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Báo cáo khoa học: Investigation of the contribution of histidine 119 to the conduction of protons through human Nox2 pptx

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Báo cáo khoa học: "Constituent-Based Morphological Parsing: A New Approach to the Problem of Word-Recognition" pdf

... number of clitics which are suffixes as far as the phonology is concerned (i.e., they undergo Vowel Harmony 3 with the word to which they attach) are separate words from the point of view of the ... Harmony 4, and, of course, reduplication. The input consists of the stream of segments and a stream of stressesS: pangupangu r nu 1 2 There is a question of course as to whether one could ... suffixation, involves the attachment of an affix to a word; but, unlike these other two processes, an infixed affix occurs within the word rather than at the edge of the word. [3] Vowel Harmony...
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Báo cáo khoa học: A new rice zinc-finger protein binds to the O2S box of the a-amylase gene promoter pptx

Báo cáo khoa học: A new rice zinc-finger protein binds to the O2S box of the a-amylase gene promoter pptx

... pLGD17-265UP1wasusedasthebaitwithaninsertion of three copies of the O2S domain at the 5¢ end of the CYC1 mini-promoter region. Following transfor-mation of the URA-marked plasmid pLGD-265 UP1containing the ... abolished the binding o f the protein to the O2Ssequence completely.RAMY mRNA accumulation in GA-treated tissues To determine whether the expression of the RAMY gene isinduced by GA, and the possible ... the DNA sequenceflanking the GAL4 activating domain and the ADC1terminator region in the pPC86 vector, respectively, wereused to amplify the rice cDNA inserted into vector pPC86.PCR was carried...
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Báo cáo khoa học: Light-harvesting complex II protein CP29 binds to photosystem I of Chlamydomonas reinhardtii under State 2 conditions doc

Báo cáo khoa học: Light-harvesting complex II protein CP29 binds to photosystem I of Chlamydomonas reinhardtii under State 2 conditions doc

... the X-ray structure of the LHCIIprotein [30]. In order to further test the hypothesis thatphospho-CP29 plays a role in the binding of phospho-LHCII to facilitate the State 1 to State 2 transition, ... 5).Discussion The recent X-ray structure of the higher plant LHCI–PSI supercomplex revealed several unique features of the organization of the LHCI antennae and its bind-ing to the PSI core. First, the ... 2005)doi:10.1111/j.1742-4658.2005.04894.x The State 1 to State 2 transition in the photosynthetic membranes of plantsand green algae involves the functional coupling of phosphorylated light-harvesting complexes of photosystem...
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Báo cáo khoa học: Investigation of the contribution of histidine 119 to the conduction of protons through human Nox2 pptx

Báo cáo khoa học: Investigation of the contribution of histidine 119 to the conduction of protons through human Nox2 pptx

... conduction of protons through Nox2. Mutation of individual histidine residues in human Nox2 To investigate the individual roles of His111 and His119, in the conduction of protons through Nox2, two ... contribution of His119 and/or His111 to the conduction of protons through Nox2 has not previouslybeen investigated. To determine whether the mechanism for the conduction of protons through Nox2 requires ... forwild-type Nox2 [13,14]. To assess the contribution of histidines 119 and 111 to the conduction of p rotons th rough Nox2, the ability of CHO91H111/115L, CHO91H115/119Land CHO91H111/119L cells to conduct...
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Tài liệu Báo cáo khoa học: Investigation and prediction of the severity of p53 mutants using parameters from structural calculations pptx

Tài liệu Báo cáo khoa học: Investigation and prediction of the severity of p53 mutants using parameters from structural calculations pptx

... of a severe mutant. At the otherend of the spectrum, where we have low energy, thereis 75% probability for the mutation to be nonsevere if the energy is 0.125 or lower. Thus, on the basis of ... that they comple-ment each other.Other classification techniquesOther classification techniques were investigated to evaluate whether they could add improvements to the new method. To further ... issimilar to that of PREDMUT. However, the weightsfor the individual parameters are not known, makingit impossible to determine the contributions of eachparameter to the final classification.Furthermore,...
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Báo cáo khoa học: Investigation of the kinetics and order of tyrosine phosphorylation in the T-cell receptor f chain by the protein tyrosine kinase Lck potx

Báo cáo khoa học: Investigation of the kinetics and order of tyrosine phosphorylation in the T-cell receptor f chain by the protein tyrosine kinase Lck potx

... recombinant Lck on to each of the peptides. The rates of phosphorylation were found to depend on the location of the tyrosine, leading to the pro-posal that Lck phosphorylates the six f chain ITAM ... radioactivity of the phosphate at the time of the assayfrom the Ôtotal radioactivityÕ samples, to allow conversionfrom c.p.m. to mol phosphate. The phosphorylation wasshown to occur at a constant ... structure. The two groups of peaks at 6.85 and7.15 p.p.m. correspond to the aromatic resonances of the seven tyrosines in the protein, and the inset 2D expansion of the 1H-1H COSY shows that the...
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Báo cáo khoa học: Investigation of the substrate specificity of a b-glycosidase from Spodoptera frugiperda using site-directed mutagenesis and bioenergetics analysis pdf

Báo cáo khoa học: Investigation of the substrate specificity of a b-glycosidase from Spodoptera frugiperda using site-directed mutagenesis and bioenergetics analysis pdf

... affected (Table 6).Thus, the active site of the E451Q mutant is better designed to stabilize the ESàcomplex of glucosides (which p resentsequatorial 4-OH) than that of the galactosides (whichpresents ... structure, in order to increase the catalytic activity of the mutant b-glycosidases. However, the identification of these residues is not an easy task owing to the large number of amino acid residues ... concentrations, and the data were fitted to a Michaelis–Menten equation by using the ENZFITTERsoftware (Elsevier-Biosoft, Cambridge, UK).Calculation of the energy of noncovalent interactionsin the ESàcomplexDifferent...
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Báo cáo khoa học: Investigation of the interaction between the atypical agonist c[YpwFG] and MOR docx

Báo cáo khoa học: Investigation of the interaction between the atypical agonist c[YpwFG] and MOR docx

... effect, togetherwith the steric hindrance, can be used to direct the binding to the receptor and to score the relative values of the binding energy.Conclusions The recent discovery of the atypical ... about 5 A˚towards the bottom of the receptor cavity, leading to greater steric hindrance. Forall the studied peptides, the shift of the cyclic back-bone can also be related to the small difference ... order to assess the contribution of the QM ⁄ MM polarization to the ligand, the interactionenergy between compound 3 and the environment wascalculated. Despite the small movements of the bindingsite...
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Báo cáo khoa học: Investigation of the effects of sulfonylurea exposure on pancreatic beta cell metabolism pptx

Báo cáo khoa học: Investigation of the effects of sulfonylurea exposure on pancreatic beta cell metabolism pptx

... reduced the amount of insulinreleased (Fig. 1).Effects of tolbutamide and glibencamide on[U-13C]glucose metabolism To probe further the effect of tolbutamide and to investigate the observed ... use of a PC inhibitor, the importance of flux through PC for responsiveness to glucose [32]. In the present study, the observeddecrease in flux through PC observed after prolongedexposure to tolbutamide ... level. For seeding of the bioreactor, the cells were pumped into the mini-bio-reactor as a slow rate over a period of 2 h. Typically1 · 108 to 3 · 108cells were passed through the carriers,and...
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Tài liệu Báo cáo khoa học: A novel c-N-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine by Arthrobacter nicotinovorans pAO1 ppt

Tài liệu Báo cáo khoa học: A novel c-N-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine by Arthrobacter nicotinovorans pAO1 ppt

... but the covalent tethering of the flavin cofactor is anautocatalytic process [39] for which the active site has to be well defined [40]. The results of this work define a demethylating oxidase of novel ... mutagenesis of the MABOgene The amino acid substitutions in the MABO protein weremade with the aid of the Quick Change site-directedmutagenesis kit (Stratagene), according to the instructions of the ... probably transferred to tetrahydrofolate, the assumed second cofactor of the enzyme. Methylene-tetra-hydrofolate may then be turned over by the bifunctionalenzyme methylene-tetrahydrofolate dehydrogenase/cyclo-hydrolase...
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