Báo cáo khoa học: Origins of DNA replication in the three domains of life pdf
Báo cáo khoa học: Origins of DNA replication in the three domains of life pdf
... and 1L8Q (DnaA). The AAA + domains of both proteins are shown in cyan with ADP indicated in red. The helix-turn-helix (HTH)-containing DNA binding domain of DnaA is in green and the winged helix ... seen in Fig. 4, both proteins possess N-terminal AAA + domains and C-terminal DNA binding domains (DBDs). In DnaA, the DBD contains a helix-turn-helix; in the a...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Nanoparticles can induce changes in the intracellular metabolism of lipids without compromising cellular viability docx
... HIF-1a is involved directly in the accumulation of lipids trig- gered by QDs under the conditions of uncompromised cell survival in the presence of serum. On the basis of the results obtained in this ... storage in LDs. The PI3K ⁄ Akt signaling pathway stimulates lipogenesis via SREBP-1. FAS is the enzyme responsible for the synthesis of palmitate, the prec...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: C fi G base mutations in the CArG box of c-fos serum response element alter its bending flexibility Consequences for core-SRF recognition potx
... provide information on the local repartition of the angles involved in the SRE fos helix bending. Relative effect of bending strain There are several indications of a redistribution of the strains ... Indeed, the change in the array of the hydrogen bonds at thymine of SRE GGfos is probably a sign of perturbation in the hydration scheme along the minor gro...
Ngày tải lên: 07/03/2014, 09:20
Báo cáo khoa học: Key substrate recognition residues in the active site of a plant cytochrome P450, CYP73A1 ppt
... Mutation of all arginines led to a significant loss in protein stability suggesting that they are involved in protein fold structure rather than binding of the substrate. The overall picture of the CYP73A1 ... affect the binding of cinna- mate and the coupling of the hydroxylation reaction [9]. This effect was probably due to a direct interaction of its side-chain w...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo khoa học: A novel phosphorylated glycoprotein in the shell matrix of the oyster Crassostrea nippona pptx
... 1866–1871 of the 1.9 kb cDNA. The relevant Fig. 4. Nucleotide sequence of the 1.9 kb cDNA and deduced amino acid sequence. Numbers on the left indicate the nucleotide positions in the 1.9 kb cDNA sequence (upper) ... green staining. Arrows on the right side of the lanes indicate the position of the 52 kDa component. A weakly stained band in lane A does not corr...
Ngày tải lên: 30/03/2014, 04:20
Tài liệu Báo cáo khoa học: Ionic strength and magnesium affect the specificity of Escherichia coli and human 8-oxoguanine-DNA glycosylases pdf
... address the in uence of the reaction conditions on the binding of Fpg and OGG1 to damaged DNA, we determined K d values for binding under the same conditions as used for the kinetic experi- ments. In ... incorporation of 8-oxoG into DNA during replication [7–9]. Inacti- vation of the GO system increases the mutagenesis rate in bacteria [11] and increases the...
Ngày tải lên: 18/02/2014, 18:20
Báo cáo khoa học: Akt-dependent phosphorylation negatively regulates the transcriptional activity of dHAND by inhibiting the DNA binding activity pdf
... dHAND, we next examine whether the DNA binding activity of dHAND could be affected by Akt phosphorylation. The in uence of phos- phorylation by Akt on the DNA binding activity was examined by gel shift ... lost their DNA binding activity. These results indicated that the phosphorylation of Thr102, Thr112, and Ser114 inhibited DNA binding activity of dHAND. In this s...
Ngày tải lên: 07/03/2014, 16:20
Tài liệu Báo cáo khoa học: Tryptophan tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalis Cofactor assembly and catalytic properties of recombinant enzyme expressed in Paracoccus denitrificans pptx
... substrate carbinolamine intermediate; III, iminoquinone intermediate; IV, product Schiff base intermediate; V, aminoquinol intermediate. In the oxidative half-reaction the ami- noquinol intermediate ... substrates. The lack of inhibition observed with benzylamine (Fig. 5A) in comparison to the inhibition observed with tryptamine (Fig. 5B) suggests differences in binding of...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: "Enhanced word decomposition by calibrating the decision threshold of probabilistic models and using a model ensemble" pdf
... Another way of classifying ap- proaches is based on the learning aspect during the construction of the morphological model. If the data for training the model has the same struc- ture as the ... cross- validation on datasets ranging from 500 to 2500 words with the goal of measuring how the learning improves with increasing experience in terms of training set size....
Ngày tải lên: 20/02/2014, 04:20
Tài liệu Báo cáo khoa học: "Identifying Agreement and Disagreement in Conversational Speech: Use of Bayesian Networks to Model Pragmatic Dependencies" docx
... of overlap with any other speaker seconds of silence during the spurt speech rate in the spurt Lexical features: number of words in the spurt number of content words in the spurt perplexity of ... testing and the rest of the data for training. Table 7 summarizes the performance of our classifier with the different feature sets in this classification task, d...
Ngày tải lên: 20/02/2014, 16:20