Báo cáo khoa học: Protein stabilization by compatible solutes Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR docx

... Protein stabilization by compatible solutes Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR Pedro Lamosa 1 , David L. ... protein known to date [27]. In this context, assessing the changes in the dynamic behaviour of proteins in the presence of solutes is expected to shed light on the stab...

Ngày tải lên: 07/03/2014, 21:20

... FEBS Absorption spectra of the complexes of heme and HOs resemble the spectrum of myoglobin with one exception, the HO of Drosophila melanogaster. In the HO of fruit fly, the iron of heme was not ... 3. Absorption spectra of various forms of the Syn HO-2–heme complex. The concentration of the complex was 7 lm. The spectra are the iron(III) (solid line), ir...

Ngày tải lên: 30/03/2014, 15:20

... characterization of the purified enzyme The c oncentrated enzyme solution (fraction 6) was yellow i n color. The enzyme s olution showed one main absorption peak at 266 nm and a broad absorption band in the ... identified by GC-MS analysis, and the determination of released ammonia. The coupled enzyme assay revealed the mechanism of the deamination reaction and the sub...

Ngày tải lên: 19/02/2014, 16:20

... at the end of the titration, even after addition of excess Cu(I), some species still retained Zn(II) ions. The discussion of the Cu(I) coordination behavior becomes more complex in view of the ... homonuclear Cu 5 preparations. Under these conditions, low Zn contents Table 4. Analytical characterization of the recombinant preparations of the Cu complexes yielded by...

Ngày tải lên: 07/03/2014, 02:20

... tem- perature. Influence of interference The influence of the main constituents of plasma on the determination of 2.00 · 10 )6 molÆL )1 GSH were studied. The criterion for interference was fixed at a ± 5.0% variation ... bis(b-CD)s. Because of the conformation of the linker of 1, the nitrogen atoms and amido bond formed two chelate rings to coordinate with copper (...

Ngày tải lên: 07/03/2014, 05:20

... models. The first replaces the unigram component of LDA topic models with multi-word se- quences or collocations generated by an AG. The second extension builds on the first one to learn aspects of the ... the Doc and Doc  nonterminals — but it has the advantage that the LDA distributions map straight-forwardly onto PCFG nonterminals). The terminals W of the CFG c...

Ngày tải lên: 23/03/2014, 16:20

... metabolism. The functional association was further aided by integration of protein interaction data with data on gene expression, localization, func- tion, evolutionary conservation, protein structure ... than 70% of the promoters are bound by at least one of the top 10% most highly connected TFs. In addition, 82% of the promoters are bound by at least one of th...

Ngày tải lên: 30/03/2014, 09:20

... (Milan Theater) and is not at the beginning of that constituent. Finally, the syntactic depen- dence trace of a word is the path of IOB values from the root of the tree to the word itself. The last ... measurement – the relative position of the two words in their respective sen- tences. The relative position is defined to be the word’s absolute position divided...

Ngày tải lên: 31/03/2014, 01:20

... The assays were incubated for 4 h and were stopped by the addition of 0.5 M EDTA pH 8 to a final concentration of 20 m M . The incorporated 14 C was measured by liquid scintillation counting of ... rubber transferase activity at the final concentration of 10% (v/v) in the rubber transferase assay, so these were the chosen conditions for conducting the inhibitor studies...

Ngày tải lên: 21/02/2014, 00:20

... upper). Higher concentrations of fatty acids accelerated the formation of the LDs, but the toxic effects of fatty acids on the cells became evident. Therefore, the cells cultured under normal conditions ... [14], and detected the changes in distribution of the labeled protein before and after the formation of the LDs. To complete pulse-labeling of the Halo-...

Ngày tải lên: 07/03/2014, 05:20