Báo cáo khoa học: Characterization and cDNA cloning of a clofibrate-inducible microsomal epoxide hydrolase in Drosophila melanogaster potx
Báo cáo khoa học: Characterization and cDNA cloning of a clofibrate-inducible microsomal epoxide hydrolase in Drosophila melanogaster potx
... low at the beginning of the last larval stadium, but
it gradually increased, reaching a peak at the wandering
stage late in the last larval stadium. At the prepupal stage,
EH activity declined ... clofibrate and harvested at 5, 8
and 14 h post-treatment. The poly (A) -RNA was extracted from the
larvae, and 300 ng mRNA of each sample was loaded on a gel. Actin
mRNA served as an...
Tài liệu Báo cáo khoa học: Characterization and functional expression of cDNAs encoding thyrotropin-releasing hormone receptor from Xenopus laevis Identification of a novel subtype of thyrotropin-releasing hormone receptor ppt
... (5¢-ATAATGGATAA
CGTAACTTTTGCTG-3¢)/TRHR1-4 antisense (5¢-TC
TGTTAAATGTACCTAAGTAGGCA-3¢)andTRHR2-2
sense (5¢-CAGCAAAATGGAAAATAGTAGC-3¢)/
TRHR2-4 antisense (5¢-CGACACTGTAGTAG-AGAT
CACC-3¢), respectively. The PCR ... three sets of primers: first set, A5 1 (5¢-
GATGTCACGCAGAGTGAGCAGGTAG-3¢)/TRHR-7
(5¢-GAGACCATACAGAAC-C-3¢); second set, A5 2 (5¢-
AGAGTGAGCAGGTAGCGAGAGGAG-3¢)/TRHR-8
(5¢-GGGGGTGT...
Tài liệu Báo cáo khoa học: Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima doc
... ferulic acid, methyl or
acetyl groups, and depolymerases. The latter can be
classified into lyases (b-elimination) and hydrolases [2].
All hydrolases involved in degradation of pectin are
classified as ... members of family 28 of the glycoside
hydrolases, including the endopolygalacturonases, exo-
polygalacturonases and rhamnogalacturonases [3,4].
Although a handful of endopol...
Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt
... development and use of
N-acylsulfonamides and sulfonimides as antagonists of nucleic acid-binding
proteins.
Database
Structural data for the two RNase A complexes are available in the Protein Data Bank ... Functional and structural analyses of N-acylsulfonamide-
linked dinucleoside inhibitors of RNase A
Nethaji Thiyagarajan
1
, Bryan D. Smith
2,
*, Ronald T. Raines
2,3
an...
Tài liệu Báo cáo khoa học: Active and regulatory sites of cytosolic 5¢-nucleotidase doc
... GCATCAACTTTCAAAAGAT
F127E CGAGATAAGGGGACCAG TTAAATCCATGTGCACAG
R144E AGAAGATGACACTGAAAG TGAATAAATTTATTTGGATAC
I152D CGATCTGAACACACTATTC TAAAATCTTTCAGTGTCAT
N154D GGACACACTATTCAACCT AGAATGTAAAATCTTTCAGT
K31 1A ... AAGAAAGTAACTGACGACATGGACATGTG CACATGTCCATGTCGTCAGTTACTTTCTT
Y55G AGTGTTTTGGGTTTGACATGGATGGCACACTTGCTG CAGCAAGTGTGCCATCCATGTCAAACCCAAAACACT
T56V AGTGTTTTGGGTTTGACATGGATTATGTGCTTGCTG...
Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... Canyuk B, Focia PJ & Eakin AE (2001) The role for
an invariant aspartic acid in hypoxanthine phospho-
ribosyltransferases is examined using saturation muta-
genesis, functional analysis, and ... respectively, indicating that Asp137 functions as
a catalytic base [12]. However, a tight binding of N7
of the purine base and Asp137 was observed in HPRT
in complex with ImmGP,...
Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of flavoproteins: electron transfer through the nitric oxide synthase flavoprotein domain pdf
... C-terminal flavopro-
tein or reductase domain that binds NADPH, FAD
and FMN. The two domains are separated by a
CaM-binding motif. During catalysis, NADPH-
derived electrons transfer into the FAD and ... attached
flavoprotein and heme domains of NOS are also an
unusual feature shared by only a handful of prokary-
otic CYP proteins [4,8,25].
In comparison, the NOS flavoprotein dom...
Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of flavoproteins: probes of hydrogen tunnelling pptx
... Using a combination of
experimental and computational approaches, we have
shown that H-transfer reactions can occur by ‘deep’
tunnelling and the reaction can be enhanced by local-
ized dynamics ... (R,S)-[4,4-
2
H
2
]-NADPH can be pre-
pared in the same manner as (R,S)-[4,4-
2
H
2
]-NADH.
However, an NADPH-specific enzyme such as PETNR
must be used in place of MR. NADH
4
and NA...
Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of flavoproteins: photosynthetic electron transfer from photosystem I to NADP+ doc
... subunits,
PsaA and PsaB, via a loop that also plays a role in the
attachment of PsaC [12]. PsaC, PsaD and PsaE are
located at the cytosolic site (Fig. 1A) [2,7,13–16]. PsaC
carries the terminal F
A
and ... loop that is maintained by
a small two-stranded antiparallel b sheet at the top
(Fig. 1B). The NADP
+
-binding domain includes resi-
dues 139–303 and consists of a c...
Tài liệu Báo cáo khoa học: Structural and functional studies on a mesophilic stationary phase survival protein (Sur E) from Salmonella typhimurium ppt
... enterica
Typhimurum strain IFO12529 genomic DNA as the template
using Deep Vent DNA polymerase (New England Biolabs,
Ipswich, MA, USA), a sense primer (CATATGGCTAGC
ATGCGCATATTGCTGAGTAAC) containing ... S, Khachatr-
yan A, Vyas S, Arrowsmith CH, Clarke S, Edwards A,
Joachimiak A et al. (2001) Structure of Thermotoga
maritima stationary phase survival protein SurE: a
novel acid phos...