Báo cáo khoa học: The calpain 1–a-actinin interaction Resting complex between the calcium-dependant protease and its target in cytoskeleton doc

... a-actinin, either in direct contact or in a complex including the two proteins. In conclusion, our study proves the interaction between a-actinin and calpain 1 and locates binding motifs within regions ... to the origin of interactions [9,10,12,27] with the targets, the stability of complexes in the resting stage [3,48] and the activation state [7,33] of...

Ngày tải lên: 07/03/2014, 21:20

... form part of the copper binding sites in this protein. Although there are four histidines in the C-terminal, another histidine is present at position 18, and given the folded state of the protein it ... performed and no binding was observed. In our hands, as others [54], the most reproducible data was obtained from injections of the metal into a solution of the protein...

Ngày tải lên: 19/02/2014, 06:20

... discarded and the pellet containing the [H 3 ]PAF bound to the denaturated BSA is dissolved in the scintillation cocktail (dioxane-base) and the radioactivity was determined by liquid scintillation ... entered the analyzer through an interface plate and a 100-mm orifice, while the declus- tering potential was maintained between 60 and 100 V to control the collisiona...

Ngày tải lên: 17/03/2014, 03:20

... direct interaction with the protease, characterized by a lack of interaction between r 32 and CIII (Fig. 4). As for CII, competition between r 32 and CIII for binding to HflB would also decide the ... effective inhibitor. We also found that there is no interaction between CIII and r 32 . From these results, we suggest that the inhibition of r 32 by CIII is due to a di...

Ngày tải lên: 23/03/2014, 10:20

... t M .As for t M , the intrinsic signal is faster than the extrinsic signal, however, the timing of the combined signal is between the intrinsic and the extrinsic signal, which results in S i <1. S K < ... t M , and s) are smaller for the extrin- sic stimulus (a) than for the intrinsic stimulus (b). Contrary to Table 3, the response to the combined signal is...

Ngày tải lên: 30/03/2014, 10:20

... ABSTRACT Existing models of plan inference (PI) in conversation have as- sumed that the agent whose plan is being inferred (the actor) and the agent drawing the inference (the observer) have ... transparent from the query in (11) that Q intends to talk to Kathy, intends to find out how to stand on his head, and intends his doing the latter to play a role in his plan...

Ngày tải lên: 31/03/2014, 17:20

... DS (1999) Interactions con- trolling the membrane binding of basic protein domains: phenylalanine and the attachment of the myristoylated alanine-rich C-kinase substrate protein to interfaces. Biochemistry ... are insufficient in the case of the Y11L-NAP-22- peptide to modulate the sequestering of cholesterol. In the case of NAP-22, the combined interactions of the myri...

Ngày tải lên: 07/03/2014, 17:20

... that benomyl binds to tubulin at a site that is distinct from the colchicine and vinbastine binding sites in tubulin [10,11]. Although benomyl inhibits mitosis in mamma- lian cells, its antimitotic ... significantly decreased the distance between the sister kinetochore pairs in metaphase cells and increased the level of the checkpoint protein BubR1 at the kinetochore...

Ngày tải lên: 30/03/2014, 10:20

... rearrangement of the protein during the docking to the binding site on the ribosome or other ligands. Strikingly, the second most flexible part of the protein (if one does not take into account the N-termi- nal ... binding of the protein to the ribosome. The second, and more plausible, is that the loop is situated at the interface between the M and N domains of...

Ngày tải lên: 07/03/2014, 05:20

... 1785 protein bound to the agarose. This binding was con- firmed by the observation that only a trace of the pro- tein was left in the unbound fraction. Pre-incubation of the recombinant protein with ... observed and the initial GS activity was maintained (116.5 ± 7.3% of the initial activity). The protective effect was abolished when the recom- binant protein was heat-ina...

Ngày tải lên: 07/03/2014, 17:20