Báo cáo khoa học: A ‘pure’ chemoattractant formylpeptide analogue triggers a specific signalling pathway in human neutrophil chemotaxis potx

... A ‘pure’ chemoattractant formylpeptide analogue triggers a specific signalling pathway in human neutrophil chemotaxis Susanna Spisani 1 , So a Falzarano 1 , Serena Traniello 1 , Marianna Nalli 2 and ... phosphatidylinositol-3-kinase, as well as activating tyrosine phosphorylation. An increase in intracellular levels of cAMP [6,7] and the involvement of kinases,...

Ngày tải lên: 07/03/2014, 16:20

... Katayama H, Sasai K, Kawai H, Yuan ZM, Bondaruk J, Suzuki F, Fujii S, Arlinghaus RB, Czerniak BA & Sen S (2004) Phosphorylation by aurora kinase A induces Mdm2-mediated destabilization and ... anticancer drug cisplatin. Nature 377, 649–652. 29 Zlatanova J, Yaneva J & Leuba SH (1998) Proteins that specifically recognize cisplatin-damaged DNA: a clue to anticancer activity of cisplat...

Ngày tải lên: 19/02/2014, 05:20

... oxidase is activated by PI3-kinase in the early phase and by PLD in a later phase. Brefeldin A, an inhibitor of ARF GTPases and therefore ARF activation, likewise caused significant inhibition ... (sense: 5¢-ATGAGACACCCGGATCATGT; antisense: 5¢-ACT CACTGGACGGGTGAAAG; 496 bp product) and PLD2 (sense: 5¢-CTGCACCCCAACATAAAGGT; anti- sense: 3¢-GTTCTCCAGAGTCCCTGCTG; 594 bp prod- uct). For a...

Ngày tải lên: 07/03/2014, 15:20

... start from the same train- ing data as above, and create N copies, where N is the number of unique dialogue acts labels in the training set. Each utterance-label pair in the original training ... present in all N training sets. If in the original training set an ut- terance was labeled with the i th dialogue act la- bel, now it will be labeled as a positive example in the i th tra...

Ngày tải lên: 07/03/2014, 22:20

... several important pathways involved in NOS-mediated neurotoxicity, including activation of nuclear factor kappa B, the Jun N-termi- nal kinase pathway and protein kinase C [26,44,45]. Protein kinase ... curcumin, apigenin, naringenin and gallotannin were obtained from Sigma-Aldrich (Castle-Hill, Australia). Phenazine methosulfate was obtained from ICN Biochem- icals (Aurora, OH, USA). Bra...

Ngày tải lên: 22/03/2014, 21:20

... protein acts both as a translational repressor and as the principal viral structural protein, interacting specifically with a 19-nucleotide RNA hairpin to repress translation of the viral replicase ... protein–protein and protein–nucleic acid interactions in MS2 bacteriophage stability Sheila M. B. Lima 1 , Ana Carolina Q. Vaz 1 , Theo L. F. Souza 1 , David S. Peabody 2 , Jerson L. Silva...

Ngày tải lên: 30/03/2014, 11:20

Ngày tải lên: 16/02/2014, 15:20

... the following validated siRNAs: Hs_MAPK14_6_HP validated siRNA against human p3 8a; Hs_MAPK3_7_HP validated siRNA against human ERK1; and Hs_MAPK1_10_HP validated siRNA against human ERK2. HepG2 cells were seeded ... cooled for annealing, and used to knock down HIF- 1a. Similarly, the siRNA against HIF- 2a was prepared using the sense (5¢-CAGCAUCUUUGAUAGCAGUdTdT- 3¢) and antisense (5¢-...

Ngày tải lên: 18/02/2014, 13:20

... examination of the articular cartilage after experimentally induced OA Articular cartilage of the femoral condyles from the experimental joints was examined to assess any macro- scopic damage caused ... proteasome inhibition enhanced TRAIL-induced apoptosis in canine chondrocytes, we examined caspase-8 activation and death receptor-5 (DR-5) and TRAIL protein A B Fig. 1. Evaluation of arti...

Ngày tải lên: 18/02/2014, 14:20

... GTT ACT TCC GCA GCT AGG 466–483 Probe amplification for FISH RpCAbrF TAC AAG GAT GCC ATT AGC 613–630 RpCAbrR1 CGT AGC AGT ATC AGC AGT 822–839 RpCAtrFprobe TAC AAA GAT CCA ATC CAG C 616–634 RpCAtrRprobe ... Histidine residues involved in zinc binding in the catalytic site are indicated by a ‘Z’; important amino acids involved in the hydrogen bond network are indicated by an asterisk; fr...

Ngày tải lên: 18/02/2014, 16:20