoichiom- etry of association (n), the binding constant (K b ), and the change ... results, combined with those of previous studies, suggest that unfolding of the proteins is required for dissociation of the complex and membrane binding. In the pr...

Ngày tải lên: 22/03/2014, 16:21

... site is underlined. The num- bers on the left and right of the sequences show the coordinate of nucleotides and amino acids in corresponding lines. P. monodon GIH cDNA and its role in Vg expression ... that in control shrimp. The increase in the ratio of Vg to actin transcripts in the GIH-depleted background suggested that functional knockdown of Pem-GIH led to...

Ngày tải lên: 23/03/2014, 07:20

... underlining indicates helical regions, and residues in blue indicate b structures in Artemia ferritin and artemin. Residues in the di-iron ferroxidase centers of humanferritinHchainandinArtemia ... region of the protein, neither the N-terminus nor C-terminus of artemin has similarity to any known protein. Protein Predict indicates a secondary structure for Artemia ferrit...

Ngày tải lên: 23/03/2014, 20:22

... reflects the binding process, as the apolar residues of the N-domain are buried predominantly because of intermolecular interactions (binding) and not because of intramolecular interactions (folding). ... November 2006) doi:10.1111/j.1742-4658.2006.05579.x Chemokines elicit their function by binding receptors of the G -protein- cou- pled receptor class, and the N-terminal...

Ngày tải lên: 19/02/2014, 05:20