Báo cáo khoa học: Characterization of a prokaryotic haemerythrin from the methanotrophic bacterium Methylococcus capsulatus (Bath) ppt

... 5¢-CTTTAACTTGTTGGGCACTGG CATTG-3¢; NP6, 5¢-TTGATCGATTCTGTCTATGCCC CA-3¢ along with the adaptor primers: AP1 (5¢-GTAATAC GACTCACTATAGGGC-3¢) and AP2 (5¢-ACTATAGGG CACGCGTGGT-3¢). Nested PCR was carried ... obtain the full length sequence: OP5, 5¢-GACTGTAACGGTCATGGYACMAYGT-3¢; OP6, 5¢-GATGAAAATCCTAACCTCTCCCCCGCACAG- 3¢; OP7, 5¢-ACTGCACCTACGGCGGGTCGTTGGTACG TG-3¢; NP4, 5¢-GACACCGTAGGTTGAGCCG...

Ngày tải lên: 19/02/2014, 07:20

... native signal peptide was amplified by PCR using the following primers: 5¢ primer, 5¢-GAGCCCGGATCCACCATGAA GGTCTCAATAATT 3¢;3¢ primer, 5¢-CTGACG GAAT TCTTAAACATTAATGCC 3¢. These primers encoded a Kozak ... W81, as observed with M. brassicae CSPMbraA6 [32]. We observed also that BrC15-Ac was able to displace ASA, suggesting that brominated fatty acid and ASA both associated with W81 in the...

Ngày tải lên: 21/02/2014, 03:20

... Journal compilation ª 2011 FEBS 1853 Characterization of a thiamin diphosphate-dependent phenylpyruvate decarboxylase from Saccharomyces cerevisiae Malea M. Kneen 1 , Razvan Stan 1 , Alejandra Yep 2 , ... alternative pathways are avail- able, it is unlikely to play any significant role in the catabolism of the branched-chain amino acids or of methionine. Identification and mutat...

Ngày tải lên: 06/03/2014, 00:21

... control was purchased from Silenius (Hawthorn, Australia) and poly- clonal goat anti-GST antibodies from Pharmacia Biotech Inc. The goat antihuman IgG specific antibody was obtained from Sigma and the ... domain GST fusion proteins migrated as major bands of  50 kDa. After Factor X or thrombin cleavage and removal of GST the I domains appeared as single major bands of approx...

Ngày tải lên: 20/02/2014, 11:20

... VAO and the bacterial hydroxylases is the mode of binding of the FAD cofactor. In VAO, FAD is covalently bound to a histidine, whereas the bacter- ial counterparts contain a tyrosyl-linked FAD ... covalent attachment of the FAD cofactor. The successful in vitro cofactor incorporation shows that the covalent incorporation is an autocatalytic pro- cess. Covalent flavinyla...

Ngày tải lên: 07/03/2014, 09:20

... Rul- fo per la Genetica Medica, Parma, Italy; and the Fondazione Cassa di Risparmio di Ferrara, Italy. We are grateful to Banca del Sangue of Ferrara for pro- viding fresh blood and Dr Amanda Neville ... essen- tial for a detailed understanding of the signal transduction pathway, and should lead to the develop- ment of new drugs [16]. PKC is an attractive candidate as a therap...

Ngày tải lên: 07/03/2014, 05:20

... Characterization of the interaction between the plasma membrane H + -ATPase of Arabidopsis thaliana and a novel interactor (PPI1) Corrado Viotti, Laura Luoni, Piero Morandini and Maria Ida ... further enhances FC-stimulated H + -ATPase activity [22]. Here we report a characterization of the interaction of PPI1 with the H + -ATPase in PM isolated from control and FC-t...

Ngày tải lên: 19/02/2014, 07:20

... Aiba Y, Nakamura K, Namba T, Hirata M, Mazda O, Katsura Y & Narumiya S (1993) Thromboxane A2 receptor is highly expressed in mouse immature thymocytes and mediates DNA fragmentation and apoptosis. ... Grazia U, Felli MP, Vacca A, Farina AR, Maroder M, Cappabianca L, Meco D, Farina M, Screpanti I, Frati L et al. (1994) Positive and negative regulation of the com- posite octamer motif...

Ngày tải lên: 19/02/2014, 16:20

... 3-OH-c-car- otene and lycopene. The values represent ratios of the C 17 and C 19 dialdehydes in the sum of their peak areas calculated by integrating each peak at its individual k max . Data represent ... citral, the mixture of geranial and its cis-isomer neral, is a major component of the aroma of lemon grass and other lemon-scented plants. Geranial is synthesized from...

Ngày tải lên: 07/03/2014, 03:20

... GAT CATGCACTGAAATTTA (2), GATGAAACCTCTCAA ACTG (3), and CATCATCGCTGGACAATGT (4)], using T. R. Dunkern and A. Hatzelmann Characterization of inhibitors of PDE1C FEBS Journal 274 (2007) 4812–4824 ª 2007 The ... Dunkern and A. Hatzelmann Characterization of inhibitors of phosphodiesterase 1C on a human cellular system Torsten R. Dunkern and Armin Hatzelmann Biochemistry 2 Inflam...

Ngày tải lên: 07/03/2014, 05:20