Báo cáo khoa học: Two separate regions essential for nuclear import of the hnRNP D nucleocytoplasmic shuttling sequence ppt

... are important for the nuclear import mediated by Trn-1. Results Determination of hnRNP D NLS The exon 8 of hnRNP D encodes the 21 amino acids sequence common to all the isoforms D0 1, D0 2, D1 , and ... and a primer set of a 5¢ EcoRI-attached primer designed for the defined N-terminal amino acid sequence of NLS and a 3¢ SalI-attached primer designed fo...

Ngày tải lên: 07/03/2014, 21:20

... Only the amino-terminal sequence of the AF1376 gene product Fig. 2. Room temperature reduced/oxidized difference spectrum of the purified HmeCD from A. profundus. The spectrum of the reduced enzyme ... 1.5-mL cuvette to record the oxidized spectrum. Solid sodium dithionite was then added (2–5 mg) and several successive spectra of the reduced pyridine hemochromes were rec...

Ngày tải lên: 30/03/2014, 13:20

... cytosolic DA represents an additional risk of oxidative stress, as DA can auto-oxidize at the neutral pH of the cytosol and thereby form toxic DA-quinone species, superoxide radicals and hydrogen ... showed the presence of a strong broad band centered around 75 kDa in the DAT-expressing cells that was absent in the mock-transfected cells. The broad band is probably due to t...

Ngày tải lên: 14/02/2014, 21:20

... a1 and a3 of KIX (Fig. 2B) [33]. When the MLL1 TAD peptide is added to the binary complex, the TAD peptide adopts a helical conformation in which the conserved residues of MLL1 TAD (residues 2845–2853) ... ternary complex, the histone H3 peptide binds in a deep chan- nel that divides a pair of acidic lobes, one of which is composed of residues from the SET-I region and...

Ngày tải lên: 16/02/2014, 14:20

... oxidative damage. Specific antioxidants include superoxide dismutase for superox- ide, catalase for hydrogen peroxide, and glutathione peroxidase for hydrogen peroxide and lipid peroxide. In addition, ... is important to note that the synthesis of MT1 and MT2 in the injured or diseased brain is quite different from the synthesis of GIF. While GIF synthesis is gen- erally consid...

Ngày tải lên: 16/02/2014, 15:20

... Grb10 and Grb14 in the regulation of IR degradation [5]. Additional studies are thus needed to determine whether Grb14 is involved in insulin-induced endocy- tosis and ⁄ or degradation of the IR. In ... them. Structural studies of the kinase domain of the IR in complex with the PIR–BPS of Grb14 and of the Grb14 SH2 domain have allowed us to propose a model for the...

Ngày tải lên: 18/02/2014, 18:20

... the Materials and methods. Completion of the hydrolysis was confirmed by the disappearance of the (Met1)-ONC (M23L) signal in the MALDI-TOF mass spectra 1 h after the addition of AAP. The pH was adjusted to ... under the conditions of this study. On the other hand, mass peaks corresponding to the removal of additional residues were not detected, indicating that t...

Ngày tải lên: 19/02/2014, 12:20

... to eliminate the need for developmental data sets, the available data were partitioned only into training and test sets, with one additional hour set aside for code development and debugging. It ... utterances in the manual and ASR transcripts of training data, and then extracting the mismatched word sequences, anchored by matching words. The matching words serve as contexts...

Ngày tải lên: 20/02/2014, 07:20

... have used backoff models for combining in-domain and 42 out -of- domain data for the purpose of bootstrap- ping a part -of- speech tagger for Chinese, outper- forming standard methods such as EM. 4 Backoff ... 2004) for phrase extraction and decoding. Word alignment is performed in both directions using the IBM- 4 model. Phrases are then extracted from the word alignments usin...

Ngày tải lên: 22/02/2014, 02:20

... at the middle of the molecule. Tryptic digestion of CI at the above two regions yielded two extremely folded structures or domains [designated N-terminal domain (NTD) and C-terminal domain (CTD)] ... resi- dues involved in the dimerization of LexA CTD were also present in the CTD of /11 CI (data not shown). The CTDs of two /11 CI monomers may therefore be respons...

Ngày tải lên: 07/03/2014, 00:20