Báo cáo khoa học: pH dependence, substrate specificity and inhibition of human kynurenine aminotransferase I ppt

... hKAT -I, and also provides biochemical information about the s ubstrate specificity and cosubstrate inhibition of the enzyme. hKAT -I is inhibited by Tris under physiological pH conditions, w hich explains ... transamination of kynurenine to KYNA, arbitrarily termed KAT- I and II , have b een described in r at and human brains [14–16]. In addition, KYNA is involved in...

Ngày tải lên: 07/03/2014, 16:20

... and Pro15 anchors this loop within the vicinity of the active site, therefore significantly reducing the size and accessibility of the acceptor binding pocket (Fig. S6). In SlUGT5, this tyrosine ... b-isosalicin isomer over b-salicin (Fig. 4C). It is more difficult to determine why cluster 3 would favour b-isosalicin formation, but the exact positioning of the catalytic histidine is l...

Ngày tải lên: 28/03/2014, 23:20

... [8,9]. Depending on the type of stress, crucifers biosynthesize different blends of phytoalexins that appear to have multiple roles, including microbial growth inhibition and inhibition of certain fungal ... char- acteristics, a significant understanding of the enzymes involved in the detoxification of phytoalexins, includ- ing their substrate specificity as well as the molec...

Ngày tải lên: 18/02/2014, 14:20

... the identification of essential histidine residues within ResBC proposed to act as axial ligands to heme iron [20]. However, little is known about the substrate recognition or specificity of System ... of ResB and ResC. Heterologous expression of ResBC in Escherichia coli lacking its own bio- genesis machinery allowed us to investigate the substrate specificity of Sys- tem II...

Ngày tải lên: 06/03/2014, 09:22

... indicat- ing that there was some substrate inhibition. Initial reaction velocities for matriptase-2 were fitted to an equation (Eqn 1) describing substrate inhibition [49] (a modified form of Michaelis–Menten ... III (SerpinC1) with heparin exhibited the strongest inhibitory effects on TTSPs, totally inhibiting matriptase, matriptase-2 and hepsin, and leaving DESC1 with 8% residual...

Ngày tải lên: 23/03/2014, 04:21

... Solid-Phase Organic Combinatorial Chemistry, Department of Chemistry, Carlsberg Laboratory, Valby, Denmark; 5 Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph ... specificities determined using a series of fluorogenic peptide derivatives in which substitutions were made on positions P 3 to P 3 ¢ by natural amino acids. Carboxydipep- tidase act iv...

Ngày tải lên: 23/03/2014, 13:20

... high similarity between them, have prob- ably retained considerable individuality in ligand binding and intramolecular signalling. Discussion So far, the structures of two tandem GAF domains are ... mouse isoform in this region, and the GAF tandem domain from the cyaB1 AC because this domain, despite its sequence identity with that of cyaB2 (45% identity, 59% similarity), signals only...

Ngày tải lên: 19/02/2014, 02:20

... concentration of the peptides typically yields sigmoidal curves, indicative of co-operativity, with inflexion points corresponding to peptide concentra- tions giving 50% of maximum activity (IC 50 ). With LUVs ... Valencia, Spain 3 ITC-CNR Institute of Biophysics, Trento, Italy 4 Institut fu ¨ r Biologie ⁄ Biophysik, Humboldt-Universita ¨ t zu Berlin, Germany Mitochondria provide a cri...

Ngày tải lên: 19/02/2014, 07:20

... Aggregation of lysozyme with additive (Eur. J. Biochem. 271) 3243 concentration, indicating that intermolecular interaction is the rate-limiting step in both inactivation and aggregation of lysozyme ... ArgE E is a new candidate additive for the prevention of thermal inactivation of lysozyme. pH dependence of the inactivation and aggregation in the presence of additives Figu...

Ngày tải lên: 19/02/2014, 16:20

... mono- meric form is also stabilized by binding to vitronectin while retaining its inhibitory activity. Under conditions of oxidative stress, the polymerized inactive configur- ation of PAI-2 can ... proliferation and differ- entiation, and inhibit apoptosis in a manner independent of urokinase inhi- bition. Despite these recent advances in defining the intracellular function of PAI-...

Ngày tải lên: 20/02/2014, 02:21