Báo cáo khoa học: Production and utilization of hydrogen peroxide associated with melanogenesis and tyrosinase-mediated oxidations of DOPA and dopamine docx
Báo cáo khoa học: N-Methylation in polylegionaminic acid is associated with the phase-variable epitope of Legionella pneumophila serogroup 1 lipopolysaccharide pptx
... (A) and Western blot with mAb 2625(B) and m Ab LPS-1 (C) of L. pneumophila LPS from wild-type RC1(lane 1) and mu tant 5215 (lane 2). In A, 0.75 lg LPS per lane wasapplied, and 2 lgLPSinBandC.564 ... performed with thispreparation.The1H NMR chemical shifts and the grouping of thesignals in pairs (one pair at dH3.30 and 3.19, and the other atdH3.03 and 2.95; see below) resembled those of ... containingLPS species with different OPS chain-lengths ( Fig. 4B).A ladder-like pattern was observed with differences of upto 10 sugar residues within each f raction and with overlap-ping about...
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Báo cáo khoa học: Production and utilization of hydrogen peroxide associated with melanogenesis and tyrosinase-mediated oxidations of DOPA and dopamine docx
... Production and utilization of hydrogen peroxide associated with melanogenesis and tyrosinase-mediated oxidations of DOPA and dopamine Maristella Mastore1, Lara Kohler2 and Anthony ... Representative chromatograms of the autoxidation and tyrosinase-mediated oxidations of DOPA, with and without cata-lase. Peak profiles represent levels of DOPA in 5 lL samples of separate reaction mixtures ... in real-time the production of H2O2during theautoxidations and tyrosinase-mediated oxidations of DOPA and dopamine. A pulse voltage (+400 mV) main-tained on a sensitive and selective H2O2sensor...
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Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc
... wasrecovered and mixed with an equal volume of SDS buffer(0.2 m Tris, pH 7.5, 0.3 m NaCl, 25 mm EDTA, 2% SDS) and twice extracted with phenol ⁄ chloroform, once extracted with chloroform, and precipitated ... mm Tris, pH 8.0) in the presence of RNAse A(10 lgÆmg)1extract). After 15 h of binding at 4 °C, beadswere washed twice with 1 mL of IgG BB and once with 1 mL of Tev cleavage buffer (Tev CB: ... modulates the fate of transcripts with which it becomes associated. Materials and methodsMaterialsEnzymes were purchased from Invitrogen (Carlsbad, CA,USA) and Roche (Basel, Switzerland); oligonucleotideswere...
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Báo cáo khoa học: Sensitivity to Hsp90-targeting drugs can arise with mutation to the Hsp90 chaperone, cochaperones and plasma membrane ATP binding cassette transporters of yeast docx
... by mutation to Hsp90 itself (within the Hsp82isoform of yeast Hsp90, the point mutations T101I and A587T); with certain cochaperone defects and through theloss of specific plasma membrane ATP ... a wide range of hydrophobic and Fig. 3. Expression of a HSE-LacZ reporter of HSF activity in cellsexpressing the wild-type (p82a), or T101I and A587T mutant forms of Hsp82. Basal and heat-induced ... photographed after 2 days of growth at 30 °ConYPDAintheabsenceorpresenceoftheindicated concentrations of GA or RD. (B) RD and GA sensitivities of strains bearing deletions of the Pdr5 and Snq2 plasma...
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Báo cáo khoa học: Comparative proteomic analysis identifies proteins associated with the development and progression of colorectal carcinoma pot
... revealedthat over-expression of the three proteins was signifi-cantly associated with the genesis and progression of CRC. The functional implications of the alterations inthe levels of these proteins are ... Enlarged images of RhoGDI, S100A9 and LASP-1 in two-dimensionalgels of normal colorectal mucosa, nmCRC and mCRC. (C) Protein expression of RhoGDI, S100A9 and LASP-1 in normal tissue, nmCRC and mCRC ... intensity score of ‡ 2 with at least 50% of malignant cells showing positivestaining was used to classify tumours with high expression(or over-expression), and < 50% of malignant cells with staining...
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Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
... assay.Stereospecificity and substrate specificityThe stereospecificity of T. reesei TYR2 was studied byfollowing the activities on 15 mml -dopa, dl -dopa and d -dopa and 2.5 mml -dopa, dl -dopa and d-tyrosine, and the ... auto-oxida-tion of diphenols and triphenols, control experiments with- out enzyme were performed with all of the polyphenolicsubstrates. Relative enzymatic activities of T. reesei TYR2on monophenols and ... Buchert1 and Kristiina Kruus11 VTT Technical Research Centre of Finland, Espoo, Finland2 Protein Chemistry Research Group and Core Facility, Institute of Biotechnology, University of Helsinki,...
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Báo cáo khoa học: Production and characterization of a thermostable L-threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus docx
... sequence of the expression clone was con-firmed by sequence analysis of both DNA strands. Production and purification of ADHE. coli BL21(DE3) harbouring pSJS1244 was transformed with pWUR78 and a ... The activity of Pf-TDH was significantly increased by the addition of 2mm CoCl2(relative activity to that of the standardreaction 170%) and not by the addition of 2 mmZnCl2or one of the other ... (http://www.ncbi.nlm.nih.gov/blast/) reveals the highest similarity with (putative) TDHs and zinc-containing alcohol de-hydrogenases from archaea and bacteria. Some of themost significant hits of a BLAST search analysis werea...
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Báo cáo khoa học: Production and characterization of a noncytotoxic deletion variant of the Aspergillus fumigatus allergen Aspf1 displaying reduced IgE binding ppt
... pro-teins and characterized from structural and enzymaticpoints of view. The immunoreactivity of these two pro-teins has also been studied.Results Production, isolation, and spectroscopic and structural ... of the NH2-terminal b-hairpin of ribotoxins, we have studied itsinvolvement in functional and immunoreactive proper-ties of the protein. On the basis of these ideas, theallergen Aspf1 and ... 1DE3 and 1R4Y, respectively). Both structures have been fitted to the coordi-nates of the peptide bond atoms of the catalytic residues of the proteins, His50, Glu96 and His137 in a-sarcin and...
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Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx
... forms of theMAPKs were produced as GST-lofMPK3 and GST-lofMPK6. However, when lofMPK3 and lofMPK6proteins were tested for phosphorylation with OXI1,no phosphorylation of lofMPK3 or lofMPK6 ... GST-lofMPK3 and GST-lofMPK6 weremixed with HIS-OXI1 in kinase buffer and [c-32P]-ATP. (B) Recombi-nant kinase-active GST-MPK3 and GST-MPK6 or recombinantkinase-inactive GST-lofMPK3 and GST-lof ... usedas an internal standard. The results are expressed as fold inductioncompared with the time point 0 of untreated plants. Each measure-ment is the mean and standard deviation of three replicates....
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Tài liệu Báo cáo khoa học: Production of biologically active forms of recombinant hepcidin, the iron-regulatory hormone docx
... inflammation of chronic disease [13] and iron-refractory irondeficiency anemia [14].HAMP and Hepc1 encode precursors of 84 and 83amino acids, respectively, which contain typical 24 and 23 amino ... buffer (pH 5) (Fig. 2, lane 5), and found to beTable 1. Primers used for hepcidin cloning and PCR, and primary structure of the purified peptides and proteins. Forward and reverseoligonucleotides ... 6.2 (instead of pH 7 for m1hepc) and the UnoQ chromatography stepwas run at pH 6.2 rather than pH 7. The production yield of m1hepc was slightly lower than that of huhepc(8% instead of 13%, respectively,...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnchuyên đề điện xoay chiều theo dạngNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinChuong 2 nhận dạng rui roQuản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)chuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtMÔN TRUYỀN THÔNG MARKETING TÍCH HỢP