Báo cáo khoa học: Gene expression in response to endoplasmic reticulum stress in Arabidopsis thaliana ppt

... protein response genes in Arabidopsis S. Kamauchi et al.3476 FEBS Journal 272 (2005) 3461–3476 ª 2005 FEBS Gene expression in response to endoplasmic reticulum stress in Arabidopsis thaliana Shinya ... G13(cAMP -response- element-binding protein-related pro-tein) gene product related to activating transcriptionfactor 6 as a transcriptional activator of the mammalianunfolded protein response. ... misfolded proteins in the ER (ER stress) . To solve this problem, eukaryoticcells sense ER stress and induce a set of genes calledunfolded protein response (UPR) genes. In the bud-ding yeast Saccharomyces...

Báo cáo khoa học: Gene expression silencing with ‘speciﬁc’ small interfering RNA goes beyond speciﬁcity – a study of key parameters to take into account in the onset of small interfering RNA off-target effects potx

... potential cytotoxic effects of siRNAs,which can lead to the silencing of unintended mRNAs. In this study, weused a low-density microarray to assess gene expression modiﬁcations in response to ﬁve ... cytosolic proteins such asdsRNA-dependent protein kinase and ⁄ or membranereceptors such as Toll-like receptor 3 (TLR3), leading to a general repression of translation [11]. An in am-matory ... 2008 FEBS 2753 Gene expression silencing with ‘speciﬁc’ small interferingRNA goes beyond speciﬁcity – a study of key parameters to take into account in the onset of small interfering RNAoff-target...

Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

... accumulation in HepG2 cellscultured in iron-rich media To examine changes in iron metabolism parameters weexamined expression of the transferrin receptor, ferritinand storage iron in HepG2 cells ... in iron-rich mediashowed a further increase in ROS production as deter-mined by an increase in DCFH mean ﬂuorescenceintensity (Fig. 2B, thick line). In addition, determin-ation of acrolein ... transferrin receptor (thick lines) in nonpermeabilized cells cultured without and with iron. Thin linesrepresent background staining with mouse Igs. (B) Histogramsshow background staining with...

Tài liệu Báo cáo khoa học: "Using Automatically Transcribed Dialogs to Learn User Models in a Spoken Dialog System" doc

... Dialogs to Learn User Models in a SpokenDialog SystemUmar SyedDepartment of Computer SciencePrinceton UniversityPrinceton, NJ 08540, USAusyed@cs.princeton.eduJason D. WilliamsShannon LaboratoryAT&T ... does not change during the call, which allowsus to group many user actions together into genericplaceholders e.g. At= FirstNameLastName.After doing this, there were a total of 13 possiblevalues ... Figure 1. In this way, we were able to create synthetic training sets of varying sizes, as wellas a test set of 1000 dialogs. Each generated dialogd in each training/test set consisted of a sequence...

Tài liệu Báo cáo khoa học: Functional expression and mutational analysis of ﬂavonol synthase from Citrus unshiu pptx

... arginine residue (Arg287 in Citrus FLS) can beascribed to 2-oxoglutarate-binding [7,23]. This arginine isalso conserved in IPNS and ACC oxidase with slightlydifferent functionalities, binding ... substitution of glycine by alanine andproline, respectively, or of proline by glycine (Table 1). Eachindividual mutation was veriﬁed by DNA sequencing usingthe dideoxynucleotide chain-termination method ... conserved amino-acid residues which includetwo histidines (His221, His277) and one acidic amino acid(Asp223) residue for FeII-coordination, an arginine (Arg287)proposed to bind 2-oxoglutarate,...

Báo cáo khoa học: "Using Conditional Random Fields to Predict Pitch Accents in Conversational Speech" pptx

... CRF:All in Table5). We get about 0.5% increase in accuracy, 76.1%with a window of size w = 1.Using larger windows resulted in minor increases in the performance of the model, as summarized in Table ... by aseries of intonational events: accents and bound-aries. Labelers were instructed to use duration, am-plitude, pausing information, and changes in f0 to identify events. In general, labelers ... ourselves to 1-orderMarkov model features to encode inter-label de-pendencies. The information used to encode theobservation-label dependencies is explained in de-tail in Section 4. In CRFs,...

Báo cáo khoa học: "A multi-staged approach to identifying complex events in textual data" ppt

... route we have taken towards this end is to model the domain of corporate finance throughan interactive suite of language processing tools.Maytag, our prototype, makes the followingnovel contribution. ... as tailored to the financial do-main, and some forward-looking exten-sions to the approach that enables users to specify classifications on the fly.1 IntroductionOur goal is to support the ... Corporation202 Burlington Rd.Bedford, MA 01730 USAcontact: mbv@mitre.org (Vilain)AbstractWe present a novel application of NLPand text mining to the analysis of finan-cial documents. In particular,...

Tài liệu Báo cáo khoa học: Use of hydrostatic pressure to produce ‘native’ monomers of yeast enolase ppt

... occur during e xposure to pressure. The ﬁrstparameter to change D2, shows a clear d ependence uponprotein concentration, indicating that D2 is monitoringdissociation of t he protein into monomers. ... hydrophobicthan the interior. In addition, several polar amino acids,especially arginine, lysine, glutamine and glutamate arefound more frequently at the interface than in the interior.The degree ... on protein concentration, indicating that eno-lase is dissociating into monomers. The small changes in theUV spectrum a nd the retention of activity lead to a model in which enolase, in t he...

Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

... suggesting thatthe heterologous expression of genes encoding metallo-proteins can lead to incorrect metal ion incorporation.This is observed in rubredoxin and desulforedoxinswhere zinc, instead ... L-tryptophan-containingstandards were used to simulate the protein background. In certain puriﬁcation protocols, iron was determinedspectroscopically from triplicates by the phenantrolinemethod ... metallocofactor containing small subunit (R2F) of theCorynebacterium ammoniagenes ribonucleotide reductase was reintroducedinto strain C. ammoniagenes ATCC 6872. Efﬁcient homologous expression from...

Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf

... obtainedusing the Cdc2 and Cdc25C promoters. In G0, proteins appear to bind to the CDE ⁄ CHR, as monitored by in vivo footprinting. Accord-ing to these early experiments all binding is lost in G2⁄ ... functionMKLP1 ⁄ KIF23 mitotic kinesin-likeprotein 1 ⁄ kinesinfamily member 23Kinesin-like protein, motor enzyme that moves antiparallel microtubules, localizes to the interzone of mitotic spindlesPLK ... cooperation was conﬁrmed by Lin-54 bind-ing to the CHR, cooperating with E2F4 binding to theCDE in EMSAs in vitro [86].ChIP experiments on the Cdc2 gene also providedinsights into the association of...

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