Báo cáo khoa học: Characterization of the tRNA and ribosome-dependent pppGpp-synthesis by recombinant stringent factor from Escherichia coli pot

... 685–695 ª 2005 FEBS 695 Characterization of the tRNA and ribosome-dependent pppGpp-synthesis by recombinant stringent factor from Escherichia coli Rose-Marie Knutsson Jenvert 1,2 and Lovisa Holmberg ... the P-sites of the 30S and 50S subunits and the tRNA Phe bound in the A-site of the 30S and 50S sub- unit [28]. Moreover, a tRNA Met f was...

Ngày tải lên: 07/03/2014, 16:20

... analy- sis of the LPS from related species is important in order to gain an understanding of the molecular basis of their biological properties, the host specificity and diversity of members of this ... mannose residues. The b- configuration of the Man F followed from the observa- tion of intraresidual strong NOEs between H-1 and H-3, H-5, and also from th...

Ngày tải lên: 30/03/2014, 10:20

... FEBS ([1] and references therein). The auto-inhibitory action of the C-terminus is suppressed, besides by pH, by lysophospholipids and by 14-3-3 proteins ([1] and ref- erences therein). The latter ... of the first 88 amino acids of PPI1 may participate in the interaction with the H + -ATPase and makes PPI1 588 His 6 a suitable tool to study the mechanism of...

Ngày tải lên: 19/02/2014, 07:20

... SNO2, SNO3, SNZ2 and SNZ3 complement the defect. The relationship of all of these genes remains to be clarified. Expression and purification of Sno1p and Snz1p In light of the similarity in the amino ... hydrolysis mediated by the complex of Sno1p with a His-tag and Snz1p. The reaction was carried out in 1 mL of 50 m M Tris/HCl, pH 8.0, in the presence of 1...

Ngày tải lên: 19/02/2014, 12:20

... transcription factor of the Ets-family is involved in the regulation. The members of the Ets-family of transcription factors bind to specific purine-rich sequences with a core motif of GGAA/T and control the ... CCAAT box at 324 bp upstream of the major transcription start site. The presence of a CCAAT box and the absence of a TATA box seem to be one of t...

Ngày tải lên: 21/02/2014, 03:20

... Cleavage of the C9–C10 double bond (a) results in products 1 and 2 from I, products 1 and 3 from II, and products 3 and 4 from III. The cleavage of (b) leads to product 4 from II, and product 6 from ... that the preference of OsCCD1 for the C5¢–C6¢ and C7¢–C8¢ double bonds depends on the nature of the substrate. The cleavage of a sole double bond...

Ngày tải lên: 07/03/2014, 03:20

... work was supported by grants from the Grant Agency of the Ministry of Education of the Slovak Republic and the Slovak Academy of Sciences 1 ⁄ 3241 ⁄ 06 (to A. Horva ´ th), and the Comenius University ... excluded the possibility that the  2.2 MDa large complex is just an oligomer of the small form. The different influences of DPI on the two forms could...

Ngày tải lên: 07/03/2014, 09:20

... dramatic loss of affinity and potency. To analyze the structural implications of these biological results, the conformational preferences of the SP analogues were analyzed by NMR spectroscopy and minimum-energy ... removal of the last Na-Boc-protecting group, the resin was dried in vacuo. The peptide resin was transferred into the Teflon vessel of an HF apparatus a...

Ngày tải lên: 08/03/2014, 02:21

... forms: 38 and 40 kDa (Fig. 5A). The profile of recombinant GluV8 was essentially identical to that of native GluV8, except for the presence of the non- degraded 41–44 kDa bands of the recombinant ... it was concluded that the full-length form of GluV8 could be recovered quantitatively by the suppression of self-degradation, either by the use of the GluSE...

Ngày tải lên: 16/03/2014, 06:20

... of buffer A. The column was then washed with further 10 column volumes of buf- fer A, and thereafter the bound proteins were eluted by applying a linear gradient of 0–0.6 mm NaCl in a total of 40 ... All these positions are located at the surface of the jelly roll domain of Bga1 and thus most probably are glycosylated in vivo. To further substantiate these findings, i...

Ngày tải lên: 16/03/2014, 11:20