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Báo cáo khoa học: Regulation of arginase II by interferon regulatory factor 3 and the involvement of polyamines in the antiviral response potx

Báo cáo khoa học: Regulation of arginase II by interferon regulatory factor 3 and the involvement of polyamines in the antiviral response potx

Báo cáo khoa học: Regulation of arginase II by interferon regulatory factor 3 and the involvement of polyamines in the antiviral response potx

... Biochem 31 8, 212–220.N. Grandvaux et al. IRF -3- mediated antiviral response involves spermineFEBS Journal 272 (2005) 31 20 31 31 ª 2005 FEBS 31 31 Regulation of arginase II by interferon regulatory factor ... IRF -3 responsive, including the gene encoding arginase II (ArgII).ArgII is the extrahepatic isoform of the arginase type enzymes, and ArgI is the hepatic-specific counter-part [16]. The two isoforms ... ArgII expression was inhibited by MG- 132 [20], suggesting indirectly an involvement of NF-jBinArgII regulation. Our study is thus the first directdemonstration of the involvement of IRF -3 in...
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Tài liệu Báo cáo khoa học: Efficient RNA ligation by reverse-joined hairpin ribozymes and engineering of twin ribozymes consisting of conventional and reverse-joined hairpin ribozyme units ppt

Tài liệu Báo cáo khoa học: Efficient RNA ligation by reverse-joined hairpin ribozymes and engineering of twin ribozymes consisting of conventional and reverse-joined hairpin ribozyme units ppt

... stacking of the two domains in the relevantvariant of the hinged conventional hairpin ribozymeleading to enrichment of an extended inactive confor-mation [34 ,35 ]. Because of the single-stranded ... that the sequence and length of the helix between the two loops contain-ing the cleavage ⁄ ligation site, as well the size of the bulge, in uence the exchange efficiency [16 ,32 ,33 ].Therefore, the ... ribozymedomains to that seen in the crystal structure of the con-ventional hairpin ribozyme [21 ,31 ]. Variation in linkerlength and ⁄ or the length of the duplexes flanking the single-stranded linker...
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Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc

Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc

... Ser112, Ser 136 and Ser155 [86]. The dissociation of Bax and Bad from 14 -3- 3 is pro-moted by JNK via phosphorylation of 14 -3- 3 atSer184; this reduces the affinity of 14 -3- 3 for Bax and Bad and translocation ... and increases the release of cytochrome c from mitochon-dria into the cytosol through inactivation with Bcl-xL and Bcl-2 [69]. Inactivated Bax and Bad bind 14 -3- 3, the phosphoserine ⁄ threonine binding ... caspase -3 by binding to caspase -3 in nucleus of human hepatoma Bel-7402 cells duringanti-Fas antibody- and actinomycin D-induced apop-tosis. Biomed Pharmacother 63, 34 3 35 0.Phosphorylation and caspases...
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Tài liệu Báo cáo khoa học: Regulation of the members of the mammalian heat shock factor family doc

Tài liệu Báo cáo khoa học: Regulation of the members of the mammalian heat shock factor family doc

... [102]. In the olfactoryepithelium of 3 6-week-old mice, the expression profile of HSF1 remains constant. However, a significantincrease in the DNA-binding activity of HSF1 can bedetected during the ... transition of HSF1 in the olfactory epithelium warrants furtherinvestigations. In accordance with the fundamentalrole of HSF1 in the heat shock response, the require-ment of HSF1 and HSF4 in development ... thereby facilitating refolding of pep-tides and preventing protein aggregation [32 ]. The dis-covery of an interaction between HSF1 and HSPs,such as Hsp70 ⁄ Hsp40 and HSP90, led to the hypothe-sis...
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Tài liệu Báo cáo khoa học: Regulation of cathepsin B activity by 2A2 monoclonal antibody docx

Tài liệu Báo cáo khoa học: Regulation of cathepsin B activity by 2A2 monoclonal antibody docx

... locatedbetween amino acids 133138 at the exposed part of the cathepsin B molecule near the occluding loop(Fig. 2C). The location of the epitope indicates that the binding of 2A2 mAb might change the conforma-tion ... decrease in the level of the cathepsin B ⁄ cystatin C complex, whereas the level of the complex formed between cathepsin B and the antibody or its Fab increased. This suggests that the binding of the ... identify the epitope in the vicinity of the enzyme’s active site to which 2A2mAb binds, to determine the interference with the binding of substrates and other inhibitors and to iden-tify the mechanism...
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Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt

Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt

... between the dCTP(dUTP)-bindingconformer and the dTTP-binding conformer of dCTPdeaminase. We were not able to identify structuralchanges in the main chain of the subunit, or in the interaction of ... prevented binding of the C-terminalresidues over the active site, or the absence of the C-terminal residues caused the movement of the lip(Fig. 1B). In addition, the Cachain between aminoacid ... in the E 138 A:dTTPstructure. The two copies are designated A and B,originating from the A and B chains in the structure.dTTP binds at the site of the protein shown previouslyto bind the nucleotides...
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Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx

Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx

... activators of the p38 and PtdIns 3- kinase pathways including thr ombin,dexamethasone, angiotensin II and prostaglandins stimulate the expression of CTGF/CCN2 efficiently [8, 43 45]. The role of the cytoskeleton ... prompt termination of the pr otein s ynthesis.These data a dd to the growing body of informationsupporting a preponderant role of p38 in the regulation of gene expression at the level of mRNA ... FBSfor 30 min and then fixed, permeabilized and stained for F-actin with rhodamine-conju-gated phalloidin. Th e effects of toxin B and latrunculin B on the actin filaments wasexamined by preincubating...
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Tài liệu Báo cáo khoa học: Regulation of the actin–myosin interaction by titin doc

Tài liệu Báo cáo khoa học: Regulation of the actin–myosin interaction by titin doc

... T800 and T m–Tnbinding to actin induces a shortening of filament length and an increase in myosin sliding velocity, suggesting a strongsynergy between these two actin binding components in their ... properties, and i t interacts withseveral protein partners such as myosin, a ctin, M protein,C protein, MURF-1, calpain 3, myomesin, a-actinin,nebulin, telethonin and obscu rin. The elastic domains ... actin.Can the diminution of HMM contacts with the N-terminal part of actin account f or the d ecrease in actin–HMM ATPase activity and for the increase in thinfilament sliding velocity while the...
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Báo cáo khoa học: Light-harvesting complex II protein CP29 binds to photosystem I of Chlamydomonas reinhardtii under State 2 conditions doc

Báo cáo khoa học: Light-harvesting complex II protein CP29 binds to photosystem I of Chlamydomonas reinhardtii under State 2 conditions doc

... modelled in blue in Fig. 5 according to the X-ray structure of the LHCIIprotein [30 ]. In order to further test the hypothesis thatphospho-CP29 plays a role in the binding of phospho-LHCII to ... Positions of the peptides in the sequence of the mature CP29 are indicated by corresponding amino acid numbers. The sequences of the peptides1–4 were obtained after in- gel digestion of the putative ... the binding of LHCII to the PSII reaction centre core complex [28,29]. We there-fore suggest that the additional density observed in the S2 LHCI–PSI supercomplex in the vicinity of PsaH isindeed...
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Báo cáo khoa học: Regulation of Cyr61/CCN1 gene expression through RhoA GTPase and p38MAPK signaling pathways Role of CREB and AP-1 transcription factors doc

Báo cáo khoa học: Regulation of Cyr61/CCN1 gene expression through RhoA GTPase and p38MAPK signaling pathways Role of CREB and AP-1 transcription factors doc

... p38 and the inhibition of Rho proteins, by the use of C3 toxin, inhibitsthis response selectively [56]. Additionally, the ability of constitutively active forms of the upstream activators of p38, ... unrelated to the JNK pathways and are likely the result of partial inhibition of the p38 pathway by thisinhibitor as reported previously [32 ].Interestingly, one of the ways in which these signalingmolecules ... protein-1-binding sites bound by the Fra-2.JunD complex cooperate for the regulation of murine laminin alpha3A (lama3A) promoter activity by transforming growth factor- beta. J. Biol. Chem. 2 73, ...
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