Báo cáo khoa học: Production and utilization of hydrogen peroxide associated with melanogenesis and tyrosinase-mediated oxidations of DOPA and dopamine docx

... (A) and Western blot with mAb 2625 (B) and m Ab LPS-1 (C) of L. pneumophila LPS from wild-type RC1 (lane 1) and mu tant 5215 (lane 2). In A, 0.75 lg LPS per lane was applied, and 2 lgLPSinBandC. 564 ... performed with this preparation. The 1 H NMR chemical shifts and the grouping of the signals in pairs (one pair at d H 3.30 and 3.19, and the other at d H 3.03 and 2.95...

Ngày tải lên: 08/03/2014, 10:20

... Production and utilization of hydrogen peroxide associated with melanogenesis and tyrosinase-mediated oxidations of DOPA and dopamine Maristella Mastore 1 , Lara Kohler 2 and Anthony ... Representative chromatograms of the autoxidation and tyrosinase-mediated oxidations of DOPA, with and without cata- lase. Peak profiles represent levels of D...

Ngày tải lên: 07/03/2014, 17:20

... was recovered and mixed with an equal volume of SDS buffer (0.2 m Tris, pH 7.5, 0.3 m NaCl, 25 mm EDTA, 2% SDS) and twice extracted with phenol ⁄ chloroform, once extracted with chloroform, and precipitated ... mm Tris, pH 8.0) in the presence of RNAse A (10 lgÆmg )1 extract). After 15 h of binding at 4 °C, beads were washed twice with 1 mL of IgG BB and once with 1...

Ngày tải lên: 16/02/2014, 15:20

... by mutation to Hsp90 itself (within the Hsp82 isoform of yeast Hsp90, the point mutations T101I and A587T); with certain cochaperone defects and through the loss of specific plasma membrane ATP ... a wide range of hydrophobic and Fig. 3. Expression of a HSE-LacZ reporter of HSF activity in cells expressing the wild-type (p82a), or T101I and A587T mutant forms of Hsp82. Ba...

Ngày tải lên: 07/03/2014, 21:20

... revealed that over-expression of the three proteins was signifi- cantly associated with the genesis and progression of CRC. The functional implications of the alterations in the levels of these proteins are ... Enlarged images of RhoGDI, S100A9 and LASP-1 in two-dimensional gels of normal colorectal mucosa, nmCRC and mCRC. (C) Protein expression of RhoGDI, S100A9 and...

Ngày tải lên: 23/03/2014, 03:20

... assay. Stereospecificity and substrate specificity The stereospecificity of T. reesei TYR2 was studied by following the activities on 15 mml -dopa, dl -dopa and d -dopa and 2.5 mml -dopa, dl -dopa and d-tyrosine, and the ... auto-oxida- tion of diphenols and triphenols, control experiments with- out enzyme were performed with all of the polyphenolic substrates. Relative...

Ngày tải lên: 19/02/2014, 06:20

... sequence of the expression clone was con- firmed by sequence analysis of both DNA strands. Production and purification of ADH E. coli BL21(DE3) harbouring pSJS1244 was transformed with pWUR78 and a ... The activity of Pf-TDH was significantly increased by the addition of 2mm CoCl 2 (relative activity to that of the standard reaction 170%) and not by the addition of 2 mm Z...

Ngày tải lên: 16/03/2014, 14:20

... pro- teins and characterized from structural and enzymatic points of view. The immunoreactivity of these two pro- teins has also been studied. Results Production, isolation, and spectroscopic and structural ... of the NH 2 - terminal b-hairpin of ribotoxins, we have studied its involvement in functional and immunoreactive proper- ties of the protein. On the basis of the...

Ngày tải lên: 16/03/2014, 19:20

... forms of the MAPKs were produced as GST-lofMPK3 and GST- lofMPK6. However, when lofMPK3 and lofMPK6 proteins were tested for phosphorylation with OXI1, no phosphorylation of lofMPK3 or lofMPK6 ... GST-lofMPK3 and GST-lofMPK6 were mixed with HIS-OXI1 in kinase buffer and [c- 32 P]-ATP. (B) Recombi- nant kinase-active GST-MPK3 and GST-MPK6 or recombinant kinase-inactive GST-lo...

Ngày tải lên: 14/02/2014, 19:20

... inflammation of chronic disease [13] and iron-refractory iron deficiency anemia [14]. HAMP and Hepc1 encode precursors of 84 and 83 amino acids, respectively, which contain typical 24 and 23 amino ... buffer (pH 5) (Fig. 2, lane 5), and found to be Table 1. Primers used for hepcidin cloning and PCR, and primary structure of the purified peptides and proteins. Forward and...

Ngày tải lên: 18/02/2014, 18:20