Báo cáo khoa học: Kinetic and binding studies with purified recombinant proteins ferredoxin reductase, ferredoxin and cytochrome P450 comprising the morpholine mono-oxygenase from Mycobacteriumsp strain HE5 ppt
Báo cáo khoa học: Kinetic and binding studies with purified recombinant proteins ferredoxin reductase, ferredoxin and cytochrome P450 comprising the morpholine mono-oxygenase from Mycobacteriumsp. strain HE5 ppt
... binding studies with purified recombinant proteins ferredoxin reductase, ferredoxin and cytochrome P450 comprising the morpholine mono-oxygenase from Mycobacterium sp. strain HE5 Bernhard Sielaff and ... redox partners from different sources [5,9,17–19]. The expression and purification of the ferredoxin reductase FdRmor, the ferredoxin Fdmor and the mono-oxygenase P450 morenabled now the first successful ... specificfor the respective P450 and no specific reductase wasidentified for any of these ferredoxins. The specificreductase of the P450 morsystem has been recentlyidentified and the recombinant...
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Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf
... 0.01 lm, and that of the D70G mutant was 0.196 ± 0.02 lm.Effects of ligands on steady-state kinetics The effects of the ligands (L: tyramine, serotonin and ben-zalkonium) on the AAA and esterase ... of the wild-type enzyme:7.3-fold in the presence of o-NPA, 5.2-fold with ASCh,three-fold with ATMA and unaffected with o-NAC. The actual binding site of serotonin cannot be inferred from these ... ofbenzalkonium for the enzyme–substrate complex. Thisresult is consistent with the proposal that the binding site for benzalkonium is either at PAS or in the activesite gorge close to PAS. The binding...
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Tài liệu Báo cáo khoa học: Secondary substrate binding strongly affects activity and binding affinity of Bacillus subtilis and Aspergillus niger GH11 xylanases docx
... minor)role in the hydrolysis of oligomeric substrates. The SBS is located too far from the active site to influence the binding and catalysis of these substrates in the active site. The same statement ... that theseSBS residues are important for the activity on starch and that they play a role in the binding of the enzymeto bacteria of the oral cavity [15]. Nielsen et al. [9]concluded that the ... water-unextractable AX, the reaction with water-unextractableAX was terminated by first separating the nonsolubilized from the solubilized material by filtration and by thenimmediately mixing the filtrate with 230...
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Báo cáo khoa học: Transmembrane helix 12 plays a pivotal role in coupling energy provision and drug binding in ABCB1 pot
... during the 300 min incubation, and the extents of labellingwere quantified in comparison with that found with cysteine-less ABCB1 and the G324C isoform. The G324C mutant was assigned as the positive ... with the hydrophobic (CM) and zwitterionic (BM) probes,on the basis of the extent and rapid half-life of the interactions. In contrast, the hydrophilic FM displayedonly partial labelling, with ... studying the topography ofTM12 in the homology model. First, the midregion of the helix, i.e. V982–G984, was rigid with respect to the intracellular and extracellular sections of the helix inthe...
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Báo cáo khoa học: Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation potx
... the early S phase with adouble thymidine block. We released cells from the block and fixed them at the time points indicated inFig. 5C, and then analyzed the DNA contents of the cells by flow ... cells(Fig. S1). On the other hand, the northern blotting and quantitative real-time PCR analysis also showed that the expression of differentiation marker genes such asfgf5, gata4 and gata6 was not ... after the release, the DNA content patterns were essentially the same inPtb) ⁄ ) and wild-type ES cells, and cells were at the end of the S phase in this period (Fig. 5C, shaded ingray). These...
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Báo cáo khoa học: Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl–tRNA synthetase and determination of its oligomeric structure in solution pptx
... werestable to further proteolytic attack. From the N-termi-nal sequence and the mass of the fragments, and from the kinetics of the process (Figs S7–S9 and Table S5)we concluded that the main sites ... from the atomic coordinates of the discriminating Tt-GluRS (PDBfiles: 1n75 for the complex with ATP; 1j09 for the complex with ATP and Glu; 1n77 for the complex with tRNA and ATP; 1n78 for the ... complex with tRNA and GoA; 1g59 for the complex with tRNA) and of the nondiscriminatingTe-GluRS (PDB file 2cfo for the complex with Glu) and were used to analyse the oligomeric composition of all the samples....
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Báo cáo khoa học: Kinetic characterization of methionine c-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis ppt
... concentration of 2 mm) and the kinetic parameters of recombinant EhMGL1 and EhMGL2 (Tables 1 and 2). Despite the heterogeneityof the EhMGL1 preparation used in the previousstudy [13], the kinetic constants ... of the BamHI site in the vector and the XbaI site in the EhMGL1 gene, the product of the nes-ted PCR was replaced with the corresponding region inpGEX–EhMGL1 [13] to produce pGEX–EhMGL1fl. The following ... investigated whether the recombinant EhMGL wasmodified after the incubation with TFM by examining the mobility of the proteins by SDS ⁄ PAGE. When recombinant EhMGL1 or EhMGL2 was incubated with TFM,...
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Báo cáo khoa học: "Effect of intern’s consecutive work hours on safety, medical education and professionalism"
... Indeed, the results of these studies do suggest that the ACGME did notgo far enough. But how far is enough and at what point do the increased errors associated with more frequent hand-offsoffset the ... http://ccforum.com/content/9/5/528We agree with the recommendation that further researchshould study the effects of sleep deprivation and workschedule interventions on the performance of upper-levelresidents and other medical ... errors.”Differences in the incidence of serious errors were analyzedusing rates (per patient-day), and therefore the fact that therewere more patient-days in the traditional schedule cannotexplain the results....
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Tài liệu Báo cáo khoa học: Diol dehydratase-reactivating factor is a reactivase – evidence for multiple turnovers and subunit swapping with diol dehydratase pdf
... incubated with the reactivase in the presence of ADP and Mg2+ and followed by nondenaturing PAGE, the bands of the enzyme and the reactivase were markedly reducedin density, and two bands of the enzymeÆreactivasecomplexes ... analyses. Both band i and band vi of the enzymeÆreactivase complexes comprised all of the subunits from the enzyme (a, b, and c) and the reacti-vase (a and b) (Fig. 5B,C, lanes i and vi). Subunitcompositions ... 1 and 1 : 1,respectively. These results indicated that the upper and lower bands of the reactivase correspond to the aRÆaRbR and (aRbR)2complexes, respectively. The thin band onthe...
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Tài liệu Báo cáo khoa học: Epidermal growth factor receptor in relation to tumor development: EGFR gene and cancer ppt
... and 28%, respectively.Structure of the ERBB proteins and diversity of their ligands The EGFR gene is located on chromosome 7p12-13 and codes for a 170kDa receptor tyrosine kinase. AllERBB proteins ... and ERBB4.NRG1 and NRG2 bind to both ERBB3 and ERBB4,whereas NRG3 and NRG4 only bind to ERBB4 [3].Although these ligands show redundancy, heparin- binding- EGF is the only ligand whose absence ... such as gefitinib and erlotinib. Here,we review the discovery of EGFR, the EGFR signal transduction pathway and mutations of the EGFR gene in lung cancers and glioblastomas. The biological significance...
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