Báo cáo khoa học: Phaiodotoxin, a novel structural class of insect-toxin isolated from the venom of the Mexican scorpion Anuroctonus phaiodactylus pdf

... Phaiodotoxin, a novel structural class of insect-toxin isolated from the venom of the Mexican scorpion Anuroctonus phaiodactylus Norma A. Valdez-Cruz 1 , Cesar V. F. Batista 1 , Fernando ... tran- scriptase. The cDNA was joined with the a daptor provided by the kit (5 ¢-gcugauggcgaugaaugaacacugcguuugCUGG CUUUGAUGAAA-3¢) using T4 DNA ligase. The mod...

Ngày tải lên: 07/03/2014, 16:20

... procedure The assay was run as a three-step assay: initial incubation of the sample and probe, addition and incubation of the sample and acceptor beads in the plate wells, and addition of donor beads ... between the addition of reagents. The broad analytical range of the assay allows quanti- tation of large differences in the DNA-binding activity of transcripti...

Ngày tải lên: 18/02/2014, 14:20

... 5¢-GGGAATTCCATATGAGAGACAATA TTTCCCGTTTATCAAATC-3¢ and 5¢-CCGCTCGAGT CATTACTAGGTTCCCTGTCCAGTGTTACC-3¢,and PDE1(Lys321–Thr620) was amplified with primers 5¢-GGG AATTCCATATGAAGAATGATCAATCTGGCTGCG GCGCAC-3¢ and 5¢-CCGCTCGAGTCATTACTAGG TTCCCTGTCCAGTGTTACC-3¢. ... explore the PDEs of parasites as potential targets for antiparasitic drugs. The African trypanosome Trypanosoma brucei is the pro...

Ngày tải lên: 19/02/2014, 12:20

... a loss of both epithelial and planar polarity [47,48]. The loss of epithelial polarity is a prevailing characteristic of carcinomas, and muta- tion of Rabenosyn indeed causes epithelial tumours ... landscapes of human breast and colorectal cancers. Science 318, 1108–1113. 87 Tsukazaki T, Chiang TA, Davison AF, Attisano L & Wrana JL (1998) SARA, a FYVE domain protein that...

Ngày tải lên: 06/03/2014, 01:23

... 276 93QEGYGSTQLPSDEPLNGLND CAA GAA GGA TAT GGC AGC ACC CAA CTT CCA TCG GAC GAG CCA TTG AAC GGG CTC AAC GAC 336 113KAIQDILNEHNMFRAKERVP AAG GCC ATA CAG GAC ATC CTC AAC GAG CAC AAC ATG TTC CGC GCC AAG GAG CGC GTC CCG ... GAG CAG GTA GCG TCC GAG TCA GCC TCC CTC ACA ACA CCA 216 73TTREEQPAAEAAASTAGGSQ ACC ACC AGG GAA GAA CAA CCG GCG GCA GAG GCC GCG GCG TCC ACC GCT GGC GGT AGC CAA 276 93QEGYGSTQLPS...

Ngày tải lên: 07/03/2014, 12:20

... M, Teramoto T, Kumagaye KY, Nakajima K, Watanabe T, Kawai T, Kawakami Y, Niidome T, Sawada K, Nishizawa Y et al. (1994) Omega-agatoxin- TK containing D-serine at position 46, but not syn- thetic ... Kubota I, Takao T, Shimonishi Y, Yasuda-Kamatani Y, Minakata H, Nomoto K, Muneoka Y & Kobayashi M (1991) Fulicin, a novel neuropeptide containing a D-amino acid residue isolated from...

Ngày tải lên: 18/02/2014, 17:20

... A pair of degenerate primers (abrin 1: 5¢-ACTGAAGGTGCC ACTTCACAAAGCTAYAARCARTT-3¢; abrin 3: 5¢-GGT TAAACACTTCCCGTTGGACCTDATNGT-3¢) was cho- sen to represent the possible coding sequences of the ... obtained previously. Thus, the sequences of the primers used for 5¢ RACE were: 5¢-GGGCATCACGGA AGAAATAG-3¢ for a reverse transcription and 5¢-GC TCTAGAGCATTCGTCACATCGATACC-3¢ with...

Ngày tải lên: 07/03/2014, 16:20

... Tsubouchi H, Naka D, Takahashi K, Okigaki M, Arakaki N, Nakayama H, Hirono S, Sakiy- ama O, Takahashi K et al. (1989) Molecular cloning and sequence analysis of cDNA for human hepatocyte growth factor. ... Yokohama, Japan 2 Advanced Medical Research Laboratory, Mitsubishi Tanabe Pharma Corporation, Kamoshida-cho, Aoba-ku, Yokohama, Japan Introduction Type II transmembrane serine proteases (...

Ngày tải lên: 15/02/2014, 01:20

... Marburg, Germany Introduction Bacterial growth is strongly influenced by the availabil- ity of iron as an essential trace element employed as a cofactor [1]. The fact that the bioavailability of ... residue pattern, leading to the assumption that both activate the same sub- strate (Table S 2A) . On the basis of the bioinformatic analysis of the etc gene cluster, it wa...

Ngày tải lên: 16/02/2014, 09:20

... in calcium signalling. Acknowledgements This work was supported by a grant from the Cana- dian Institutes of Health Research (GSS) and an award from the Canada Research Chairs program (GSS). A. ... truncated and mutant forms of the annexins (particularly annexin A5 ), as well as annexin–protein complexes. In particular, vertebrate structures of human annexins A1 , A2 ,...

Ngày tải lên: 18/02/2014, 14:20