Báo cáo khoa học: Origins of DNA replication in the three domains of life pdf

... and 1L8Q (DnaA). The AAA+ domains of both proteinsare shown in cyan with ADP indicated in red. The helix-turn-helix(HTH)-containing DNA binding domain of DnaA is in green and the winged helix ... seen in Fig. 4, both proteins possessN-terminal AAA+ domains and C-terminal DNA binding domains (DBDs). In DnaA, the DBD containsa helix-turn-helix; in the archaeal proteins, the DBDhas a winged ... origin of replication in the archaea[52]. Interestingly, in a situation reminiscent of that in several bacteria where their origin is adjacent to the gene for the initiator, DnaA, the single...

Báo cáo khoa học: Nanoparticles can induce changes in the intracellular metabolism of lipids without compromising cellular viability docx

... HIF-1ais involved directly in the accumulation of lipids trig-gered by QDs under the conditions of uncompromisedcell survival in the presence of serum.On the basis of the results obtained in this ... storage in LDs. The PI3K ⁄ Akt signaling pathwaystimulates lipogenesis via SREBP-1. FAS is the enzyme responsiblefor the synthesis of palmitate, the precursor of FFAs. The inhibition of signaling ... possibility; however, the inhibition of PI3K with LY294002 and the inhibition of FASwith cerulenin caused the disappearance of dropletsand prevented the formation of new droplets duringexposure to...

Báo cáo khoa học: C ﬁ G base mutations in the CArG box of c-fos serum response element alter its bending ﬂexibility Consequences for core-SRF recognition potx

... provide information on the localrepartition of the angles involved in the SREfoshelixbending.Relative effect of bending strainThere are several indications of a redistribution of the strains ... Indeed, the change in the array of the hydrogen bonds at thymine of SREGGfosis probably a sign of perturbation in the hydration scheme along the minor groove of the (A ⁄ T)domain. The G–C ... doublemutations affect binding of the proximal core-SRFsubunit to the ﬁrst half-site of the CArG box. As amatter of fact, the structure of the (A ⁄ T) domain of the three oligonucleotides is...

Báo cáo khoa học: Key substrate recognition residues in the active site of a plant cytochrome P450, CYP73A1 ppt

... Mutation of all arginines led to a signiﬁcant loss in protein stabilitysuggesting that they are involved in protein fold structurerather than binding of the substrate. The overall picture of the CYP73A1 ... affect the binding of cinna-mate and the coupling of the hydroxylation reaction [9].This effect was probably due to a direct interaction of itsside-chain with the aromatic ring of the substrate. ... experiments [10]. The results of the NMRanalysis indicated that the average initial orientation of the substrates in the catalytic site of the resting Fe(III)protein is roughly parallel to the haem....

Báo cáo khoa học: A novel phosphorylated glycoprotein in the shell matrix of the oyster Crassostrea nippona pptx

... 1866–1871 of the 1.9 kb cDNA. The relevantFig. 4. Nucleotide sequence of the 1.9 kbcDNA and deduced amino acid sequence.Numbers on the left indicate the nucleotidepositions in the 1.9 kb cDNA sequence(upper) ... greenstaining. Arrows on the right side of the lanes indicate the position of the 52 kDa component. A weakly stained band in lane A doesnot correspond to the 52 kDa component but a minor componentwith ... sequence of DCGVDCGYYEPV (score of 19) at the N-terminal region of the deduced 44 kDaprotein and an additional sequence of DNNGDGNG(score of 16) in the NG repeat sequence at the C-ter-minal region...

Tài liệu Báo cáo khoa học: Ionic strength and magnesium affect the speciﬁcity of Escherichia coli and human 8-oxoguanine-DNA glycosylases pdf

... address the in uence of the reaction conditions on the binding of Fpg and OGG1to damaged DNA, we determined Kdvalues for bindingunder the same conditions as used for the kinetic experi-ments. In ... incorporation of 8-oxoG into DNA during replication [7–9]. Inacti-vation of the GO system increases the mutagenesis rate in bacteria [11] and increases the risk of cancer devel-opment in mouse models ... from the B -DNA to the Z -DNA form [58]. Thus, the apparent increase in the C ⁄ A speci-ﬁcity of Fpg and OGG1 in the presence of MgCl2maybe caused by a shift in the conformational equilibriumof...

Báo cáo khoa học: Akt-dependent phosphorylation negatively regulates the transcriptional activity of dHAND by inhibiting the DNA binding activity pdf

... dHAND, we next examinewhether the DNA binding activity of dHAND could beaffected by Akt phosphorylation. The in uence of phos-phorylation by Akt on the DNA binding activity wasexamined by gel shift ... lost their DNA bindingactivity. These results indicated that the phosphorylation of Thr102, Thr112, and Ser114 inhibited DNA binding activity of dHAND. In this study, we showed that Akt could bind ... This resultindicates that the major phosphorylation sites reside in the region of amino acids 103–132 of dHAND. The region of amino acids 103–132 contains severalserine and threonine residues...

Tài liệu Báo cáo khoa học: Tryptophan tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalis Cofactor assembly and catalytic properties of recombinant enzyme expressed in Paracoccus denitriﬁcans pptx

... substrate carbinolamine intermediate;III, iminoquinone intermediate; IV, product Schiff base intermediate;V, aminoquinol intermediate. In the oxidative half-reaction the ami-noquinol intermediate ... substrates. The lack of inhibition observed with benzylamine (Fig. 5A) in comparison to the inhibition observed with tryptamine(Fig. 5B) suggests differences in binding of the twosubstrates within the ... kinetically in uentialionizations were identiﬁed in plots of klim⁄ Kd of pKavalues 7.1 and 9.3,again with the maximum value realized in the alkaline region. The poten-tial origin of these kinetically...

Tài liệu Báo cáo khoa học: "Enhanced word decomposition by calibrating the decision threshold of probabilistic models and using a model ensemble" pdf

... Another way of classifying ap-proaches is based on the learning aspect during the construction of the morphological model. If the data for training the model has the same struc-ture as the ... cross-validation on datasets ranging from 500 to 2500words with the goal of measuring how the learningimproves with increasing experience in terms of training set size. We want to remind the reader thatour ... Nevertheless, across the entirePR curve none of the algorithms dominates. Onecurve would dominate another if all data points of the dominated curve were beneath or equalto the dominating one....

Tài liệu Báo cáo khoa học: "Identifying Agreement and Disagreement in Conversational Speech: Use of Bayesian Networks to Model Pragmatic Dependencies" docx

... of overlap with any other speakerseconds of silence during the spurtspeech rate in the spurtLexical features:number of words in the spurtnumber of content words in the spurtperplexity of ... testing and the rest of the data fortraining. Table 7 summarizes the performance of our classiﬁer with the different feature sets in thisclassiﬁcation task, distinguishing the case where the four ... “backward-looking”, in the sense that they result from an anal-ysis of context preceding B. For many of them, webuilt equivalent “forward-looking” features that per-tain to the closest utterance of the...

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