Báo cáo khoa học: Solution structure of the active-centre mutant I14A of the histidinecontaining phosphocarrier protein from Staphylococcus carnosus ppt

... mecha- nism of transphosphorylation, which implies the A B Fig. 3. Stereo diagram of the active center of HtA. (A) Superposition of the active-site residues of the 20 conformers of the solu- tion structure ... electrostatically with the other charged groups. In the NMR structures, this side chain of D40 is a short distance (£ 3A ˚ ) from the side chains of...

Ngày tải lên: 18/02/2014, 08:20

... [15]. The solution structure of the MBD of rat (r)MeCP2 has recently been determined [17]. The MBD adopts a wedge- shaped structure, composed of an antiparallel b-sheet on one face of the wedge ... cluster in these two regions. Know- ledge of the structure of a larger portion of MeCP2 including the transcriptional repression domain would resolve these speculat...

Ngày tải lên: 21/02/2014, 00:20

... similarity of the structures. The lowest-energy structure from the RICH NMR ensemble is used for the overlay. (D) The surface of the RICH catalytic domain shows several negatively charged patches of ... al. Solution structure of the RICH catalytic domain FEBS Journal 274 (2007) 1600–1609 ª 2007 The Authors Journal compilation ª 2007 FEBS 1609 Solution struct...

Ngày tải lên: 07/03/2014, 10:20

... towards the interior of the protein. The space that in the wild-type molecule is occupied by the large hydrophobic side chain of Fig. 3. Structure ensemble of HPr (I14A) . The average structure of the 10 ... for the calculations. Therefore, we recalculated the structure of the w ild-type protein on the b asis of the restraints used previously [15]...

Ngày tải lên: 07/03/2014, 16:20

... further our knowledge with respect to the mechanisms of action of these proteins, it is crucial that we define the precise boundaries of the STI1-homologous domain and compare the struc- tures of ... N-terminal part of XPCB–hHR23B ABC Fig. 2. NMR structure of the XPCB domain of hHR23B. (A) Stereoview of the 12 superimposed structures of XPCB–hHR23B. All Pro resi...

Ngày tải lên: 07/03/2014, 17:20

... (blue) with the crystal structure of yeast PDI-b¢ (red, Protein Data Bank entry code 2B5E). (C) Superimposition of the solution structure of human PDI-b¢ (blue) with the crystal structure of the human ... catalytic residues of the a domain face away from the a¢ domain. The crystal structures also revealed the presence of a hydrophobic pocket (which face...

Ngày tải lên: 16/03/2014, 04:20

... the prior formation of the Zn 3 NcMT and its titration with Cu(I). The enhanced sensitivity and resolution of the 800 MHz 1 H-NMR spectral data p ermitted the 3 D structure determination of the ... Determining the deviation of the generated structure with a reduced number of NOEs from the published MT1 structure provides a measure of accuracy shown in Fig...

Ngày tải lên: 23/03/2014, 13:20

... although the length of loop I is longer in short neurotoxins. TheNMRstructureofNTX-1isclosesttothatofthe a-cobratoxin crystal. The superposition of these two structures shows an RMSD value of 3.00 ... of the X-ray structure can be observed in the NMR structures but the length of the b-strand in loop I in solution is somewhat shorter than that of the crystal one (...

Ngày tải lên: 23/03/2014, 13:20

... to other proteins of this class, such as the DNA-binding domain of the Mu repressor [21]. Third, the backbone atoms of the region comprising the ÔwingÕ b-sheet (residues 2–4, 35–36 and 41–44 of ... in the immediate vicinity of residue 28 (Fig. 3B), indicating that the overall structure of Xis_wt is not affected by the substitution. The DSC study revealed that...

Ngày tải lên: 23/03/2014, 15:21

... fact, in the structures of Crt, the side-chain of Phe 25 is close enough to the amide proton of Lys38 to induce a ring current effect. Description of the toxin structure and potential surface The ... reported in Fig. 3A, and the analysis of the / and w angle distribution allowed the identification of the type and boundaries of the secondary structure eleme...

Ngày tải lên: 20/02/2014, 11:20