Báo cáo khoa học: Glycolysis in Entamoeba histolytica Biochemical characterization of recombinant glycolytic enzymes and flux control analysis ppt

... 2005 FEBS 1783 Glycolysis in Entamoeba histolytica Biochemical characterization of recombinant glycolytic enzymes and flux control analysis Emma Saavedra, Rusely Encalada, Erika Pineda, Ricardo ... The analysis of the kinetic properties and the initial control flux analysis reported here suggest that PPDK, but not PPi-PFK, may indeed exert significant...

Ngày tải lên: 07/03/2014, 17:20

... role of the C-terminal domain in the general folding of the enzyme and, hence, on its activity. This study points to the important role of residues at the IN C-terminal domain in the folding and ... the integration of the DNA copy of the retroviral genome into the host cell DNA. The C-terminal domain of IN is involved in DNA binding and enzyme multimerization. We pr...

Ngày tải lên: 23/03/2014, 15:21

... to unfolding of the protein, a decrease in its overall solu- bility and has detrimental effects on chaperone func- tion by perturbing the putative role of the C-terminal extension in maintaining the integrity ... Micromass). Chaperone activity of R120G aB-crystallin Reduction assay Bovine pancreas insulin (45 lm) was incubated in the pres- ence of increasing amounts of wild-...

Ngày tải lên: 16/03/2014, 18:20

... (b) the use of an extrinsic promoter (P1) and an alternative, intrinsic promoter (P2) in the 5¢-end (leading to the formation of TA and DM classes of isoforms, respectively) [13]; and (c) alternative ... several combinations between differential N-ter- minal domain and C-terminal domain [15]. Despite the rarity of p73 mutations, overexpression of p73 isoforms is common in...

Ngày tải lên: 29/03/2014, 09:20

... aggregation of Ab in vitro [19,28,29]. The binding of Cu 2+ and Zn 2+ to monomeric Ab has been extensively studied and occurs via three histidines (His6, His13, and His14) and a fourth ligand suggested to ... fibrillar and the metal-bound aggregate forms is therefore of interest with respect to under- standing the mode of assembly and pinpointing the mechanism by which me...

Ngày tải lên: 30/03/2014, 01:20

... constraints in the field of corefer- ence resolution evolves from the interplay of intra- sentential binding constraints and the transitivity of the anaphoric relation. Consider the following sentence ... treated intensionally in a Zero-One Inte- ger Linear Programming (ILP) framework which is geared to find the optimal and coherent partition of coreference sets given a number...

Ngày tải lên: 31/03/2014, 20:20

... 1C). Increased nucleolin/C23 protein levels in Hsp70-transfected cells in response to H 2 O 2 -induced apoptosis We next examined the expression of nucleolin ⁄ C23 protein in these cells in response ... Research Program of China (2007CB512007), the National Natural Science Foundation of China (30700290) and Special Funds for PhD Training from The Ministry of Education of C...

Ngày tải lên: 16/02/2014, 09:20

... [8], and the cleavage of DNA by alkali [31]. The method allows easy detection of DNA–protein interactions via the N7 position of guanines in the major groove and via the N3 position of adenines in the ... pattern and a more open chromatin structure over the MMTV chromatin, and with specific acetylation and remodeling events in the promoter region. Specifically, a reposit...

Ngày tải lên: 19/02/2014, 12:20

... binding of oxygen and other strong-field ligands. The heme domain permits kinase activity in the absence of bound ligand, but when the appropriate exogenous ligand is bound, this domain turns off ... PAS containing proteins were involved in the sensing of oxygen, redox or light. PAS domains were first found in eukaryotes, and were named after homology to the Drosophila period pr...

Ngày tải lên: 19/02/2014, 12:20

... diphtheria toxin, ricin, or ricin A chain. CHO cells were incubated for 4 h with varying concentrations of DT (A), ricin (B), or ricin A chain (C). In (D), LEX2 cells and their parental cells were incubated for ... between saporin and ricin A chain sensitization were unlikely to reflect possible differences in the intrinsic toxin activities. Furthermore, our evaluations of toxin se...

Ngày tải lên: 07/03/2014, 05:20