Báo cáo khoa học: Radical-induced oxidation of metformin pptx
Tài liệu Báo cáo khoa học: Critical roles of Asp40 at the haem proximal side of haem-regulated phosphodiesterase from Escherichia coli in redox potential, auto-oxidation and catalytic control doc
... containin g 1 m M EDTA. The auto -oxidation rates of Asp40Ala and Asp40Asn mutants were meas- ured at 25 °C. Oxidation of t he sample was observed b y Fig. 1. Structure of ferric Ec DOS PAS (PDB code ... region of the Fe(III) species, spectra of the Asp40 mutants were essentially similar to that of the wild-type protein. Additionally, spin states of the Fe(III), Fe(II) and Fe...
Ngày tải lên: 19/02/2014, 16:20
Báo cáo khoa học: Thioredoxin Ch1 of Chlamydomonas reinhardtii displays an unusual resistance toward one-electron oxidation potx
... selective and provides a simpler model of oxidation than OH radicals allowing the determination of the main process of o xidation of OH • radicals, without all of the side- effects. They are known ... for methionine. Any of these transformations may affect the function of the enzyme and thus the redox homeostasy. The aim of this work was to determine the sensitivity of T...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: "Circadian pattern of activation of the medical emergency team in a teaching hospita"
... 08:00–18:00 (47% of calls during 42% of the day), a substantial proportion of MET calls occur after normal working hours (53% of calls during 58% of the day), with the peak time of activity occurring ... time, a detailed analysis of the level of utilization of a MET service over a 24-hour period and found a significant increase in the number of MET calls around periods of...
Ngày tải lên: 25/10/2012, 10:45
Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc
... derivative of glycine. GO catalyzes the deaminative oxidation of glyphosate, yielding glyoxy- late, AMPA, and hydrogen peroxide, using 1 mol of dioxygen per 1 mol of herbicide (Fig. 3B). The efficient oxidation ... however, was devoid of substrate or inhibitor, and consequently did not reveal the nature of the active site or the mode of action of glyphosate. A dec- ade late...
Ngày tải lên: 14/02/2014, 14:20
Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt
... of tau with Fyn-SH3. We also show that tau interacts with the SH2 domain of Fyn, and that this associa- tion, unlike that of Fyn-SH3, is influenced by Fyn-mediated tyrosine phos- phorylation of ... SH3 domain of Fyn binds to proline-rich motifs within the sequence PXXP in interacting pro- teins. Seven such motifs are present in the proline-rich domain of the longest isoform of ta...
Ngày tải lên: 14/02/2014, 14:20
Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt
... diffusion method. Two microlitres of protein solution at a concentration of 9 mgÆmL )1 and 2 lL of crystallization solution were equilibrated against 500 lL of reservoir solution. Initial screens ... reactions were started by addition of substrate, and the rate of substrate oxidation was measured by monitoring the change in absor- bance over 5 min. All of the measurements were...
Ngày tải lên: 14/02/2014, 18:20
Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt
... reservoir consisted of 500 lL of 0.7 m sodium citrate, 10 mm dithiothreitol and 70 mm Hepes at pH 7.4. Drops consisted of 0.75 l L of importin-a (70-529) at 18 mgÆmL )1 , 0.75 lL of CLIC4 NLS peptide ... Luria–Bertani medium containing 30 lgÆmL )1 of kanamycin. Flasks of 400 mL of Luria– Bertani medium (30 lgÆmL )1 kanamycin) were then inocu- lated with 10 mL of the overnigh...
Ngày tải lên: 14/02/2014, 19:20
Tài liệu Báo cáo khoa học: PCR detection of nearly any dengue virus strain using a highly sensitive primer ‘cocktail’ ppt
... comparison. Effect of geographical variation in dengue virus on the performance of the primer cocktail Each of the four serotypes of dengue virus can be classi- fied into several genotypes, defined as a group of ... Yuriy Fofanov 1,3 and Richard C. Willson 1,7 1 Department of Biology and Biochemistry, University of Houston, TX, USA 2 Department of Chemical and Biomolecular Engin...
Ngày tải lên: 14/02/2014, 19:20
Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... (Fig. 3A). Three of the ligands, OD2 of Asp165, NE2 of His79 and NE2 of His169, formed an equatorial plane. The other protein ligand, NE2 of His31, bound to the metal, in the company of a water oxygen, ... conformation around the active site of ApeSOD is independent of the presence of a metal cofactor. Figure 3D shows the superimposition of the active site structures of...
Ngày tải lên: 14/02/2014, 22:20
Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt
... quantitation of microgram quantities of protein uti- lizing the principle of protein-dye binding. Anal Bio- chem 72, 248–254. 48 Laemmli UK (1970) Cleavage of structural proteins during the assembly of ... report data on homologous expression of the nrdF gene of C. ammoniagenes strain ATCC 6872. This is the first report of the successful purification of high amounts of the n...
Ngày tải lên: 15/02/2014, 01:20