Báo cáo khoa học: Secondary transporters of the 2HCT family contain two homologous domains with inverted membrane topology and trans re-entrant loops docx

... free and bound unlabeled C160–RepA and wild-type RepA, with the protein concentration con- stant. The ratio of the apparent F W94 of bound and free RepA was then used to estimate the fraction of ... on the orientation of the AEDANS absorp- tion and emission transition dipoles with respect to the protein axes. For a case where any of these are parallel to t...

Ngày tải lên: 07/03/2014, 04:20

... metal-binding site at the interface of the two domains, which consists of four side chains: two (His31 and His79) from the N-terminal domain and two (Asp165 and His169) from the C-terminal domain ... ligands in the Mn-reconstituted enzyme and an additional water oxy- gen, which, together with the OD2 of Asp165, the NE2 of His79 and the NE2 of His16...

Ngày tải lên: 14/02/2014, 22:20

... AK, the N-terminal regions of the a-subunits serves as catalytic domains, and the C-terminal regions of the a-subunits and the b-subunits act as regulatory domains [10,11]. The regulatory domains ... according to the procheck program [21] is of good quality, with 95.4% of the res- idues in the most favored regions and 4.6% in allowed regions of the R...

Ngày tải lên: 18/02/2014, 08:20

... the cured Oc cells. The Oc cells were cotransfected with the myc-Cardif (wild-type or mutant C508A) and NS3-4A expression vectors. The production of the myc-Cardif and NS3 in these cells was analyzed ... in Fig. 6C. The black and white arrowheads indicate Cardif and the cleaved Cardif, respectively. (C) TRIF is not cleaved in the O cells. The O and Oc cells wer...

Ngày tải lên: 18/02/2014, 16:20

... the integrity of the bound species during gel electrophoresis, the gel and the running buffer were made of H10Mg buffer and were prewarmed to 44 °C before the samples were loaded. The bound and ... polyacrylamide gel with the 5¢-end labeled substrate and 166 n M rPC ribozyme. The positions of the substrate and the substrate-cleavage product on the gel a...

Ngày tải lên: 18/02/2014, 18:20

... from the refinement to calculate the R free . The final R and R free values of the model are 0.21 and 0.22 for the complete data set up to 1.6 A ˚ . The electron density of the whole main chain of the ... reveals that the most similar regions of these proteinases medi- ate interaction of the two b-barrels, formation of the catalytic machinery and struct...

Ngày tải lên: 19/02/2014, 00:20

... accessibility and determines the shape of the active site entry. (e) The structure of the active site is essentially identical with the active sites of the MshB and LpxC proteins. The conservation of catalytically important ... zinc with the N d atom of His12, one of the O d atoms of Asp15 and the N e atom of His113. His113 protrudes from helix a4...

Ngày tải lên: 19/02/2014, 00:20

... when the temperature is raised. The effect of DCMU on the traces was to increase the amplitude of step J with the concurrent decline of step I, and to retard the rise to F m . With dPQ, the J ... depends on the relative amplitude and rate constant of the J–I phase compared to the amplitudes and rate constants of the two other phases. The above sho...

Ngày tải lên: 19/02/2014, 05:20

... analysis of XXXGXXXG, before (A) and after (B) incubation with Cel74A, and the proposed cleavage pattern (C) with the theoretical m ⁄ z value (z in superscript) of the most important fragments (the ... consist of two b-propeller domains [14]. Such a fold has been recognized in a wide range of proteins, including other GHs (clans E, F and J) [3] and hemo- pexin [...

Ngày tải lên: 19/02/2014, 05:20

... broad range of pHs to be compared. The origin of the optimum pH. The position of the optimum pH depends on the amino acid composition and on the organization of the amino acids within the 3D structure. ... is the pH of the solution and N is the number of ionizable residues. Z unf is the partition function of unfolded state and DG unf is the free e...

Ngày tải lên: 19/02/2014, 12:20