Báo cáo khoa học: The inﬂuence of cold shock proteins on transcription and translation studied in cell-free model systems pdf

... are based on the binding of the CSPs to DNA and RNA. However,other possible mechanisms are the inhibition of transcription and ⁄ or translation by interaction with proteins involved in this process ... inhi-bition of a non -cold shock mRNA can be combinedwith the results of [3]. They found an increase in the translation of cold shock mRNAs and of cold tolerantmRNAs with the addition of CspA to a translation assay. ... single-stranded DNA as present during transcription and consequently block the transcription process. The effect of cold shock proteins on translation As shown above CSP inhibits transcription...

Báo cáo khoa học: "The clinical value of daily routine chest radiographs in a mixed medical–surgical intensive care unit is low"

... interpreta-tion of the data and were involved in drafting the manuscript.MG participated in analysis and interpretation of the data and in drafting the manuscript. PS, JS and MV contributed to the conception ... the conception and design of the study and manuscript revision.JK was involved in the design and statistical analysis of the study. MS conceived and coordinated the study and wasinvolved in the interpretation ... obtain CXRs after insertion of endotra-cheal tubes, intravenous lines and chest drains, but not afterinsertion of nasogastric tubes. In addition, CXRs wereobtained in the case of worsening of...

Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf

... homologs of Caenor-habditis elegans synMuvB proteins and is composed of p130, E2F4 ⁄ 5 and DP1 ⁄ 2 and a module containing the MuvB proteins Lin-37, Lin-52, Lin-54 and chromatin-associated Lin-9 and ... function of Lin-9 in repression. This indicates that Lin-9 is involved in the activation of these genes in cycling cells. In summary, it has been shown that the DREAMcomplex controls expression of ... binding these factors.Consistent with a function of p107 and p130 in the control of CDE ⁄ CHR promoters is the loss of regula-tion observed for the expression of cyclin A, B-Myb and Cdc2 in...

Tài liệu Báo cáo khoa học: The crystal structure of human a-amino-b-carboxymuconatee-semialdehyde decarboxylase in complex with 1,3-dihydroxyacetonephosphate suggests a regulatory link between NAD synthesis and glycolysis ppt

... conformations of the Trp–Met couple can beobserved; the arrows indicate the unidirectional movement of the two protein residues upon ligand binding. The dotted line highlights the hydrogen bond ... A˚resolutionrange. The solution of the rotation function was usedto perform the translation function calculations in both the P3221 and P3121 space groups. A clear solu-tion was obtained for the ... Phe294-Pro295-Leu296 amino acid stretch (Fig. 3A,B). The ligand binds in an extended conformation, with itsphosphate moiety located in the proximity of the zincion and with the hydroxymethylene group pointingtoward...

Tài liệu Báo cáo khoa học: "The Eﬀect of Corpus Size in Combining Supervised and Unsupervised Training for Disambiguation" pdf

... Proceedings of the COLING/ACL 2006 Main Conference Poster Sessions, pages 25–32,Sydney, July 2006.c2006 Association for Computational Linguistics The Eﬀect of Corpus Size in Combining Supervised ... that the combined sys-tem only improves the performance of the parser f or small training sets. Sur-prisingly, the size of the unannotatedcorpus has little eﬀect due to the noisi-ness of the ... the subject and V the verb. So the supremum of that lattice is SV:pMN and the in mum is 0:p (which in this case correspondsto the MI of verb attachment and occurrence of the preposition).LBD is...

Tài liệu Báo cáo khoa học: The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol pptx

... band upon the addition of component A (Fig. 2D). Three bands were observed in the Ôcomponent BÕ fraction upon nondenaturing PAGE. Posi-tions of the top and middle bands coincided well with the two ... cobalamins (2.9–5.1°)[9,10].Figure 4C indicates the comparison of the position of the a-acetamide side chain of pyrrole ring A of the corrin ring in the glycerol dehydratase-bound cobalamin (Fig. ... appeared uponprotein staining, and only the upper one stained upon activitystaining (data not shown). The Mrvalues for the upper and lower bands were 220 000 and 200 000, respectively. Thesedata...

Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx

... the amino group of the main chain at Glu268. The oxygen of the main chain at Asp266 was bound to amino groups of main chain of Tyr269 and Ala270 by hydrogen bonds. The interactions should maintain ... ﬁll in the gap between surrounding residues of the depression, and the side chain of the mutated residue might restrain the mobility of the N-terminal region on the surface byinteraction with the ... maintain the structure of the C-terminalregion. In the wild-type enzyme, no bonds were observedbetween the N-terminal region and the C-terminal region on the molecular surface, and the two regions...

Báo cáo khoa học: The ATPase activities of sulfonylurea receptor 2A and sulfonylurea receptor 2B are inﬂuenced by the C-terminal 42 amino acids doc

... Walker A and Walker B motifs of one NBD and the linker domain of the other. In the absence of Mg2+, there is little difference in ATP block of Kir6.2 ⁄ SUR2A and Kir6.2 ⁄ SUR2Bchannels [5], indicating ... consisting of maltose-binding protein(MBP) linked at its C-terminus to one of the NBDs of SUR2 (MBP-NBD fusion proteins) . Figure 1B,C showsSDS/PAGE analysis of puriﬁed full-length SUR2A and SUR2B. ... equation using a Km of 460 lM,aVmax of 2.52 nmol PiÆminÆmg)1 and an offset of 0.1 nmol PiÆminÆmg)1forSUR2A, and a Km of 41 lM,aVmax of 0.73nmol PiÆminÆmg)1 and an offset of 0.05...

Báo cáo khoa học: Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebraﬁsh (Danio rerio) pptx

... lacks intron II, and the fabp1bgene from zebraﬁsh, which contains an additionalintron in the 5¢-untranslated region [6]. Each of the intron ⁄ exon splice junctions in the zebraﬁsh fabp6 geneconform ... sections revealed intense hybridization signals in the adrenal homolog of the kidney and the distal region of the intes-tine, and to a lesser extent in ovary and liver, a transcript distributionthat ... bywhole-mount in situ hybridization. fabp6 transcripts were ﬁrstdetected at 72 h postfertilization in the distal region of the intestine(A) and remained conﬁned to this region of the intestine up...

Báo cáo khoa học: The pH dependence of kinetic isotope effects in monoamine oxidase A indicates stabilization of the neutral amine in the enzyme–substrate complex ppt

... perturbation of the substrate amine pKa on binding to the enzyme has been observedwith other amine oxidases, and likely identiﬁes a common mechanism forincreasing the effective concentration of the ... deuteration) and to observe a corresponding shift in the macroscopic ionization. Deuteration of the atoms bonded to the amine nitrogen is known to causean increase in the amine pKa; in part ... effectively increasing the concentration of the neutral amine species. We do not propose that it isonly the protonated form that initially binds, butrather that preferential binding of the deprotonatedform...

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