Báo cáo khoa học: Kinetic and binding studies with purified recombinant proteins ferredoxin reductase, ferredoxin and cytochrome P450 comprising the morpholine mono-oxygenase from Mycobacteriumsp strain HE5 ppt
Báo cáo khoa học: Kinetic and binding studies with purified recombinant proteins ferredoxin reductase, ferredoxin and cytochrome P450 comprising the morpholine mono-oxygenase from Mycobacteriumsp. strain HE5 ppt
... binding studies with purified recombinant proteins ferredoxin reductase, ferredoxin and cytochrome P450 comprising the morpholine mono-oxygenase from Mycobacterium sp. strain HE5 Bernhard Sielaff and ... redox partners from different sources [5,9,17–19]. The expression and purification of the ferredoxin reductase FdR mor , the ferredoxin Fd mo...
Ngày tải lên: 07/03/2014, 16:20
Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf
... 0.01 lm, and that of the D70G mutant was 0.196 ± 0.02 lm. Effects of ligands on steady-state kinetics The effects of the ligands (L: tyramine, serotonin and ben- zalkonium) on the AAA and esterase ... of the wild-type enzyme: 7.3-fold in the presence of o-NPA, 5.2-fold with ASCh, three-fold with ATMA and unaffected with o-NAC. The actual binding site of serotonin...
Ngày tải lên: 18/02/2014, 17:20
Tài liệu Báo cáo khoa học: Secondary substrate binding strongly affects activity and binding affinity of Bacillus subtilis and Aspergillus niger GH11 xylanases docx
... minor) role in the hydrolysis of oligomeric substrates. The SBS is located too far from the active site to influence the binding and catalysis of these substrates in the active site. The same statement ... that these SBS residues are important for the activity on starch and that they play a role in the binding of the enzyme to bacteria of the oral cavity [15]. Nie...
Ngày tải lên: 14/02/2014, 19:20
Báo cáo khoa học: Transmembrane helix 12 plays a pivotal role in coupling energy provision and drug binding in ABCB1 pot
... during the 300 min incubation, and the extents of labelling were quantified in comparison with that found with cysteine-less ABCB1 and the G324C isoform. The G324C mutant was assigned as the positive ... with the hydrophobic (CM) and zwitterionic (BM) probes, on the basis of the extent and rapid half-life of the interactions. In contrast, the hydrophilic FM di...
Ngày tải lên: 06/03/2014, 22:21
Báo cáo khoa học: Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation potx
... the early S phase with a double thymidine block. We released cells from the block and fixed them at the time points indicated in Fig. 5C, and then analyzed the DNA contents of the cells by flow ... cells (Fig. S1). On the other hand, the northern blotting and quantitative real-time PCR analysis also showed that the expression of differentiation marker genes such as fgf...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl–tRNA synthetase and determination of its oligomeric structure in solution pptx
... were stable to further proteolytic attack. From the N-termi- nal sequence and the mass of the fragments, and from the kinetics of the process (Figs S7–S9 and Table S5) we concluded that the main sites ... from the atomic coordinates of the discriminating Tt-GluRS (PDB files: 1n75 for the complex with ATP; 1j09 for the complex with ATP and Glu; 1n77 for th...
Ngày tải lên: 07/03/2014, 03:20
Báo cáo khoa học: Kinetic characterization of methionine c-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis ppt
... concentration of 2 mm) and the kinetic parameters of recombinant EhMGL1 and EhMGL2 (Tables 1 and 2). Despite the heterogeneity of the EhMGL1 preparation used in the previous study [13], the kinetic constants ... of the BamHI site in the vector and the XbaI site in the EhMGL1 gene, the product of the nes- ted PCR was replaced with the corresponding regio...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: "Effect of intern’s consecutive work hours on safety, medical education and professionalism"
... Indeed, the results of these studies do suggest that the ACGME did not go far enough. But how far is enough and at what point do the increased errors associated with more frequent hand-offs offset the ... http://ccforum.com/content/9/5/528 We agree with the recommendation that further research should study the effects of sleep deprivation and work schedule interventions...
Ngày tải lên: 25/10/2012, 10:45
Tài liệu Báo cáo khoa học: Diol dehydratase-reactivating factor is a reactivase – evidence for multiple turnovers and subunit swapping with diol dehydratase pdf
... incubated with the reactivase in the presence of ADP and Mg 2+ and followed by nondenaturing PAGE, the bands of the enzyme and the reactivase were markedly reduced in density, and two bands of the enzymeÆreactivase complexes ... analyses. Both band i and band vi of the enzymeÆreactivase complexes comprised all of the subunits from the enzyme (a, b, and c) an...
Ngày tải lên: 15/02/2014, 01:20
Tài liệu Báo cáo khoa học: Epidermal growth factor receptor in relation to tumor development: EGFR gene and cancer ppt
... and 28%, respectively. Structure of the ERBB proteins and diversity of their ligands The EGFR gene is located on chromosome 7p12-13 and codes for a 170kDa receptor tyrosine kinase. All ERBB proteins ... and ERBB4. NRG1 and NRG2 bind to both ERBB3 and ERBB4, whereas NRG3 and NRG4 only bind to ERBB4 [3]. Although these ligands show redundancy, heparin- binding- EGF is th...
Ngày tải lên: 16/02/2014, 09:20