Báo cáo khoa học: Adenylyl cyclase Rv0386 from Mycobacterium tuberculosis H37Rv uses a novel mode for substrate selection ppt

... of catalytic mechanisms may actually exist. The gene Rv0386 from Mycobacterium tuberculosis codes for an adenylyl cyclase catalytic domain fused to an AAA-ATPase and a helix-turn-helix DNA-binding ... was partic- ularly remarkable, because asparagine can act as both, a hydrogen-bond donor via its amide group and as a hydrogen-bond acceptor via its carbonyl oxygen atom. In...

Ngày tải lên: 07/03/2014, 21:20

... wall virulence factors When Mycobacteria are stained by Gram staining, they cannot be decolorized by acid alcohol and are there- fore classified as acid-fast bacilli. Acid fastness is largely a ... as regulators of membrane traffic. Science 271, 1533–1539. 84 Maiti D, Bhattacharyya A & Basu J (2000) Lipoarabi- nomannan from Mycobacterium tuberculosis promotes macrophage survival b...

Ngày tải lên: 16/02/2014, 15:20

... Class III contains by far the largest number of ACs, including all mammalian and many bacterial cyclases. All class III ACs must di- merize to be active, because the substrate- binding sites are ... ) 20 °C. AC assays AC activity was measured at 37 °C for 10 min in a volume of 100 lL [34]. Standard reactions contained 50 mm Mycobacterium tuberculosis AC Rv2212 A. Abdel Motaal et al....

Ngày tải lên: 30/03/2014, 10:20

... co- occurrence based measures like mutual informa- tion, Euclidean and Jaccard distance, and found that weight harmonic averaging gives relatively better results. Due to space limitation, we are not able ... Re-ranking Based Approach Yabin Zheng Zhiyuan Liu Lixing Xie State Key Laboratory on Intelligent Technology and Systems Tsinghua National Laboratory for Information Science and...

Ngày tải lên: 20/02/2014, 04:20

... Japan {fatia-s, yosikawa, uemura}@is.aist-nara.ac.jp Masatoshi Yoshikawa Nagoya University Nagoya, 464-8601, Japan Shunsuke Uemura Nara Institute of Science and Technology Nara, 630-0101, Japan Abstract The ... Bilingual Terminology Acquisition from Comparable Corpora and Phrasal Translation to Cross-Language Information Retrieval Fatiha Sadat Nara Institute of Science and Technology Nara,...

Ngày tải lên: 20/02/2014, 16:20

... sulphate, succinic acid, NaOH and appropriate amino acids. Tr ansformations with either linear DNA or plasmids were performed using the modified lithium acetate proce- dure as described [17]. Analysis ... cerevisiae An in vitro assay was established using crude preparation s from of BJ2168 (Table 1) and synthetic CCK-33 as substrate (Fig. 1A) . The nature of the protease performing the cle...

Ngày tải lên: 07/03/2014, 16:20

... transcriptional, translational and post-translational levels. Almost all r factors are constitutively expressed at a basal level and the levels are upregulated through intricate regulatory mecha- nisms ... infected macrophages and animal models. For example, strains mutated for certain r factors control- ling virulence and stress-associated regulons fail to show any loss in virulence in...

Ngày tải lên: 15/03/2014, 09:20

... F-type Na + -AT- Pase from Ilyobacter tartaricus. Science 308, 659–662. 7 Murata T, Yamato I, Kakinuma Y, Leslie AG & Walker JE (2005) Structure of the rotor of the V-type Na + - ATPase from ... topography of the interacting helical faces of subunits a and c from the I. tartaricus ATP synthase has been mapped. According to these data, the essential stator arginine (aR226) is locat...

Ngày tải lên: 16/03/2014, 06:20

... incu- bated at 25 °C for up to 16 days, and aliquots were sampled at regular intervals for analysis by MS and SDS ⁄ PAGE. No degradation of wild-type aB-crystallin was observed after a 9-day incubation, ... the solution of R120G aB-crystallin and a- lactalbumin contained a heavy precipitate, whereas the mixture of wild-type aB-crystallin and a- lactalbumin was clear. The sample of r...

Ngày tải lên: 16/03/2014, 18:20

... and fatty acid pathways. Abbreviations AGT1, alanine-glyoxylate aminotransferase 1; AGT2, alanine-glyoxylate aminotransferase 2; AGXT, alanine-glyoxylate aminotransferase gene; Agxt ) ⁄ ) , alanine-glyoxylate ... short chain; Aco1, aconitase 1; Agt ) , alanine-glyoxylate aminotransferase knockout; Aldh2, aldehyde dehydrogenase 2; Car3, carbonic anhydr- ase 3; Cat, catalase; Dao1, D-amino acid...

Ngày tải lên: 23/03/2014, 03:20