Báo cáo khoa học: Interaction of human stefin B in the prefibrillar oligomeric form with membranes Correlation with cellular toxicity doc

... confirm the binding of < /b> the aggregated forms, albeit to a much smaller extent than that observed for some proteins that bind specifically to membranes, such as the small membrane-binding domains involved in ... a correlation among amyloid fibril formation, interaction < /b> with phospholipids, and cellular toxicity, using a model amyloidogenic protein, human < /b> stefi...

Ngày tải lên: 07/03/2014, 17:20

... resulting in the accu- mulation of < /b> both unmodified cholesteryl esters and glycated apoB in the cells. The rate of < /b> removal of < /b> the latter species is slow, with only 20–30% of < /b> the glycated protein being ... the turnover of < /b> preaccu- mulated protein to be studied in the absence of < /b> further cellular uptake. In these studies, cell dea...

Ngày tải lên: 19/02/2014, 02:20

... of < /b> the interaction < /b> of < /b> viral fusion proteins with membranes and the mechan- ism by which these proteins accelerate the formation of < /b> membrane fusion intermediates are poorly under- stood [2]. In ... that determine peptide–membrane interaction,< /b> and com- bined analysis of < /b> the data obtained provides insights into HGV ⁄ GBV-C–membrane interac...

Ngày tải lên: 07/03/2014, 17:20

... either 0 ° or 90 ° (with respect to the plane of < /b> reflections). In the case of < /b> lipid-bound protein, the spectrum of < /b> the protein was obtained by subtracting the contribution of < /b> the lipid alone, with ... lyso-PtdIns for 20 min before adding the LUVs or preincubating LUVs and then adding ostreolysin. Fluorescence measurements Steady-state intrinsic flu...

Ngày tải lên: 23/03/2014, 21:20

... diseases [47]. Most analyses of < /b> the regulation of < /b> AP-2 and the interactions of < /b> the transcription factor with binding partners, as well as of < /b> the regulation of < /b> target gene expression, have been performed for AP-2a.Upto now, ... differentiated phenotype. For AP- 2b, expression in murine limbs has also been demonstrated. AP- 2b is expressed in t...

Ngày tải lên: 16/02/2014, 09:20

... to proper U6 binding, either directly or indirectly, by affecting the RNA-binding ability of < /b> the resulting heteroheptameric Lsm complex (see Lsm8DC, Lsm818, Lsm881 and Lsm188). Minimal U6 binding by GFP GFP IP Input Lsm7-Myc U6 ... the latter, indicating that in the absence of < /b> an N-terminal domain distinct localization is lacking. Finally, the Lsm1p Sm domain by itse...

Ngày tải lên: 07/03/2014, 02:20

... nucleophilic attack of < /b> the active-site serine, bS1, on the carbonyl carbon of < /b> the amide or ester bond of < /b> the substrate [3] (residues are labelled to indicate the subunit (a or b) and their position in the subunit). ... acid may bind in the acyl- binding site of < /b> PA. Hydrolysis of < /b> p-NPA may thus proceed via reversed binding of < /b> the...

Ngày tải lên: 08/03/2014, 08:20

... described above. The control activity of < /b> TPO was determined in the presence of < /b> inactivated alkaline phosphatase. Action of < /b> a-mannosidase on the inhibitor The inhibitor purified as described above, ... not shown). The absence of < /b> lipids in the structure of < /b> the peroxidase inhibitor, discernible throughout the purification process, was confirmed...

Ngày tải lên: 30/03/2014, 14:20

... to the presence of < /b> a high molar excess of < /b> BSA remaining in the recovery buffer. Therefore, a fixed aliquot of < /b> the protein was incubated with 1 ng of < /b> the indicated probes. After 30 min incubation at ... form with it the 100-kDa complex [21]. Figure 3B shows the binding of < /b> labelled oligomers to the nuclear-enriched fraction of < /b> prot...

Ngày tải lên: 16/03/2014, 13:20

... by splicing, whereas a dashed line in exon 7 of < /b> fragment C represents a dele- tion of < /b> 39 bp. The thick lines indicate intron 6 of < /b> 75 bp in fragments B and C, 3¢ portion of < /b> 35 bp in intron 5 in ... of < /b> alternative promoters in DNASE1 results in no generation of < /b> protein isoforms. The 5¢-UTR of < /b> eukaryotic mRNA in uences the ini...

Ngày tải lên: 19/02/2014, 06:20