Báo cáo khoa học: Increased NADPH concentration obtained by metabolic engineering of the pentose phosphate pathway in Aspergillus niger docx

... levels of NADPH in Aspergillus niger FEBS Journal 272 (2005) 1313–1325 ª 2005 FEBS 1325 Increased NADPH concentration obtained by metabolic engineering of the pentose phosphate pathway in Aspergillus ... limited by low availab- ility of NADPH. With the purpose of increasing the NADPH concentra- tion and ⁄ or the flux through the pentose...

Ngày tải lên: 07/03/2014, 17:20

... M ¨ uhlh ¨ auser. 2007. Au- tomatically assessing the post quality in online discus- sions on software. In Proceedings of the 45th An- nual Meeting of the ACL on Interactive Poster and Demonstration Sessions, ... examination of the feature weights learned by our machine learning classifiers. Specifically, we shed light on an ongoing debate in the deception literature reg...

Ngày tải lên: 07/03/2014, 22:20

... Values are the mean of six replicates. The specific uptake was determined by subtracting the uptake counts in the absence of inhibitor from the uptake counts in the presence of cocaine or S-cita- lopram, ... To distinguish total binding from non-specific binding, cells were incubated for 10 min in either PBSCM alone or PBSCM containing 200 lm cocaine for inhibition of...

Ngày tải lên: 14/02/2014, 21:20

... 2A). The zinc ions are required for the structural integrity of the protein, as mutation of any of the cysteine residues involved in zinc coordina- tion results in protein unfolding [22]. The ... element-binding (CREB) binding protein (CBP) [23], a peptide from the Win motif of MLL1 bound to the WD40 repeat protein, WDR5 [24,25] and the C-terminal SET domain in...

Ngày tải lên: 16/02/2014, 14:20

... loss in the AD brain. Further investigation of the increased neurotrophic activity of the AD brain revealed that it correlated with the loss of a specific neuroinhibitory factor, rather than the ... examining the role of GIF in AD, linked to its initial discovery as a factor deficient in the AD brain. There remains no clear con- sensus on the role of GIF in...

Ngày tải lên: 16/02/2014, 15:20

... simultaneously interacts with the main phosphate of the substrate [32]. The closer positioning of the N-terminus to the main protein body is essential for the activity and, together with the 87–104 ... 23 diminished the midpoint of the thermal denaturation curve (T m )by7 °Candthefree energy of unfolding (DG U )by1 1kJÆmol )1 . These values are in very close ag...

Ngày tải lên: 19/02/2014, 12:20

... time. The N-terminal end sequencing of one of the chymotryp- sin-digested fragments revealed that the junction region between the C-terminal end of the histidine tag and the N-terminal end of the ... binding motif similar to that of lambdoid phages, indicating that this half of the /11 repressor most likely participates in the binding of operator DNA. An N-te...

Ngày tải lên: 07/03/2014, 00:20

... implicated in retraction of the PilA4-comprising DNA translocator transporting the DNA through the periplasmic space. Binding of the DNA to the DNA-binding protein ComEA on the surface of the inner ... mutations in either pilW or pilQ significantly affect the location of the other protein in the outer membrane. Furthermore, no PilA4 was present in the oute...

Ngày tải lên: 07/03/2014, 12:20

... protein linked to the C-terminal end of a composite protein GFP-GST-PAD in the pEGFP-GST-PAD vector described in Fig. 1B. Dashed lines indicate the same amino acids in the sequence as shown at the ... Trn-1 binding sequence has been found in these naturally occurring proteins [12,17–19]. hnRNP D ⁄ AUF1 consists of two RNA binding domains (RBDs) and has a high content of...

Ngày tải lên: 07/03/2014, 21:20

... Cu 2+ prevents the binding of these mAbs as shown by ELISA; Cu 2+ does not inhibit the binding of these mAbs to rPrP (results not shown). Discussion Aggregation of PrP is an essential step in the conver- sion ... Cu 2+ -induced rPrP aggregation. We suggest that mAb 8B4 is able to align the rPrP in the same orientation, in parallel, pairing the N-terminus of two PrP...

Ngày tải lên: 16/03/2014, 04:20