Báo cáo khoa học: Glycolysis in Entamoeba histolytica Biochemical characterization of recombinant glycolytic enzymes and flux control analysis ppt
Báo cáo khoa học: Glycolysis in Entamoeba histolytica Biochemical characterization of recombinant glycolytic enzymes and flux control analysis ppt
... 2005 FEBS 1783 Glycolysis in Entamoeba histolytica Biochemical characterization of recombinant glycolytic enzymes and flux control analysis Emma Saavedra, Rusely Encalada, Erika Pineda, Ricardo ... The analysis of the kineticproperties and the initial control flux analysis reportedhere suggest that PPDK, but not PPi-PFK, mayindeed exert significant control of glycolytic flux in E. histolytica. ... objective of understanding howthe glycolytic flux in E. histolytica is controlled, wecloned the genes, and overexpressed, purified and determined the kinetic parameters of the 10 glycolytic enzymes...
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Báo cáo khoa học: Mutations in the C-terminal domain of ALSV (Avian Leukemia and Sarcoma Viruses) integrase alter the concerted DNA integration process in vitro pot
... role of the C-terminal domain in the general folding of the enzyme and, hence, on its activity. This study points to the importantrole of residues at the IN C-terminal domain in the folding and ... theintegration of the DNA copy of the retroviral genome intothe host cell DNA. The C-terminal domain of IN is involved in DNA binding and enzyme multimerization. We previ-ously performed single ... activities of IN [25,26], and is essential for the formation of IN oligomers [30–38]. In this study, we analysed several pointsmutants in the C-terminal domain of ALSV IN and examined their...
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Báo cáo khoa học: R120G aB-crystallin promotes the unfolding of reduced a-lactalbumin and is inherently unstable ppt
... tounfolding of the protein, a decrease in its overall solu-bility and has detrimental effects on chaperone func-tion by perturbing the putative role of the C-terminalextension in maintaining the integrity ... Micromass).Chaperone activity of R120G aB-crystallinReduction assayBovine pancreas insulin (45 lm) was incubated in the pres-ence of increasing amounts of wild-type or R120G aB-crys-tallin in 50 m m phosphate ... combine to diminish their chaperone ability[22,24,28]. The positive charge of R116 in aA-crystallinhas been implicated as being critical in maintainingstructural integrity [28]. In this investigation,...
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Báo cáo khoa học: Sp1 binds to the external promoter of the p73 gene and induces the expression of TAp73c in lung cancer doc
... (b) the use of anextrinsic promoter (P1) and an alternative, intrinsicpromoter (P2) in the 5¢-end (leading to the formation of TA and DM classes of isoforms, respectively) [13]; and (c) alternative ... several combinations between differential N-ter-minal domain and C-terminal domain [15].Despite the rarity of p73 mutations, overexpression of p73 isoforms is common in several types of cancer[14,16], ... opposing roles, mainly the apoptotic TAisoform(s) and the antiapoptotic DM isoforms. In gen-eral, TAp73 isoforms regulate the transcription of DMp73 isoforms, which, in turn, act as dominant...
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Báo cáo khoa học: Quenched hydrogen ⁄deuterium exchange NMR characterization of amyloid-b peptide aggregates formed in the presence of Cu2+ or Zn2+ ppt
... aggregation of Ab in vitro [19,28,29]. Thebinding of Cu2+ and Zn2+to monomeric Ab has beenextensively studied and occurs via three histidines(His6, His13, and His14) and a fourth ligand suggestedto ... fibrillar and the metal-bound aggregateforms is therefore of interest with respect to under-standing the mode of assembly and pinpointing themechanism by which metals guide the path of aggre-gation ... containing 50 lM Ab(1–40) (solid line) and Ab(1–42) (broken lines) were started immediately after the addition of Cu2+[(iii) and (iv)], Zn2+[(i) and (ii)] or EDTA [(v) and (vi)] and continued...
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Báo cáo khoa học: "Optimization in Coreference Resolution Is Not Needed: A Nearly-Optimal Algorithm with Intensional Constraints" ppt
... constraints in the field of corefer-ence resolution evolves from the interplay of intra-sentential binding constraints and the transitivity of the anaphoric relation. Consider the followingsentence ... treated intensionally in a Zero-One Inte-ger Linear Programming (ILP) framework which isgeared to find the optimal and coherent partition of coreference sets given a number of candidate pairs and ... Zero-One Integer LinearProgramming (ILP)The algorithm in (Balas, 1965) solves Zero-One Integer Linear Programming (ILP), where aweighted linear function (the objective function) of binary variables...
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Tài liệu Báo cáo khoa học: Nucleolin/C23 mediates the antiapoptotic effect of heat shock protein 70 during oxidative stress pptx
... 1C).Increased nucleolin/C23 protein levels in Hsp70-transfected cells in response toH2O2-induced apoptosisWe next examined the expression of nucleolin ⁄ C23protein in these cells in response ... Research Program of China (2007CB512007),the National Natural Science Foundation of China(30700290) and Special Funds for PhD Training fromThe Ministry of Education of China (20060533009).References1 ... oxidativestress induces nucleolin ⁄ C23 cleavage and apoptosis[14], and that nucleolin downregulation induces apop-tosis in C2C12 cells [15]. These data suggest the roles of nucleolin ⁄ C23 in oxidative...
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Tài liệu Báo cáo khóa học: Trichostatin A reduces hormone-induced transcription of the MMTV promoter and has pleiotropic effects on its chromatin structure pptx
... [8], and the cleavage of DNA by alkali [31]. The method allows easy detection of DNA–protein interactions via the N7 position of guanines in the major groove and via the N3 position of adenines in the ... pattern and a more open chromatin structureover the MMTV chromatin, and with specific acetylation and remodeling events in the promoter region. Specifically, arepositioning of initially randomly ... containingminichromosomesTreatment of oocytes with deacetylase inhibitors such asTSA may change the bulk acetylation pattern of histones, and in this way alter the structure of chromatin incorpor-ating...
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Tài liệu Báo cáo khóa học: The PAS fold A redefinition of the PAS domain based upon structural prediction ppt
... binding of oxygen and other strong-fieldligands. The heme domain permits kinase activity in theabsence of bound ligand, but when the appropriateexogenous ligand is bound, this domain turns off ... PAS containing proteins were involved in thesensing of oxygen, redox or light. PAS domains were firstfound in eukaryotes, and were named after homology tothe Drosophila period protein (PER), ... sensory domains,PAS domains are located in the cytoplasm [9] and are found in serine/threonine kinases [3], histidine kinases [10], photo-receptors and chemoreceptors for taxis and tropism [11],cyclic...
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Báo cáo khoa học: Lipopolyamine treatment increases the efficacy of intoxication with saporin and an anticancer saporin conjugate doc
... diphtheria toxin, ricin, orricin A chain. CHO cells were incubated for4 h with varying concentrations of DT (A),ricin (B), or ricin A chain (C). In (D), LEX2cells and their parental cells were incubatedfor ... betweensaporin and ricin A chain sensitization were unlikelyto reflect possible differences in the intrinsic toxinactivities. Furthermore, our evaluations of toxin sensi-tization involved internal standard ... saporin versus ricin A chain. Thedistinct pattern of lipopolyamine-induced sensitizationto ricin A chain could result from membrane destabili-zation by ricin A chain [30,31] and or lipopolyaminedisruption...
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