Báo cáo khoa học: Unusual venom phospholipases A2 of two primitive tree vipers Trimeresurus puniceus and Trimeresurus borneensis ppt

... 2005 FEBS 3025 Unusual venom phospholipases A 2 of two primitive tree vipers Trimeresurus puniceus and Trimeresurus borneensis Ying-Ming Wang, Hao-Fan Peng and Inn-Ho Tsai Institute of Biological ... explore the venom diversity of Asian pit vipers, we investigated the structure and function of venom phospholipase A 2 (PLA 2 ) derived from two primit...

Ngày tải lên: 07/03/2014, 17:20

... subtree 1 (T), …, # subtree i (T), …, # subtree n (T) ) where # subtree i (T) is the occurrence number of the i th sub -tree type (subtree i ) in T. Since the number of different sub-trees ... ) ii ii i subtree subtree inN nN nN nN KTT T T subtree T subtree T In In nn φ φ ∈∈ ∈∈ =< > = = =∆ ⋅ ⋅ ∑ ∑∑ ∑ ∑∑ (3) where N 1 and N 2 are the sets of nodes in trees T 1 and...

Ngày tải lên: 20/02/2014, 12:20

... A ˚ and 2.6 A ˚ ) and Glu19 (2.7 A ˚ ), and two water molecules (1.7 A ˚ and 1.9 A ˚ ) (Fig. 4E). In M5, the side chains of Asp76 (1.9 A ˚ ) and Asp27 (2.4 A ˚ ) of a symmetry-related molecule and ... sphere of M3 is completed by three other water molecules at distances of 1.6, 2.0 and 1.8 A ˚ , respectively, and by the carboxylate oxy- gens of Asp31 (2.1 A ˚ ) an...

Ngày tải lên: 18/02/2014, 16:20

... enzymes (iPLA 2 ), and platelet-activating factor acetyl hydro- lases [1,5]. The iPLA 2 family consists of two members in mam- malian cells, designated iPLA 2 -VIA and iPLA2-VIB, of which the former ... were found to be independent of the generation of free arachidonic acid or one of its oxygenated metabolites, and may work through regula- tion of the cellular level of p...

Ngày tải lên: 18/02/2014, 17:20

... 23S.5DG b pre-RNA, and the binding of a minimum of n Mg 2+ ions leads to formation of the active complex, whereas binding of a minimum of m Mn 2+ ions forms the inactive complex. The sizes of the active and ... majority of product comes from the highly active fraction. Hence, plots of the percentage of total RNA that is InDG b yield n and m from Eqns 5 and 6. T C. Ku...

Ngày tải lên: 19/02/2014, 07:20

... (2000) Redundant and segregated functions of granule-associated heparin-binding group II subfamily of secretory phospholipases A 2 in the regula- tion of degranulation and prostaglandin D 2 synthesis ... functional aspects of the group II subfamily (types IIA and V) and type X secre- tory phospholipase A 2 s in regulating arachidonic acid release and prostaglandin generati...

Ngày tải lên: 19/02/2014, 16:20

... repre- sentatives of the GI PLA2 family in the Metazoa. Fig. S2. Pfam HMM Logo. Fig. S3. Alignment of Sycon GI PLA2 and Sus GIB PLA2. Fig. S4. Alignment of metazoan N27 GI PLA2s. This supplementary ... the beginning and end of the cDNA and sequenced. Sequence analysis and homology modeling DNA and amino acid sequences were processed, analyzed and aligned with the vector nt...

Ngày tải lên: 06/03/2014, 11:20

... amino acid sequences of Mb and Ngb and the comparison of A B C D Fig. 2. CD characterization of Ngb and Mb at neutral and acidic pH. (A) Far-UV CD spectra of human holoNgb and apoNgb dissolved ... Ngb and H64Q mutant of Ngb was followed by measuring the decrease in intensity of the Soret band (Ngb, k max of 412 nm; H64Q, k max of 406 nm; Mb, k max of 409 nm) in t...

Ngày tải lên: 07/03/2014, 02:20

... There are two pairs of cysteine residues, Cys142 and Cys193 in PhyH-DII and Cys445 and Cys495 in PhyH-DI. These residues are at the same positions as Cys157, Cys206, Cys450 and Cys498 of PhyS [6], ... a six-blade b-propeller containing five four-stranded sheets and one five-stranded sheet. Expression and purification of PhyH, PhyH-DI and PhyH-DII PhyH, PhyH-DI and PhyH-DI...

Ngày tải lên: 14/02/2014, 15:20

... presence of ADP and Mg 2+ and followed by nondenaturing PAGE, the bands of the enzyme and the reactivase were markedly reduced in density, and two bands of the enzymeÆreactivase complexes (upper and ... Positions of DD and its subun- its (a D , b D and c D ) and the reactivase (DD-R) and its subunits (a R and b R ) are indicated on the right of the gels. In (C)...

Ngày tải lên: 15/02/2014, 01:20